FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15411088
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC7320434.750105897779573No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA4250632.758163472948828No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG3196392.074084581179473No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC2519821.635069503204446No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC2044481.326629242529794No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC1942911.2607221501817394Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA1897301.2311265758783547No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1652161.0720592861451443No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC1622351.0527160704033356No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1441560.9354044308876829Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA1235010.801377553615942Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGAAGATCGGAAGAGCACACGTCTGAACTC1178980.7650206137295433Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC1129230.7327386619296444Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1030050.6683824010348912Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1013110.6573903153365941No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG960310.623129269004239No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC932230.6049086216365775Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC888810.5767341020958416Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT832220.540013787475615No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG816910.5300793818061386No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC815680.5292812551586235No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCAC735930.47753279976079555No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC707750.45924726404780764No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA706950.4587281572851962No Hit
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT695270.4511491985510692Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC694460.4506236029539252No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA679030.440611331270057No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC675210.4381325964785874No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC637500.41366320145599067No Hit
TGAAATGTTTAGGACCACTAGTAGATCGGAAGAGCACACGTCTGAACTCC629840.40869275420398615Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA624630.4053120714124791No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC613200.3978953335416682Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA553930.35943601126669317No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC550930.35748936090690026Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCCA549430.3565160357270038Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC507980.32961981658919864Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG506720.32880222343808563No Hit
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG492000.3192506590060351Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC489590.3176868498836682No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC485170.31481878502023997No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC482580.3131381768762854No Hit
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC481380.3123595167323683Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCGAGATCGGAAGAGCACAC465110.3018021829477581No Hit
GTGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCC447990.2906932982278733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA447940.29066085405521014No Hit
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC445340.28897375707672296Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA441020.2861705805586212No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC438060.28424988553695885No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA426190.27654763894671164No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC397990.2582491255646584No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC396430.25723686737756607No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA382420.24814601019733326No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA370680.24052811845601038No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG366920.23808831667173658No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG363100.23560958188026696Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT358060.23233920927581495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA341170.22137956775018092Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCACA340070.2206657959515902No Hit
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC338840.2198676693040751Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC332420.21570183753411828Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG327600.21257421928938436No Hit
AACCCGTAGATCCGATCTTGTGAGATCGGAAGAGCACACGTCTGAACTCC320720.20810990113092598Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG317010.20570254351931547No Hit
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA309080.20055689773492955Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC296640.19248478757632168Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC296140.19216034584968952Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTAGATCGGAAGAGCACACGTCTGAACTC285630.18534058075588172Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGATCGGAAGAGCA284060.18432183373425678No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC282150.183082466338522No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG280920.18228433969100688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCCAGATCGGAAGAGCACA276540.17944223016570926No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA275850.17899450058295688No Hit
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT273100.17721007108648007Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACAC268540.17425116253959488No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC260950.16932613712931885No Hit
GTGAAATGTTTAGGACCACTAGATCGGAAGAGCACACGTCTGAACTCCAG244030.1583470291000869Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG242520.1573672150856578No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA235450.1527796090710792Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC235090.15254601102790408Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACAC234840.15238379016458797No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGGAAGAGCACACG232550.15089784705661274No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAAGATCGGAAGAGCACA232260.1507096708551661No Hit
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC231610.1502878966105443No Hit
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA231490.15021003059615257Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC231070.14993749954578156Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT228420.14821795839463117No Hit
AGCTACATCTGGCTACTGGGTCTCTAGATCGGAAGAGCACACGTCTGAAC226360.14688125848090675Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG224500.14567433525783513No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGGAAGAGCACACGTC221040.14342919850954067No Hit
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC220180.14287115873973338Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC218740.14193676656703277Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC215420.1397824735021953Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAGATCGGAAGAGCACAC212770.13806293235104491No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGATCGGAAGAGCACA210360.13649912322867797No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACACG209350.13584375094088103No Hit
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC206890.13424749764585084Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT198760.12897207517081208No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCGAGATCGGAAGAGCACACG195510.1268632039477031No Hit
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC195110.12660365056639739Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC192890.12516312930015067Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA189530.12298288089718261No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC186960.12131525042229335No Hit
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC185770.12054307911290885Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTC184600.11978388547258961No Hit
TGAAATGTTTAGGACCACTAGATCGGAAGAGCACACGTCTGAACTCCAGT184250.11955677626394709Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA182210.11823305401928792Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGT180120.11687688760196555No Hit
CAAGTCACTAGTGGTTCCGTTTAGAGATCGGAAGAGCACACGTCTGAACT177470.11515734645081514Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT177400.11511192460908666Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC175260.11372331401910106Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC174380.11315229658022848Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCAC174000.11290572086798804No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG170170.11042049724198577No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT168280.10919410751531625No Hit
ACCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC166430.1079936731267773No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCCAGATCGGAAGAGCAC163900.10635199799001861No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTG162120.10519698544320816Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT160410.10408739473812621Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG160070.10386677436401634TruSeq Adapter, Index 4 (100% over 49bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT158420.10279611666613026Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCAGTGATGACCCTCATCTATCACCCTTGACTGATGAGATCGGAAGAGC157660.10230296524164938No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCTAGATCGGAAGAGCACAC156720.10169301479558096No Hit
TAATACTGCCGGGTAATGATGGAGATCGGAAGAGCACACGTCTGAACTCC156320.10143346141427523Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACC207.911411E-570.036271
TCCCTGG2278050.069.919441
CCTTCAT233000.069.8544542
GTCTACG28600.069.791381
CTTCATT232250.069.763323
CCCTGGT2367450.069.7341162
CCACCGG234500.069.7084110
GTTTCCG301550.069.69951
TCCACCG232950.069.6764769
TTCCACC232300.069.660538
CACCGGA234200.069.6333311
AGCTTAT211450.069.623952
ATTGGTG698150.069.623413
ATTCCAC233000.069.616467
GCTTATC211800.069.607833
CCTGGTG2183750.069.5973363
TCCTTCA229950.069.579411
GCATTGG695800.069.558151
ATAGGCT254900.069.5566916
GGTGGTC2222250.069.550156
Produced by FastQC (version 0.11.5)