FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24205429
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3398621.4040734415407385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG26350.021.8561489
ACTATCG27000.014.88905670
CGACCGT17150.014.5598971
CGACCTA14250.014.11831954
GCGATAG47700.013.59056166
TAGGTCG11200.013.3158843
TAACGGG14850.013.10301369
CTAACGG14100.013.03986768
ATAGGCG52200.012.70300262
GACCTAG52500.012.6205685
GCGTTCG12200.012.51442415
TGTTACG43250.012.40245558
ACTAACG14250.012.36630867
CCTAACT70900.012.3307557
ATCGCGG48450.012.24493810
CGATAGA52700.012.238420567
CGCTCGA15450.012.12786118
TACGCAC17500.012.0931089
GATATCG47350.012.089257
TTACGGT44650.012.02506160
Produced by FastQC (version 0.11.5)