FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17514304
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG857572648.964126693244566No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG7987084.560318240450776No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC6775143.8683466953639725No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT6759383.859348336080041No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG6317303.6069375066231575No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2204511.2586911817906097No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA1608650.9184778338893741No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAGATCGGAAGAGC1065200.6081885983022791No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC831620.4748233215547703Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGAGATCGGAAGAGCACACG670250.3826872024146663No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGCGCACACG538720.3075885858781485No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTCAGATCGGAAGAGCACAC519790.29678027742352764No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC496260.28334554430481507Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC494940.28259187461859747Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACGT489630.279560067017222No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAAGATCGGAAGAGCACA466230.2661995589433642No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAAGATCGGAAGAGCACAC438190.2501897877300748No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTAGATCGGAAGAGC401810.2294181944084104No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTATATCGGAAGAGCACACG382210.21822734149184575No Hit
GGCCGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG360700.20594595137779953No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGCTCGGAAGAGCACACGT353620.2019035412426323No Hit
GGCTGGTCCGATGGTAGTGGGTTATCGGAACTAGATCGGAAGAGCACACG342420.19550876814745252No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAAAGATCGGAAGAG295210.16855365762750266No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC267360.15265236917207786No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC264510.15102512780410798Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGCCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG263910.1506825506740091No Hit
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC256760.14660017320699698Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTAGATCGGAAGAGCACACGTCTGA248800.14205531661435133Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG248770.1420381877578464No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGGACTAGATCGGAAGAGCACACG240280.1371907213669467No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTAGATCGGAAGAGCACA232540.13277147638867065No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG231760.13232612611954206No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATAGATCGGAAGAGCACACGT230220.13144684481895486No Hit
GGCTGGTCCGGTGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG230110.13138403901177004No Hit
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG227560.12992808620884963No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT220710.12601699730688698No Hit
TGAGGGGCAGAGAGCGAGACTTTAGATCGGAAGAGCACACGTCTGAACTC217100.12395582490745849Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAACAGATCGGAAGA215720.12316789750823097No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC212870.12154065614026113Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTACATCGGAAGAGCACACG212430.12128943291152192No Hit
GGCTGGTCCGATGGTAGTGGGTTACCAGAACTAGATCGGAAGAGCACACG208830.1192339701309284No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTATATCGGAAGCGCACACG207750.11861733129675037No Hit
GGCTGGTCCGATGGTGGTGGGTTATCAGAACTAGATCGGAAGAGCACACG202390.11555697560120004No Hit
GGCTGGTCCGATGGTAGTGGGTTGTCAGAACTAGATCGGAAGAGCACACG185250.10577068891804092No Hit
GGCTGGTCCGATGGTAGTGGGCTATCAGAACTAGATCGGAAGAGCACACG179930.10273317169783053No Hit
GGCTGGTCCGATGGCAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG178750.10205943667530265No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCAC150.002225620469.9985369
GGCTGGT14284000.069.981281
ACTATAT130750.069.5702430
GCTGGTC14408300.069.547252
CTGGTCC14412450.069.541353
TGGTCCG14488700.069.52234
GGTCCGA14552400.069.493135
GTCCGAT14428850.069.473046
TCCGATG14429050.069.4557657
CCGATGG14481050.069.4511958
CGATGGT14459850.069.4449549
GATGGTA14440100.069.43603510
GGTAGTG14566850.069.42396513
ATGGTAG14445350.069.4215111
AGTGGGT14465650.069.40938616
TAGTGGG14468300.069.4087615
TGGTAGT14456600.069.4074812
GGGTTAT14446100.069.403519
GTAGTGG14476400.069.39773614
GTGGGTT14491100.069.39085417
Produced by FastQC (version 0.11.5)