FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19140036
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC9000194.702284781491529No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG4152952.169771258528458No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG3703621.9350120344601232No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2731741.4272386948488498No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC2669211.3945689548337317Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC2510751.3117791418992106No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC2385721.246455335820685Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC2233051.166690595566278Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1946591.017025255333898No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA1838220.9604057171052344No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1615550.8440684228598108No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG1508670.7882273575660986Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC1376480.719162701679349No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1268750.6628775410871746No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA1194000.6238232780753391No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC1162530.607381302731092No Hit
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1157610.6048107746505805Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1118820.5845443550889873Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTAGATCGGAAGAGCACAC1060170.5539017794950857No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG1014060.5298109157161459Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC994640.519664644308924No Hit
AAACCGTTACCATTACTGAGTAGATCGGAAGAGCACACGTCTGAACTCCA961240.5022143114046389Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC863010.4508925688541025No Hit
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC832090.4347379492912135Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG827580.43238163188407797No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC818980.42788843239375307No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAGATCGGAAGAGCACACGT810980.4237087119376369No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA796450.41611729465921593No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC773430.4040901490467416Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG754310.394100617156624No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA715600.3738759947995918Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG662090.3459188895987448No Hit
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT655320.3423818011627564Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC653340.3413473203498677Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT607180.3172303333180773Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCC589600.3080453976157621Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC587350.3068698512374794No Hit
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC566820.2961436436169712Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC554940.28993675873963876No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC541970.2831603869501604No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA535510.27978526268184656No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT532180.2780454540419882No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA522150.2728051295201326Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT520880.2721415988977241Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCAGATCGGAAGAGCACACG464500.2426850189832454No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG453260.23681251174240214Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC452010.236159430421134Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT450560.23540185608846292No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG448780.2344718682869771Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG445400.232705936394268No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC434500.22701106727280973No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA420350.21961818671605424Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG403750.21094526676961317No Hit
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC402630.21036010590575693Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG374040.19542282992571175No Hit
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT367070.1917812484783205Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC342120.1787457453058082No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC339430.17734031430243913No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATAGATCGGAAGAGCACACGT337570.17636852929639213No Hit
GAGAAGACGGTCGAACTTGACTATCTAGATCGGAAGAGCACACGTCTGAA331840.17337480451969892Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA329380.1720895404794432Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTAGATCGGAAGAGCA327160.17092966805287094No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC323150.16883458317424271No Hit
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA308570.161217042642971Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGT307430.16062143247797445No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA299990.1567342924537864No Hit
CATTGCACTTGTCTCGGTCTGAAGATCGGAAGAGCACACGTCTGAACTCC296690.15501015776563848Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC285080.1489443384536999No Hit
AGAAGACGGTCGAACTTGACTATCTAGATCGGAAGAGCACACGTCTGAAC282960.1478367125328291Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA279100.14581999741275303Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACG278880.14570505510020984No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC276720.14457653057705847Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC272280.14225678572391398No Hit
TGAGAACTGAATTCCATAGGCTGTAGATCGGAAGAGCACACGTCTGAACT264440.13816065967692015Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC263380.13760684671648477Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC262740.13727246907999546Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC260590.13614916920741424No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA257310.13443548382040663No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCTAGATC253600.13249713845888272No Hit
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT248800.12998930618521304Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC243990.12747624926097317Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT235820.12320770974516453No Hit
AATAGGGACTAAGCAATGTGAGATCGGAAGAGCACACGTCTGAACTCCAG234300.12241356285850247Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACAC230150.1202453328718922No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGAGATCGGAAGAGCAC228290.11927354786584518No Hit
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC227460.11883990186852313Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACACG224930.1175180652742764No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA222150.11606561241577602No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAAGATCGGAAGAGCACACG222130.11605516311463573No Hit
CTGACCTATGAATTGACAGCCATAGATCGGAAGAGCACACGTCTGAACTC219220.11453478979872349Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG218760.11429445587249679No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT213950.11178139894825695No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG212490.11101859996501574No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGATCGGAAGAGCACA212290.11091410695361283No Hit
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC211200.110344620041467Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA208820.10910115320577245No Hit
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCC202920.10601860936938676Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGAGATCGGAAGAGCACACGTCTGAACTC200730.10487441089452497Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA199810.1043937430420716Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA198960.10394964774360926No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG198590.10375633567251388TruSeq Adapter, Index 20 (97% over 49bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT197000.10292561623186079No Hit
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG194650.10169782334787666Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC194020.10136867036195751No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG193410.10104996667717867No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC191750.10018267468253457Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTGG1724200.069.910251
GTTTCCG457900.069.7990951
CCCTGGT1797000.069.640272
GCATTGG1278000.069.592271
ATTGGTG1282400.069.581083
TAGTGTA673700.069.5218
CTGGTCC1013700.069.496573
TTTGTGC330650.069.4865912
CCGTTAC143750.069.443744
TCCCTGT698450.069.419491
GTTCGCC425350.069.4179526
TGGTCCG1040900.069.411954
CGTTACC141400.069.409875
TATCACG496050.069.3661820
TAGTTTG423700.069.3659749
TTTCCGT460350.069.358222
GGCTGGT1018700.069.3486561
GATAGGC352350.069.3401715
ATAGGCT365000.069.3151316
AAGTAAT388150.069.291394
Produced by FastQC (version 0.11.5)