FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004965357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14719868
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC8748555.943361720363254No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG4492093.0517189420448605No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA3227192.192404171015664No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA3179972.160325079002067No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC2143101.4559233819216315Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC2106891.4313239765465289No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC2101241.4274856269091543No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC1898881.2900115680385178No Hit
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1733811.1778706167745525Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC1568051.0652609113070852Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC1428730.9706133234346939No Hit
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA1411610.9589827843564902Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA1368880.9299539914352493No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC1338620.9093967418729569No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1177020.7996131487048662No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCACA1163930.7907204059166836No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1117710.759320667821206No Hit
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1098070.7459781568693414Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGAAGATCGGAAGAGCACACGTCTGAACTC1046630.7110321913212808Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTC870780.5915678048199889Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC850100.5775187657932802No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACAC834060.5666219289466454No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC734540.4990126270154053Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA734270.4988292014575131No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTTGGCGCTCAGATCGGAAGAGCACA726320.493428337808464No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG719690.48892422133133256No Hit
TGAAATGTTTAGGACCACTAGTAGATCGGAAGAGCACACGTCTGAACTCC704430.47855728054083096Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG667790.45366575298093703No Hit
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACT609550.4141001807896647Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCAGATCGGAAGAGCACACGTCTGAACTC583540.3964301853793798Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGCGCTAGATCGGAAGAGCACAC567870.3857847094824492No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC564110.3832303387503203Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC560610.3808526000369025Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCCA541440.36782938542655413Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC522730.3551186736185406No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACAC509980.3464569111625186No Hit
TCCCTGTGGTCTAGTGGTTAGGATTTGGCGCTCAGATCGGAAGAGCACAC500450.33998266832284096No Hit
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG473260.3215110352891752No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG462730.31435743853137815No Hit
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC455100.3091739681361273Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCCAGATCGGAAGAGCACACG442230.30043068320993094No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGATCGGAAGAGCACA415770.2824549785364923No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG406130.2759060067658215No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG399660.2715105869155892Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTGGCGCTCTAGATCGGAAGAGCAC394270.26784886929692575No Hit
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC392250.26647657438232464Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACA379840.2580457922584632No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG369340.2509125761182097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCTGTGTGGAATTTGAATATCTGAAAAGATCGGAAGAGCACACGTCTG361860.24583100881067685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGAAATGTTTAGGACCACTAGAGATCGGAAGAGCACACGTCTGAACTCC359720.2443771914259014Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAGATCGGAAGAGCACA354730.2409872153744857No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACAC347160.23584450621432204No Hit
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA345030.23439748236872776Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTCCAGATCGGAAGAGCACA344030.23371812845060838No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA343390.23328334194301198No Hit
TGAGGTAGTAGGTTGTGTGGTTAGATCGGAAGAGCACACGTCTGAACTCC336350.2285006903594516Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA332630.22597349378404752No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC326350.22170715117825787No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT324150.22021257255839524No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCA317200.21549106282746558No Hit
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC315150.21409838729532082Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAAGATCGGAAGAGCACACGTCTGAACTC313740.21314049827077255Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTTGGCGCTAGATCGGAAGAGCACACG313330.21286196316434358No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC299020.20314040859605534No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA298810.20299774427325026Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAATGTTTAGGACCACTAGATCGGAAGAGCACACGTCTGAACTCCAGT290840.19758329354583887Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC289340.1965642626686598No Hit
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTC285750.19412538210261127Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG272410.1850628008348988No Hit
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG265610.18044319419168706Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAGATCGGAAGAGCACACGTCTGAACTCC264730.179845362743742Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGAAATGTTTAGGACCACTAGATCGGAAGAGCACACGTCTGAACTCCAG262660.17843910013323488Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA254960.1732080749637157Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAAGATCGGAAGAGCACACG253540.17224339239998618No Hit
AGTAATGATGAATGCCAACCGCTCTGATGAGATCGGAAGAGCACACGTCT249990.16983168599066242Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCGTAGATCCGATCTTGTGAGATCGGAAGAGCACACGTCTGAACTCC249490.16949200903160275Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGTGATGCGAACTGGAGTCTGAGCAGATCGGAAGAGCACACGTCTGA248430.1687718938783962Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTTGGCGCTCTAGATCGGAAGAGCACA247380.16805857226437085No Hit
TTCACAGTGGCTAAGTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT246210.16726372818017118Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT244740.16626507792053571No Hit
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG239890.16297021141765672Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCC231260.15710738710428654Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACGT230020.1562649882458185No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTAGATCGGAAGAGCACAC228750.1554022087698069No Hit
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC220210.14960052630906745Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG218810.14864943082370033No Hit
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC215660.1465094659816243Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTTCATTCCACCGGAGTCTGAGATCGGAAGAGCACACGTCTGAACTCC213710.1451847258412915Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCCAGATCGGAAGAGCAC213690.14517113876292911No Hit
GCATTGGTGGTTCAGTGGTAGAATTAGATCGGAAGAGCACACGTCTGAAC211290.14354068935944264Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAGATCGGAAGAGCAC211030.1433640573407316No Hit
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACAC210800.14320780593956411No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC208130.1413939309781854No Hit
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC205350.13950532708581354Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGAAGATCGGAAGAGCACACGTCTGAACTCC205310.13947815292908877Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA202960.13788167122150824Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC196260.13332999997010844No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCAGATCGGAAGAGCACA192080.13049030059236946No Hit
TCCCTGAGACCCTTTAACCTGTAGATCGGAAGAGCACACGTCTGAACTCC184840.12557177822518517Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG182700.12411796084040971No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTAGATCGGAAGAGCA182500.12398209005678583No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTCAGATCGGAAGAGCACA178630.12135299039366385No Hit
TGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCAGATCGGAAGAGCAC178090.12098613927787939No Hit
AGCTACATCTGGCTACTGGGTCTCTAGATCGGAAGAGCACACGTCTGAAC177090.12030678535976001Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA171660.11661789358437182Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAAGTCACTAGTGGTTCCGTTTAGAGATCGGAAGAGCACACGTCTGAACT168500.11447113520311458Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGACCTCAGATCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGT168010.1141382517832361Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACACG166330.11299693720079554No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT165320.11231078974349498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGCGGGTGCTCTTATTTTAGATCGGAAGAGCACACGTCTGAACTCCAG164770.11193714508852931Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTCAGATCGGAAGAGCAC163820.11129175886631591No Hit
ATCACATTGCCAGGGATTACCACTAGATCGGAAGAGCACACGTCTGAACT163330.11095887544643743Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCC160330.1089208136920793Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC159780.10854716903711366Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAAGATCGGAAGAGCACA159770.10854037549793245No Hit
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG157420.10694389379035192Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCTTCATTCCACCGGAGTCTGTAGATCGGAAGAGCACACGTCTGAACTC156760.10649552020439312Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT152640.10369658206174132Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCAGATCGGAAGAGCACAC152590.10366261436583533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAC150.002224956270.003743
TCCCTGG2419050.069.9449841
ATTGGTG1052750.069.820883
GCCCGGC372950.069.795671
CCCTGGT2513400.069.789752
CCTTCAT185550.069.740122
CCTGGTG2517200.069.706173
TCCTTCA183050.069.705191
TCCCTGT1301500.069.667181
GCATTGG1053900.069.664921
GATAGGC313900.069.651215
GGTGGTC2572100.069.643126
TAGTGTA579650.069.6187068
TTCCACC183600.069.611828
ATAGGCT276600.069.604616
TCCACCG184450.069.59469
CCGTAGT346050.069.5890355
TAATCCA311500.069.566117
TTGGTGG1068900.069.548574
GTTGACA76300.069.5390918
Produced by FastQC (version 0.11.5)