FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004965362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997065
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN271922.7272043447518466No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG43400.4352775395786634No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG37090.3719917959210282No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG31840.3193372548429641No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT30060.3014848580584014No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA22590.22656496818161304No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG20370.2042996193828888No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT20060.20119049410018403No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC19600.19657695335810604No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT19570.19627607026623137No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG19300.1935681224393595No Hit
CAACATTTTAATTAAAATGATTTACCTTAACGAAGTCTCTATCAGTCTTCTCTTTCCATTCTTCTCCAAATAC18930.18985723097290547No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG18120.18173338749228987No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC17940.17992808894104195No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC17740.1779222016618776No Hit
GTATTTGGAGAAGAATGGAAAGAGAAGACTGATAGAGACTTCGTTAAGGTAAATCATTTTAATTAAAATGTTG16540.16588687798689153No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA16520.16568628925897508No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT16510.16558599489501685No Hit
CGAAAACCTCGTGGGAGACCTAGAAGTGGCTCTGACCGAAATTCAGCTAT15250.15294890503628147No Hit
AGAAGGAGGAGCTGCTGTTGGTATAGTGCTGAGGGTTCCAATGTTACCAG15140.1518456670327411No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG14900.1494386022977439No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC14660.14703153756274667No Hit
ACAAAAGCAAGGTGTCACATCCGGAGATAAACATTTATTACAGGGACTGG14540.14582800519524805No Hit
CCTCCTTAGCCCACTGATGAACGATGGCTCCAACTCTGGTAACATTGGAA14200.14241799682066866No Hit
CCCCCACTGTGTAAGTGGAATTATTTTTCACTGCAGCATTTCTGAGTCTT14020.14061269826942074No Hit
ATATTTTTCTCTTGCAGATTGATTATACCGGAACAGAGCCTTCCAGTCCC13610.13650062934713383No Hit
GAACTTAATTTTACAGTTCAGAGTAAGGAACAAGAAAAAGACTCAGAAAT13540.13579856879942634No Hit
GAAAAAGAGGGACCAATAACTAAACACATCCGACTAACAGCTGCCTTAAT13200.13238856042484692No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA13190.1322882660608887No Hit
GTTTTCTCTGCAGTCTTTAAACCAGAGGAGTTACGCCAGGCCCTCATGCC13160.13198738296901408No Hit
CCATAACAGCTTTTTTCTCTCCTTGAAGTATTTGTCTGAAAGTGGCCACC13140.13178679424109763No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT13030.13068355623755723No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC12950.12988120132589148No Hit
GAACCCAAATTATACCCCTTGCCAAAAATAAAAATCTTACCCCGCTCATT12950.12988120132589148No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA12950.12988120132589148No Hit
GACTGCTTCCTACATCGTAAGTGCAATTATTGTACGTTTTCATTTTCTAT12900.1293797295061004No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA12900.1293797295061004No Hit
GAACCAATCAAGCATTGCCCAAGATCGAGGTGAGAGCCTGGGTGTTGGGG12740.12777501968276894No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC12670.1270729591350614No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG12630.12667178167922855No Hit
GCATTTTAAGACTCTTACCATGTCCTTCATCACACAATCTACATCTTCTG12550.1258694267675628No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC12540.12576913240360457No Hit
TTTCTACAACCAGCTTGCATTTTTTCTGCCCACAATGAGCGGGGTAAGAT12370.12406412821631488No Hit
CTGTAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAG12330.123662950760482No Hit
TATTTAGAGGGGAAAACACAGATGGATCTGAGAGGATAGCTGAATTTCGG12310.12346236203256558No Hit
GAGAGGCTGTGCCGTTACTGCTACTCTCTTCTTCCTCTTTAACTTCTACT12230.12266000712089983No Hit
CCAGTCAATTTTGCTGTGAACCTAAAACTGCTCTAAAAAAGTCTTTTTTT12210.12245941839298341No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG12120.12155676911735945No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT12080.12115559166152659No Hit
CATAAATGAGGATTTTATTGTTTGGATGCGTACTGCAGCATTACCTACTT11980.12015264802194442No Hit
GGCATCTAGATCAGTTACTCGGTTGCGAATCAGAATCTAGAAGAGGAGAAAGTACAGAAAAGAGAGTCACCTGG11940.11975147056611155No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT11800.11834734947069649No Hit
CCAGGTGACTCTCTTTTCTGTACTTTCTCCTCTTCTAGATTCTGATTCGCAACCGAGTAACTGATCTAGATGCC11790.11824705510673829No Hit
CAGAACCAATGGAAGTGGATGAAAAGAAACCTGAAGTGAAAGTAGAAGTT11780.11814676074278006No Hit
CGTGCTAATTGCATTAACCTGCCTGGATGGTACCACTGTGAGTGCAGAGA11780.11814676074278006No Hit
CCAATTTATTTAATTGAAAAGATTGGTTAACATTTTATAGTCAAAGTAAT11690.1172441114671561No Hit
ACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGT11630.11664234528340679No Hit
GACCTTCACACGATTCTCCACTTGGTGAAAACATCCCATTGTCATGGTAG11610.11644175655549037No Hit
CCCCAGGAGGCCAAAGACACAGATCAAGGAGTAGGCAATCGGTACAAATA11600.11634146219153216No Hit
CACGACAGTAATGGCGCCCTTCAGCACACTCATCGATGTCTGTGAAGAAA11560.11594028473569928No Hit
CAGAAAAAAATGAGACTTGGCCTACCTGCCGGTAGCGTAGCAAATGGCTG11540.11573969600778285No Hit
GTAAATCACTTTTCCTTTCTATAAGACGATACAACTTGCGAAAAGTAGGT11510.1154388129159082No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC11470.11503763546007531No Hit
GATACTAGAGCCGCTGCCTCCTCGTAGAAGCTCCGACAGTTGTTTATGTT11460.11493734109611711No Hit
ATCCACACACTGCCCATTGTTCACACAAGGGTTGCTCTGACATTCATTTA11380.11413498618445135No Hit
TTCTCTGAAGCAAGTTCTGACAGCTTTGTTGAATTCTCGGACTACCAGCC11370.11403469182049315No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG11120.11152733272153771No Hit
GCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCG11120.11152733272153771No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC11090.11122644962966308No Hit
TTTCAAAGCGATAGGTATAGGATAAGAATCATTGCCAATGAGAAACAATT11060.1109255665377884No Hit
ATATAGGAGCTCCAGAATATTGCCCACTGTTGACCTCTTTGTCCTGTTTT11060.1109255665377884No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG11040.11072497780987198No Hit
CGCAAAAAGAAAATATATGAAGAGAAAAACTTACTTGCAGTATCCTGTGC10990.11022350599008089No Hit
GGCAATAGCTCCACAAGGAGCAATTGGTTTGTCTTCATTTCTTCGATAAG10980.11012321162612267No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG10970.11002291726216445No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA10870.10901997362258227No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA10840.10871909053070763No Hit
ATCGCCTCTATGTGTCTGTGAAGGAGATTGGTGGATTGACTCAGGTGAGT10820.10851850180279118No Hit
ACACGGATGGCTTTGTGCCTTTTAGAATTCTGAGCTTGCACCAAGGTTTT10780.10811732434695832No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG10740.10771614689112545No Hit
CTTTTCCTTGTAAAAACCAGAATCCCAGTAAGGAATGTGAACCTTATCGA10710.10741526379925082No Hit
GTACCAAGCAATGTGCCTTTAAAAGAGGTCCTATGGAAAAAACAAAAGGA10710.10741526379925082No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC10670.10701408634341793No Hit
CTTTCAGCCAATAGCAATCCTTGTGAGCATGCAGGAAAATGTGTGAACAC10610.10641232015966863No Hit
CCTCACACAACACACCAGTGAAACCTTTGGAAAGAATTTTATCAAGGATT10530.10560996524800288No Hit
CACCCAGCCCAAACTACCAAAAATATACATATGGCCAATCCAGCATGAGC10510.10540937652008646No Hit
CCATTTAGTTGACTATCATCTCGAGATTTCACGTAACGACGATGGTTTTGATAGAAATTAGACAGTCCAT10510.10540937652008646No Hit
TAGTAATGTCTTTCTTTGCTTGTGTTTAGATACATTAGAATTGTTTCTCA10400.10430613851654606No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG10400.10430613851654606No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT10360.10390496106071319No Hit
CTCACAACGAGGTCCTGCATAACCCTTCAGACACTCACAGTGGAAGGCGC10340.10370437233279675No Hit
GACTCATACCTCCATAACTCGACTTATTGTCCTGAAGTGTTTGTATAGGC10320.10350378360488033No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG10310.1034034892409221No Hit
GCAGAGATTCACCAGGGTCTGAAACAGAGGCAGGGGTGTACATACATCAA10310.1034034892409221No Hit
CATCAACACCATCCAACTGTGAGATTTTTGGAAGATCACACTGTTCCTCC10260.10290201742113102No Hit
GGTGTGGAATATCTGCCTTGGTCTTTGAGAATCCTTGATAAAATTCTTTC10230.10260113432925637No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC10210.10240054560133993No Hit
TTTAAAAGATGAGAAAGCTCTGAATTCAGAATTTTCCAGTTCTGCAACTT10180.10209966250946528No Hit
CTGTAGGAAGAGACACCTCTGTTTCTTGCTGCCCGGGAGGGGAGCTATGA10160.10189907378154883No Hit
TATATCTAGTGTTTTAATCAGAGAAAGACTAAAACTTACTTGGTGCAAGG10080.10109671886988311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)