FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004965363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997065
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN266222.6700365572956626No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG42390.4251478088188834No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG31460.3155260690125518No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG29080.2916560103904961No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT28440.2852371710971702No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT25600.2567535717330365No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA23820.23890117494847377No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC20280.20339697010726485No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG20140.20199284901184977No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT20110.20169196591997515No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG18660.1871492831460336No Hit
GTATTTGGAGAAGAATGGAAAGAGAAGACTGATAGAGACTTCGTTAAGGTAAATCATTTTAATTAAAATGTTG18360.1841404522272871No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC18150.18203427058416452No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG17910.1796272058491673No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC16930.16979835818126202No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT16930.16979835818126202No Hit
CAACATTTTAATTAAAATGATTTACCTTAACGAAGTCTCTATCAGTCTTCTCTTTCCATTCTTCTCCAAATAC16470.165184817439184No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA16040.16087215978898065No Hit
CCTCCTTAGCCCACTGATGAACGATGGCTCCAACTCTGGTAACATTGGAA14940.14983977975357676No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC14550.14592829955920628No Hit
ACAAAAGCAAGGTGTCACATCCGGAGATAAACATTTATTACAGGGACTGG14540.14582800519524805No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG14380.14422329537191658No Hit
CGAAAACCTCGTGGGAGACCTAGAAGTGGCTCTGACCGAAATTCAGCTAT14370.14412300100795836No Hit
GAACTTAATTTTACAGTTCAGAGTAAGGAACAAGAAAAAGACTCAGAAAT14320.14362152918816729No Hit
ATATTTTTCTCTTGCAGATTGATTATACCGGAACAGAGCCTTCCAGTCCC13800.13840622226233998No Hit
AGAAGGAGGAGCTGCTGTTGGTATAGTGCTGAGGGTTCCAATGTTACCAG13780.13820563353442353No Hit
CCCCCACTGTGTAAGTGGAATTATTTTTCACTGCAGCATTTCTGAGTCTT13510.13549768570755166No Hit
GACTGCTTCCTACATCGTAAGTGCAATTATTGTACGTTTTCATTTTCTAT13460.13499621388776056No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT13220.13258914915276335No Hit
TATTTAGAGGGGAAAACACAGATGGATCTGAGAGGATAGCTGAATTTCGG13090.13128532242130653No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA13060.13098443932943188No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA12930.12968061259797506No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC12840.12877796332235109No Hit
GTTTTCTCTGCAGTCTTTAAACCAGAGGAGTTACGCCAGGCCCTCATGCC12720.1275744309548525No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA12720.1275744309548525No Hit
AGGGCACTCAAATGTCTGCCCTAGCTCCCTGCCCCTCCCCAACACCCAGG12690.12727354786297784No Hit
TTTCTACAACCAGCTTGCATTTTTTCTGCCCACAATGAGCGGGGTAAGAT12680.12717325349901962No Hit
CCATAACAGCTTTTTTCTCTCCTTGAAGTATTTGTCTGAAAGTGGCCACC12640.12677207604318677No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG12640.12677207604318677No Hit
CTGTAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAG12530.12566883803964637No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC12500.12536795494777173No Hit
GAACCCAAATTATACCCCTTGCCAAAAATAAAAATCTTACCCCGCTCATT12470.12506707185589705No Hit
ACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGT12280.12316147894069092No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG12240.12276030148485806No Hit
CAGAACCAATGGAAGTGGATGAAAAGAAACCTGAAGTGAAAGTAGAAGTT12210.12245941839298341No Hit
GACCTTCACACGATTCTCCACTTGGTGAAAACATCCCATTGTCATGGTAG12030.1206541198417355No Hit
CCAATTTATTTAATTGAAAAGATTGGTTAACATTTTATAGTCAAAGTAAT12000.12035323674986084No Hit
GCATTTTAAGACTCTTACCATGTCCTTCATCACACAATCTACATCTTCTG11960.11995205929402797No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT11900.11935029311027866No Hit
GTAAATCACTTTTCCTTTCTATAAGACGATACAACTTGCGAAAAGTAGGT11900.11935029311027866No Hit
CACGACAGTAATGGCGCCCTTCAGCACACTCATCGATGTCTGTGAAGAAA11840.11874852692652937No Hit
GAGAGGCTGTGCCGTTACTGCTACTCTCTTCTTCCTCTTTAACTTCTACT11780.11814676074278006No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT11730.11764528892298898No Hit
TTTCAAAGCGATAGGTATAGGATAAGAATCATTGCCAATGAGAAACAATT11710.11744470019507253No Hit
CATAAATGAGGATTTTATTGTTTGGATGCGTACTGCAGCATTACCTACTT11690.1172441114671561No Hit
CCAGGTGACTCTCTTTTCTGTACTTTCTCCTCTTCTAGATTCTGATTCGCAACCGAGTAACTGATCTAGATGCC11610.11644175655549037No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC11610.11644175655549037No Hit
GGCATCTAGATCAGTTACTCGGTTGCGAATCAGAATCTAGAAGAGGAGAAAGTACAGAAAAGAGAGTCACCTGG11580.11614087346361571No Hit
TTGATCAAAGGCGAAGTGAGAGAGCTTTAGTTGCTTTAAAAAAAAAAAAA11470.11503763546007531No Hit
GAAAAAGAGGGACCAATAACTAAACACATCCGACTAACAGCTGCCTTAAT11460.11493734109611711No Hit
CAGAAAAAAATGAGACTTGGCCTACCTGCCGGTAGCGTAGCAAATGGCTG11450.11483704673215889No Hit
GCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCG11410.11443586927632603No Hit
AAACAGTGGGAACCTTGTTCCAGATGCTGCTTCCAAACATAAACAACTGT11350.11383410309257672No Hit
TTCTCTGAAGCAAGTTCTGACAGCTTTGTTGAATTCTCGGACTACCAGCC11280.11313204254486921No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA11250.11283115945299453No Hit
ATCCACACACTGCCCATTGTTCACACAAGGGTTGCTCTGACATTCATTTA11180.11212909890528702No Hit
TGCAGGAGGTCAGGCAGTTCTCCAGGCTATTTGTACCGATTGCCTACTCC11110.1114270383575795No Hit
GGAGAGGAACGACTGGCATCCCATAATTTATTTCGGGAGGAGGAACAGTG11110.1114270383575795No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG10980.11012321162612267No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC10980.11012321162612267No Hit
CGTGCTAATTGCATTAACCTGCCTGGATGGTACCACTGTGAGTGCAGAGA10970.11002291726216445No Hit
CTTTTCCTTGTAAAAACCAGAATCCCAGTAAGGAATGTGAACCTTATCGA10940.1097220341702898No Hit
TACTCACCTGCGACCACATTCTGAATATTTACCAATATTTTTTAATATTA10900.10932085671445695No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC10900.10932085671445695No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG10890.10922056235049872No Hit
CCTCACACAACACACCAGTGAAACCTTTGGAAAGAATTTTATCAAGGATT10840.10871909053070763No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC10830.10861879616674941No Hit
ACTACAAGCCAACTGGCTTAACACAGGTCAATAAGAAACTATCACATGGG10820.10851850180279118No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG10800.10831791307487476No Hit
CTTTCAGCCAATAGCAATCCTTGTGAGCATGCAGGAAAATGTGTGAACAC10790.10821761871091654No Hit
CGCAAAAAGAAAATATATGAAGAGAAAAACTTACTTGCAGTATCCTGTGC10760.1079167356190419No Hit
GGCAATAGCTCCACAAGGAGCAATTGGTTTGTCTTCATTTCTTCGATAAG10740.10771614689112545No Hit
ATATAGGAGCTCCAGAATATTGCCCACTGTTGACCTCTTTGTCCTGTTTT10740.10771614689112545No Hit
CACCCAGCCCAAACTACCAAAAATATACATATGGCCAATCCAGCATGAGC10710.10741526379925082No Hit
TTTAAAAGATGAGAAAGCTCTGAATTCAGAATTTTCCAGTTCTGCAACTT10500.10530908215612823No Hit
ATCGCCTCTATGTGTCTGTGAAGGAGATTGGTGGATTGACTCAGGTGAGT10490.10520878779217001No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA10470.10500819906425359No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG10430.10460702160842072No Hit
ACACGGATGGCTTTGTGCCTTTTAGAATTCTGAGCTTGCACCAAGGTTTT10230.10260113432925637No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG10200.1023002512373817No Hit
CTCACAACGAGGTCCTGCATAACCCTTCAGACACTCACAGTGGAAGGCGC10200.1023002512373817No Hit
TATATCTAGTGTTTTAATCAGAGAAAGACTAAAACTTACTTGGTGCAAGG10140.10169848505363242No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC10140.10169848505363242No Hit
TAGTAATGTCTTTCTTTGCTTGTGTTTAGATACATTAGAATTGTTTCTCA10130.1015981906896742No Hit
CTTTCTAGAATTTTAGTCATACCTGTAGACACCAACTGCCTCCTTAGAAG10130.1015981906896742No Hit
GCAGAGATTCACCAGGGTCTGAAACAGAGGCAGGGGTGTACATACATCAA10130.1015981906896742No Hit
ATGGACTGTCTAATTTCTATCAAAACCATCGTCGTTACGTGAAATCTCGAGATGATAGTCAACTAAATGG10120.10149789632571597No Hit
CTGTAGGAAGAGACACCTCTGTTTCTTGCTGCCCGGGAGGGGAGCTATGA10120.10149789632571597No Hit
GGTGTGGAATATCTGCCTTGGTCTTTGAGAATCCTTGATAAAATTCTTTC10100.10129730759779955No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG10090.10119701323384132No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG10070.10099642450592489No Hit
GTACCAAGCAATGTGCCTTTAAAAGAGGTCCTATGGAAAAAACAAAAGGA10040.10069554141405024No Hit
GACTCATACCTCCATAACTCGACTTATTGTCCTGAAGTGTTTGTATAGGC10010.10039465832217558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)