FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147807
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN300051.3970063418174912No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG74120.34509618415434906No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG69390.32307372124217865No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG53170.24755483150953506No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG47610.22166796178613815No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT47610.22166796178613815No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC47450.22092301589481736No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA40360.18791260108566551No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC39580.18428098986547672No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT39450.1836757213287786No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG37880.17636593977019352No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT34770.16188605400764594No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG34640.16128078547094782No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA33760.15718358306868355No Hit
CAGACTCGACTCACCAGAGGCAGGCTGACCAACTCCTGCATTCGACTGTT33530.15611272334990992No Hit
ACAAGTTCAGCAGCCCCAACAAGTACAGATGCAAGTTCAACCTCAACAGT32750.15248111212972115No Hit
TCCTAGGTCAGCGGTGTACCATTGACATTGACGAGTGTATCTCCAAGCCC30890.14382111614311713No Hit
GTAGCTGCCCTGGGTGTTATGGCAGAGACCATGGTTCATGCAGGGCTTGG30660.14275025642434352No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG30540.14219154700585293No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT30200.1406085369867963No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG29510.13739595783047548No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC28990.13497488368368293No Hit
CCTCGGTGTCCGAGGACCTGGGGCTGGTGCATTTTCGGTTGTTGCTGATC28890.13450929250160745No Hit
CCTGCTGCCAAGAGGGTCAAGTTGGACAGTGTCAGAGTCCTGAGACAGAT28760.13390402396490933No Hit
CAAACAGTTGTACAATCTCCTGGGAGGTCCATGACCTCTGCTTGCTCAGT28750.13385746484670177No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA28250.13152950893632437No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC27750.12920155302594694No Hit
ACCCCCATCTCTCCTCCTCCGCTTCTCCCTTGGCATCCCACAGCTCTAAA27310.12715295182481479No Hit
GAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGGTAA27110.12622176946066382No Hit
GAACACACTGGCGGTTGTCAATTTCCAGAAAGACTTTAGAGCTGTGGGAT27050.12594241475141854No Hit
CAGAGTGAAACACCCACTTACTTGCAGCACTGCTTCTTTATATTGCGACC26530.12352134060462602No Hit
ACCACCTCATGTTCCTCCTAAGAGAAGTGGCCTTACCTTGTCTTTCACAT25780.12002940673905989No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT25470.11858607407462587No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG25240.11751521435585227No Hit
GTTCCCAATGGCAAAAGCACTTACCCACAATGGTGGAGAATCTCCGAGTG25160.11714274141019189No Hit
CCCTCTCTTCTCTGCAGAAAAGAAGGACACAGTGTTGCGGCAGGTACGCC24840.11565284962755033No Hit
AGAAAGTGGAGCATGTCATCAAAGATAGGCTGCAAGTCACAGGGGTCCAG24790.11542005403651259No Hit
CTCCGGATGTGACACCTTGCTTTTGTACCATTAACTTCACACTGGAAAAG24590.11448887167236162No Hit
CCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGG24180.11257994782585214No Hit
ACTAATTGCTTAGATAAGCCCAAGTTTGGTGGTCGCAATATAAAGAAGCA24000.11174188369811626No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA23920.11136941075245588No Hit
AGCACATTACTTGGAAACACAGACCTCAAATGACTTTTCCAGTGTGAAGT23790.11076414221575775No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG23770.11067102397934266No Hit
CCCCAGGCACTGATACTCACCTGACTGAGGTTGAAGGTCATGATGCTGTT23760.1106244648611351No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC23590.10983295985160678No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG23540.10960016426056904No Hit
GTTGTCGTCGTTGCAAATTCTGTCACGTTTGTGGAAGGCAACATCAGGCT23420.10904145484207846No Hit
ACAGCATTTGCCATCCACATACGCAAGAGCCGACTTAAGTGGGCAGTCAG23420.10904145484207846No Hit
GAGGACACTACACTTACTTATTGCAGTCAATGTGTAAAATCAAATGGGAA23390.1089017774874558No Hit
CGCCTTGTCTCCTACAGGGAAACCAATGAAAAGCGTGCTGGTGGTGGCTC23380.10885521836924827No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA23290.10843618630538032No Hit
AACCCCCAAGCTGTCTGTAGTCACACACAGGGAAGGGCAGGAGGCAGGGA23130.10769124041405956No Hit
GGCCTTCTTGCTGACAGGTATCATTTCTGTGAGAAGTGTTTCACAGAGAT22930.10676005804990858No Hit
GAAGTGGACTTTGCTATTAACGTATGCACTCTCCTATCAAATGAAAGCAA22880.10652726245887084No Hit
CAGCCCTGCCAGAATGGAGGCACCTGTATTGACCTTGTGAACCATTTCAA22860.10643414422245574No Hit
GCCTGTTCTCTGCTTCCCCTTACCTAGGTATGACAAATACTGTGCAGACC22840.10634102598604064No Hit
TGTTGCCATGACCTTGCTCTACTGAACCACAAGGAGGGCTAGAGATGTGGTCTGCATCCATGTGTCCTTGGATA22810.106201348631418No Hit
CTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGGGTAAGTTCTTGCTTTGTTCAA22770.10601511215858782No Hit
ACCCCGAGTGCCTGGTGGGCAAGAGCACTTGAAATGGTTCACAAGGTCAA22710.10573575744934252No Hit
ACAGAGGCCCAGCTGTAGTTCTATTACCAAGTTTTGTACCTTTGTAGCCT22650.10545640274009724No Hit
GGGGCTGTGAAGGGTCGTCACCCAGGGTCACATTCTCGCCCTGGATCTCT22600.1052236071490595No Hit
TATCCAAGGACACATGGATGCAGACCACATCTCTAGCCCTCCTTGTGGTTCAGTAGAGCAAGGTCATGGCAACA22580.10513048891264437No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA22280.10373371536641794No Hit
ACAGGACCGCCAAGAAAAGAAGTTCCCAAAACCACTCCTAGTGAGCCCAA22220.10345436065717264No Hit
TAGGATCTTTTTCAAGTTGCATGACGTGCTTGCTTTCATTTGATAGGAGA22210.10340780153896509No Hit
CCTTGAAAGCCCTAGACTCTCAAATCCTCATGTGCCCTCAAACAAGCTAC22200.10336124242075755No Hit
CAGAAAACCACCGATCTCGCTGATCGTCAGAAAGGCTAAATTTGGTGTCT22120.10298876947509715No Hit
TGCCCTCCTCACTCACCTGATTCTGGTGGTGGAGGCTGCTTTTTCTTGGG21950.10219726446556883No Hit
CAGGGAAGAGTCGCAGCATGTCAGCCTCTTTTCTCATGGCAGCCACAGGC21850.10173167328349335No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG21680.10094016827396503No Hit
CAGCAAGCCACAAAGATTAAAAAATTAAGAGCAGGTAAACTCTCTCCTCT21650.10080049091934237No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT21650.10080049091934237No Hit
CCACCTCCTGCTCTGCCTGAGGAGGGGTGAGAGCCAGCGATGGTGTCCTA21640.10075393180113484No Hit
GAACAACAAGGCAGATGGGACACCCAAGACAGAATCCAAATCCAAGGTAG21620.10066081356471974No Hit
CCTACAGGAGTACCTCTCTCTAGAACTGTACGCTGTACCTGCATCAGCAT21600.10056769532830463No Hit
AGGACACAAAGTTGAAAGGAGTCAAGACAAAAATCACTACCTTGGATTTG21570.100428017973682No Hit
ATGCCTTAGACATGCTGGTGCTGTTCAGGGTATGGGGCCTGGGAGGTGAT21560.10038145885547445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)