FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460968
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN73030.2967531475419428No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG56230.2284873269380179No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG55080.22381436898001114No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG52150.2119084847913504No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG51930.21101452761677517No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC49650.20174988053481394No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT48130.19557344914683977No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG47420.19268840553798341No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT46740.18992526518020553No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT46580.1892751145077872No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA44380.1803355427620351No Hit
CGCAAAAAGAAAATATATGAAGAGAAAAACTTACTTGCAGTATCCTGTGC39820.16180624859811263No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT39000.15847422640196868No Hit
CTGAGGGTCTGGAAACTCCTCTCCAAAGCATAGAGTCACCTGGAATCTTG38230.15534537629095543No Hit
GATGAGGGTTTCTGAACATGGTCTCTTTCTTGTGGGCTTCTACAGAGCTG35390.14380520185553No Hit
CTGCCGAAGCCTTGGCGTTCTCAGGTGAGTGCAGGGTTTGCTCCTGGAGG33660.1367754477100068No Hit
CTTATCTCAGCCTCTCCTGCACTCCTTTAGCAGGTTCACAACTACATGAT33040.13425611385438574No Hit
CTCTTCATTCTTTCCCACCAGGCTTGGGCACTGTTTAAAGGAAAGTTCCG32440.131818048832817No Hit
CAAAGTCATTGCTCTTGTTCAAAGCGCACCGCAGGCGCGTCTTCCAGGTG31920.12970505914745742No Hit
GGGACATTGGTACGGGATTTCCGGGTGTGGCTGATCCGGGACGTAGTCCC31900.12962379031340512No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA30560.1241787784319016No Hit
GAGAACGCCAAGGCTTCGGCAGACCTTATGCTTGGCTCTGTGGGGACTCC28970.11771790612474442No Hit
AGACATCATCTGATTTTTATTTGCAAATGCAGGTTGCCAGGTGACAGGAA28820.11710838986935222No Hit
GCCCAAAGATGACAGAGCTGGCAGCAGAGGCTGGCCTCCTGCGGTGCCTC27560.11198845332405785No Hit
GCTGTTTAATAGTGAGCCAGTTGCAGGATATCTCAGTAATGTCTTTTTAA27140.11028180780895973No Hit
CCTGTTTTTACGTTGTGCCATTTCCCTTTTCCCCAAACATGTAGGAGCCA26880.10922531296627995No Hit
CAGGAGAGCCCCTACCTTTTTTGGCTCCCTCAGGAACAATCCTGTACACT26690.10845325904278316No Hit
GAGCAAAATAATATAGTTGTCTGGCTAGCAGAGGTTCTACCTTTAATAAG26600.10808754928954786No Hit
CTGGGCTGCTGTGGTTCTCTGGCCATGTGGGCAGCTGGCCCACACAGCCT26600.10808754928954786No Hit
TTTATTTTTAGCATTGCAGTGTCGAGATGGCTATGAACCCTGTGTAAATG26450.10747803303415567No Hit
GAACAAGAGCAATGACTTTGAGGAACTGGTTGAGCGGAGCCAGCTGGACA26070.10593392518716213No Hit
GTCCATGAGCCACCCCTACACCATGACAACGCCTTACCCTTCGCTCCCAG25510.10365839783369796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)