FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460968
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN71630.29106432915828245No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG56120.22804034835073025No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG55530.22564291774618767No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG51390.20882026909736334No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG49270.20020577268782042No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC48600.19748326674706865No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT46190.1876903722437675No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG46070.18720275923945373No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA45890.18647133973298313No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT45770.18598372672866936No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT44500.18082315576634886No Hit
CGCAAAAAGAAAATATATGAAGAGAAAAACTTACTTGCAGTATCCTGTGC39840.1618875174321649No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT37870.15388253727801418No Hit
CTGAGGGTCTGGAAACTCCTCTCCAAAGCATAGAGTCACCTGGAATCTTG37300.15156637550752386No Hit
GATGAGGGTTTCTGAACATGGTCTCTTTCTTGTGGGCTTCTACAGAGCTG34160.1388071685613141No Hit
CTTATCTCAGCCTCTCCTGCACTCCTTTAGCAGGTTCACAACTACATGAT33740.13710052304621595No Hit
CTGCCGAAGCCTTGGCGTTCTCAGGTGAGTGCAGGGTTTGCTCCTGGAGG32250.13104599490932023No Hit
CAAAGTCATTGCTCTTGTTCAAAGCGCACCGCAGGCGCGTCTTCCAGGTG31730.12893300522396065No Hit
CTCTTCATTCTTTCCCACCAGGCTTGGGCACTGTTTAAAGGAAAGTTCCG30890.12551971419376443No Hit
GGGACATTGGTACGGGATTTCCGGGTGTGGCTGATCCGGGACGTAGTCCC30370.12340672450840483No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA29750.12088739065278378No Hit
AGACATCATCTGATTTTTATTTGCAAATGCAGGTTGCCAGGTGACAGGAA28380.1153204755202018No Hit
GAGAACGCCAAGGCTTCGGCAGACCTTATGCTTGGCTCTGTGGGGACTCC27400.1113383026516395No Hit
GCTGTTTAATAGTGAGCCAGTTGCAGGATATCTCAGTAATGTCTTTTTAA27040.10987546363869828No Hit
GCCCAAAGATGACAGAGCTGGCAGCAGAGGCTGGCCTCCTGCGGTGCCTC26930.10942848505141067No Hit
CCTGTTTTTACGTTGTGCCATTTCCCTTTTCCCCAAACATGTAGGAGCCA26580.10800628045549555No Hit
GAACAAGAGCAATGACTTTGAGGAACTGGTTGAGCGGAGCCAGCTGGACA26130.10617773168931899No Hit
GAGCAAAATAATATAGTTGTCTGGCTAGCAGAGGTTCTACCTTTAATAAG25980.10556821543392682No Hit
CAGGAGAGCCCCTACCTTTTTTGGCTCCCTCAGGAACAATCCTGTACACT25850.1050399680125869No Hit
CTGGGCTGCTGTGGTTCTCTGGCCATGTGGGCAGCTGGCCCACACAGCCT25430.10333332249748879No Hit
TTTATTTTTAGCATTGCAGTGTCGAGATGGCTATGAACCCTGTGTAAATG25270.10268317182507046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)