FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004965383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636670
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN176161.076331820098126No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG39880.2436654915163106No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG35810.21879792505514248No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG29120.1779222445575467No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA27910.17052918425828054No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC26470.1617308315054348No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG25930.15843144922311767No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT25870.1580648511917491No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG22870.1397349496233205No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT22270.1360689693096348No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT19290.11786126708499575No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA16640.10166985403288384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)