FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004965384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357111
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN488133.596831799314868No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG74200.5467496763345076No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG54710.4031357788714409No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT46110.3397658702935869No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG37760.27823811022090306No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA37470.27610121795490566No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC35520.2617324596145783No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG34440.2537743780722432No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG27280.20101524488416936No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG26960.19865729479755156No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG24090.17750942995819796No Hit
GAGGGGACAGATGTGGTCCCTCAGCCTGGGGGGACAAGTGTGGCTCCTC22750.16763551397048584No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT22170.16336172943849103No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT21850.1610037793518732No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC21670.1596774324281507No Hit
GAGGAGCCACACTTGTCCCCCCAGGCTGAGGGACCACATCTGTCCCCTC21640.15945637460753026No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG20850.1536351853311925No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA20220.14899297109816367No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG19430.14317178182182594No Hit
CAACATTTTAATTAAAATGATTTACCTTAACGAAGTCTCTATCAGTCTTCTCTTTCCATTCTTCTCCAAATAC19260.1419191208383102No Hit
GTATTTGGAGAAGAATGGAAAGAGAAGACTGATAGAGACTTCGTTAAGGTAAATCATTTTAATTAAAATGTTG19240.1417717489578966No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG18170.13388735335576823No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA17940.13219257673101167No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT17940.13219257673101167No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG17860.13160308920935723No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC17630.12990831258460067No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG17520.1290977672423258No Hit
CCCGCAGGCGGGAGTAGCAGTGGTTCATGTCGTCCAGCAAGCTCAGCGGCTCCTC17180.12659244527529434No Hit
GAGGAGCCGCTGAGCTTGCTGGACGACATGAACCACTGCTACTCCCGCCTGCGGG17130.12622401557426033No Hit
GGTCAGGTTACGCAGCAAGGCCATGCCAGTTTCTGTCAGCCACACACCTT16980.12511872647115824No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC16870.12430818112888335No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA16870.12430818112888335No Hit
AGAAGGAGGAGCTGCTGTTGGTATAGTGCTGAGGGTTCCAATGTTACCAG16720.12320289202578125No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG16640.12261340450412678No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG16600.12231866074329956No Hit
CCTCCTTAGCCCACTGATGAACGATGGCTCCAACTCTGGTAACATTGGAA16520.1217291732216451No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG16480.12143442946081788No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA16430.12106599975978383No Hit
CAAAACAAAAACATCCCGGGGCCGGGGCGCGCGGCCGGCGCTCACCTGAG16420.12099231381957702No Hit
TGTCATCCACGTCCTGGAGCCAGGAGATGAACTGGCGGGCGTTGAAACGG16410.12091862787937022No Hit
CAGCTTTCTATCACATAGTGAAGGACGACGCATTGTCGTTATCTGCGGTC16390.1207712559989566No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA16380.1206975700587498No Hit
CACAGAGCATGTGGAGGCCTTTATCACCATCCAGGTGGCCCAGATGGTGA16330.12032914035771576No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG16140.11892910749378643No Hit
AACAGAAGCTCATGGCCAGCAGGGGGACCAGAAAGCCGATCACCATCTGG16130.11885542155357963No Hit
CCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAA15980.11775013245047751No Hit
CAAGACCCTGGAGGGCGAGTTCTCGGTCACCATGTGGTCCTCAGGTGAGC15790.1163500995865482No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA15760.11612904176592778No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT15720.11583429800510053No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT15600.11495006672261884No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT15540.11450795108137801No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG15400.11347634791848273No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA15400.11347634791848273No Hit
CCACAGGGTGACGAGAACAAGTCAGTGCGCGACCGTTTCAACGCCCGCCA15360.11318160415765548No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC15140.11156051347310575No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA15030.11074996813083086No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC15010.11060259625041725No Hit
GGCACAAGCTCAGGCCGCAGACGGTGCCGGCCCCGAGGACGACACTGAGG14930.1100131087287628No Hit
CCGGCTGGATGCCGCCAGGAGGGCCTTCGGCTGGGGCGGCGGCACGGGAG14830.10927624932669472No Hit
CTTTCTAGAATTTTAGTCATACCTGTAGACACCAACTGCCTCCTTAGAAG14790.1089815055658675No Hit
GGAGAAGCAACGGCAGGTAAGTTGACACCCTGGAGCTAAGCCCCCTATAT14650.1079499024029722No Hit
CCCCGCGCGGTGAAGGGCGTCAGGTGCAGCTGGCTGGACATCTCGGCGAA14640.1078762164627654No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA14600.10758147270193816No Hit
CCACCTGACCCTCCGCCAGGCCGGCGACGACTTCTCCCGCCGCTACCGCC14540.10713935706069733No Hit
GGGAAGCTTTGGTTGGGCAACACATTGTCAAAGTTAAAATCCAATGTATC14430.10632881171842244No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC14420.10625512577821564No Hit
CCCCAGGAGGCCAAAGACACAGATCAAGGAGTAGGCAATCGGTACAAATA14340.10566563825656118No Hit
GTGGCCCACGCCCTGCATGCCAAAGGGCTGGCTGTTGTAGCTGAGGTCCA14300.10537089449573396No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC14170.10441297727304547No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC14130.10411823351221823No Hit
GAACCAATCAAGCATTGCCCAAGATCGAGGTGAGAGCCTGGGTGTTGGGG14060.1036024319307706No Hit
CCTCACACAACACACCAGTGAAACCTTTGGAAAGAATTTTATCAAGGATT14050.10352874599056379No Hit
GCCCATGCGCTTCCGGCACTTAAAAAAGACTTCTAAGGAGGCAGTTGGTG14030.10338137411015018No Hit
GACCCAGTCCCGGGTGCTCAGTTCCCCCCAGCTGGCACAGCAGGGTCAGG14030.10338137411015018No Hit
GAAGGAAGAGCGGGACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACG13960.10286557252870251No Hit
GGGCAAGCTGGAGTACCTGTACCTGCAGGCCAACCTCATCGAGGTGGTC13900.10242345688746167No Hit
CGCACCCCCTCCCTCCCTGGCGGAGCCCCTGAAGGAGCTGTTCAGGCAGC13870.10220239906684125No Hit
CCATAACAGCTTTTTTCTCTCCTTGAAGTATTTGTCTGAAAGTGGCCACC13770.10146553966477317No Hit
GAGCTGTGCGTAGCGCATGGCTAAGTTGTAGTTGTCGTCCTTCACCTTGA13770.10146553966477317No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA13770.10146553966477317No Hit
TGCAGGAGGTCAGGCAGTTCTCCAGGCTATTTGTACCGATTGCCTACTCC13760.10139185372456637No Hit
CCATTTAGTTGACTATCATCTCGAGATTTCACGTAACGACGATGGTTTTGATAGAAATTAGACAGTCCAT13720.10109710996373915No Hit
CTACTCCAATCATAGGGCTGCCCCAGAGACAGGAGCGATATAGGGGGCTT13650.10058130838229148No Hit
CAGCCTCCTCCTGCTGCTGCTTCAAGATATACTTTTGGCAATCCTCCTCT13630.10043393650187787No Hit
ATGGAGGTCCTTATGACAGAGTGAGGTAAGCATGACCCCAGCTCCTGTCC13580.10006550680084385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)