FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004965385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357111
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN478173.523440602868888No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG74690.5503602874046412No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG53940.3974619614755167No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT44840.3304077558873224No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG39520.2912068356973011No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA37840.2788275977425575No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG34730.25591127033824057No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC33900.2497953373010756No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG26690.19666777441196778No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG26310.1938677086841091No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT23810.17544622363240736No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG22050.16247749815600934No Hit
GAGGAGCCACACTTGTCCCCCCAGGCTGAGGGACCACATCTGTCCCCTC22010.16218275439518212No Hit
GAGGGGACAGATGTGGTCCCTCAGCCTGGGGGGACAAGTGTGGCTCCTC21220.15636156511884436No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC21170.15599313541781035No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT21090.15540364789615588No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA20420.1504666899022998No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG20300.14958245861981814No Hit
CAACATTTTAATTAAAATGATTTACCTTAACGAAGTCTCTATCAGTCTTCTCTTTCCATTCTTCTCCAAATAC18990.13992960045272643No Hit
GTATTTGGAGAAGAATGGAAAGAGAAGACTGATAGAGACTTCGTTAAGGTAAATCATTTTAATTAAAATGTTG18960.139708542632106No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG18370.1353610721599044No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC17870.13167677514956405No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG17870.13167677514956405No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG17810.1312346595083232No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA17730.13064517198666875No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG17300.12747667655777603No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA17290.12740299061756924No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG17250.12710824685674202No Hit
TGTCATCCACGTCCTGGAGCCAGGAGATGAACTGGCGGGCGTTGAAACGG17030.12548715617219225No Hit
GAGGAGCCGCTGAGCTTGCTGGACGACATGAACCACTGCTACTCCCGCCTGCGGG16870.12430818112888335No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT16810.1238660654876425No Hit
GGTCAGGTTACGCAGCAAGGCCATGCCAGTTTCTGTCAGCCACACACCTT16770.12357132172681527No Hit
CCCGCAGGCGGGAGTAGCAGTGGTTCATGTCGTCCAGCAAGCTCAGCGGCTCCTC16750.12342394984640166No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG16700.12305552014536761No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG16670.1228344623247472No Hit
AGAAGGAGGAGCTGCTGTTGGTATAGTGCTGAGGGTTCCAATGTTACCAG16670.1228344623247472No Hit
CAGCTTTCTATCACATAGTGAAGGACGACGCATTGTCGTTATCTGCGGTC16580.12217128886288593No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC16460.12128705758040424No Hit
CAAGACCCTGGAGGGCGAGTTCTCGGTCACCATGTGGTCCTCAGGTGAGC16300.12010808253709535No Hit
AACAGAAGCTCATGGCCAGCAGGGGGACCAGAAAGCCGATCACCATCTGG16240.11966596689585451No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG16210.11944490907523407No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA16200.11937122313502727No Hit
CCTCCTTAGCCCACTGATGAACGATGGCTCCAACTCTGGTAACATTGGAA16150.11900279343399324No Hit
CCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAA16150.11900279343399324No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT16130.11885542155357963No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA16050.11826593403192517No Hit
CACAGAGCATGTGGAGGCCTTTATCACCATCCAGGTGGCCCAGATGGTGA15870.11693958710820265No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT15760.11612904176592778No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA15720.11583429800510053No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA15650.1153184964236529No Hit
CAAAACAAAAACATCCCGGGGCCGGGGCGCGCGGCCGGCGCTCACCTGAG15560.11465532296179162No Hit
CCACAGGGTGACGAGAACAAGTCAGTGCGCGACCGTTTCAACGCCCGCCA15190.11192894317413978No Hit
ATGGAGGTCCTTATGACAGAGTGAGGTAAGCATGACCCCAGCTCCTGTCC15000.11052891031021043No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC14940.1100867946689696No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC14890.10971836496793558No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC14840.10934993526690152No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT14740.10861307586483346No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG14680.10817096022359263No Hit
GGCACAAGCTCAGGCCGCAGACGGTGCCGGCCCCGAGGACGACACTGAGG14670.10809727428338581No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC14630.10780253052255857No Hit
CCGGCTGGATGCCGCCAGGAGGGCCTTCGGCTGGGGCGGCGGCACGGGAG14620.10772884458235177No Hit
CGAAAACCTCGTGGGAGACCTAGAAGTGGCTCTGACCGAAATTCAGCTAT14600.10758147270193816No Hit
CCACCTGACCCTCCGCCAGGCCGGCGACGACTTCTCCCGCCGCTACCGCC14590.10750778676173135No Hit
CTTTCTAGAATTTTAGTCATACCTGTAGACACCAACTGCCTCCTTAGAAG14570.10736041488131774No Hit
GGGAAGCTTTGGTTGGGCAACACATTGTCAAAGTTAAAATCCAATGTATC14520.10699198518028372No Hit
CCCCGCGCGGTGAAGGGCGTCAGGTGCAGCTGGCTGGACATCTCGGCGAA14510.10691829924007688No Hit
CCATAACAGCTTTTTTCTCTCCTTGAAGTATTTGTCTGAAAGTGGCCACC14310.10544458043594077No Hit
CCCCAGGAGGCCAAAGACACAGATCAAGGAGTAGGCAATCGGTACAAATA14250.1050024647946999No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC14230.1048550929142863No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA14070.1036761178709774No Hit
CTACTCCAATCATAGGGCTGCCCCAGAGACAGGAGCGATATAGGGGGCTT14070.1036761178709774No Hit
TGCAGGAGGTCAGGCAGTTCTCCAGGCTATTTGTACCGATTGCCTACTCC14020.10330768816994335No Hit
GGAGAAGCAACGGCAGGTAAGTTGACACCCTGGAGCTAAGCCCCCTATAT14010.10323400222973654No Hit
CCGACAGGGAGGCTCGCTTCCTGTGCTCCTGCCTCTTCCTCACTGGCTTC13980.10301294440911613No Hit
AGGGCACTCAAATGTCTGCCCTAGCTCCCTGCCCCTCCCCAACACCCAGG13970.10293925846890932No Hit
GCCCATGCGCTTCCGGCACTTAAAAAAGACTTCTAAGGAGGCAGTTGGTG13930.1026445147080821No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG13900.10242345688746167No Hit
GTGGCCCACGCCCTGCATGCCAAAGGGCTGGCTGTTGTAGCTGAGGTCCA13850.10205502718642764No Hit
GAGCTGTGCGTAGCGCATGGCTAAGTTGTAGTTGTCGTCCTTCACCTTGA13810.10176028342560042No Hit
GAAGGAAGAGCGGGACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACG13740.10124448184415276No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC13730.10117079590394595No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA13670.10072868026270512No Hit
ACAAAAGCAAGGTGTCACATCCGGAGATAAACATTTATTACAGGGACTGG13630.10043393650187787No Hit
GACCACCTCGATGAGGTTGGCCTGCAGGTACAGGTACTCCAGCTTGCCC13590.10013919274105065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)