FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004965388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529295
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN116170.7596310718337534No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG36850.24096070411529494No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG35770.23389862649129176No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG28290.1849871999843065No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG25290.16537031769540866No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT24130.15778512321036817No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC21410.13999914993510082No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC20440.13365635799502384No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG20150.13176005937376373No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG20000.13077921525931885No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG19430.12705200762442825No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT19220.1256788258642054No Hit
CGAAGTCCGTGTAGACGGTGTCCTTGAGCAGCGCCCCCGACCCGAAGTCG17760.11613194315027514No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT17570.1148895406053116No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA16210.10599655396767792No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA16150.10560421632189997No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA15860.10370791770063985No Hit
CGCCTTGTCTCCTACAGGGAAACCAATGAAAAGCGTGCTGGTGGTGGCTC15770.10311941123197291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)