FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457748
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN193130.7858006597909957No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG59830.24343423328998742No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG49690.20217695223432183No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG44720.18195518824549953No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG44020.17910705247242598No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT39190.15945491563821843No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC33220.13516438625929103No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT32050.13040393075286807No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA30080.12238846293436106No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA29000.11799419631304754No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT27860.11335580376832775No Hit
CCTCGGTGTCCGAGGACCTGGGGCTGGTGCATTTTCGGTTGTTGCTGATC26100.1061947766817428No Hit
CCTGCTGCCAAGAGGGTCAAGTTGGACAGTGTCAGAGTCCTGAGACAGAT25690.10452658287179971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)