FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775015
Sequences flagged as poor quality0
Sequence length35-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN393401.4176499946847134No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG94690.34122338077451836No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG73460.2647192898056407No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC72090.25978237955470507No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG68690.24753019353048542No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG68270.2460166881980818No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT64630.2328996419839172No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG58510.21084570714032178No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG56990.2053682592706706No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT48580.17506211678135075No Hit
GTAGCTGCCCTGGGTGTTATGGCAGAGACCATGGTTCATGCAGGGCTTGG45610.16435947193078235No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA45180.16280993075713104No Hit
TCCTAGGTCAGCGGTGTACCATTGACATTGACGAGTGTATCTCCAAGCCC44310.1596748125685807No Hit
CCTCGGTGTCCGAGGACCTGGGGCTGGTGCATTTTCGGTTGTTGCTGATC43760.15769284129995695No Hit
GAACACACTGGCGGTTGTCAATTTCCAGAAAGACTTTAGAGCTGTGGGAT42440.15293611025525988No Hit
CAGACTCGACTCACCAGAGGCAGGCTGACCAACTCCTGCATTCGACTGTT42150.15189107085907644No Hit
ACAAGTTCAGCAGCCCCAACAAGTACAGATGCAAGTTCAACCTCAACAGT41850.1508099956216453No Hit
GACCTGTCCCAGAAGCTGTATGCCACCATGGAGAAGCACAAGGAGGTAGG41800.15062981641540676No Hit
TCATCTGCAGACGCAGAGTCTGGAGCATGGGCAGGCGGGCCAGTGGCCGG41790.15059378057415906No Hit
CCTGCTGCCAAGAGGGTCAAGTTGGACAGTGTCAGAGTCCTGAGACAGAT41670.1501613504791866No Hit
ACCCCCATCTCTCCTCCTCCGCTTCTCCCTTGGCATCCCACAGCTCTAAA41440.1493325261304894No Hit
CCCTCCTCCCGGCCAGAGGCACGGCTGCAGCACCGCAGCCCACGCCTACC41370.14908027524175546No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT40990.14771091327434266No Hit
GCACGGCATCTTCTTCCGCTCACTCGATGAGACCACGCTCCGGCCACTGG40730.14677398140190231No Hit
AAAGGCCTCCACATGCTCTGTGATGAGAGAGCATCGCATCGCTTGCTCAC40410.14562083448197577No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC39960.14399922162582907No Hit
CCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAA39600.14270193134091166No Hit
CCATTAGCTCTGTTGTATCCCGGTGAGGGCAGCCTCTGGCTTGGGCAGGG39270.14151274857973742No Hit
GCCAGAGCAGGGTTGAGCTTACCTGGAGAGAGGCTGAGTGCAAATTTGGT38530.13884609632740724No Hit
CCAAAGGGAAGGATGGGCTACATCACCCCCTTGCTTCTGCAGTCCAAACA38160.13751277020124217No Hit
GGCCCAGAAACTGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCACC37370.13466593874267346No Hit
CCAGGACCCTGCTGCTGTTGCTGCTGGATTGCCACCTGTCCTAGAAGGTG37140.13383711439397625No Hit
ATGCCTTAGACATGCTGGTGCTGTTCAGGGTATGGGGCCTGGGAGGTGAT36940.13311639756902216No Hit
CCAAAGACTGGCTCGCAGGGCCTCACATGAAAGAGGTCCTACCTGCATCT36910.13300829004527903No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA36610.1319272148078479No Hit
CAAATGGTGGGAACAGACGAGATGCCTCCGGTGGTGGAGACATGGGTGAC36440.1313146055066369No Hit
TACCTGCAGAGCAAGGAATGTGACTTCCAGACCAAATTTGCACTCAGCCT36390.13113442630039837No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG36220.1305218169991874No Hit
GCTCTGGCTTCTGGGTTTCTGCTAGGTTGTCTGGGGGATCCCAAGGTCCC36140.13023353026920576No Hit
CGCCTTGTCTCCTACAGGGAAACCAATGAAAAGCGTGCTGGTGGTGGCTC36000.12972902849173787No Hit
AGGGGTGGGCACACCGGTCGGCTCTGGTCAGGCCTAGGAGATGCAGGTAG36000.12972902849173787No Hit
GAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGGTAA35690.12861191741305902No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA35310.12724255544564625No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC35290.12717048376315082No Hit
CACCTGCTGCTGCCCAGCTTGCAGATACCTTGTTTAGCAAGGGTCTGGGA35010.1261614802082151No Hit
TGGTCTGAGGGAGAGCTGTCTTGTCATGGATTATATCACCTCCCAGGCCC34930.12587319347823345No Hit
CAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAGGACGAGGTGA34860.12562094258949952No Hit
GCTCGTGGTAGAGGTCCAGCTTATTGTGGGACAGGTCTAGCACCTGCAGA34730.12515247665327936No Hit
CCCTTCCCTGGCCCTGGCAGAACCTGAATGGCGTGATGGTGGCAGTGGCG34580.12461193903456377No Hit
CGCCTGTCTCCACCACCTGAGGAGTCACCCATGTCCCCTCCACCTGAAGA34470.12421554478083902No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA33970.12241375271845377No Hit
CGCAGGCAGTCAATGGCTCCCAGTTCCTGCCGCTGACCGGTCTGCAGGTG33640.12122456995727951No Hit
GCACCACTCTGCATTTGCCTAGCCTGCCTTCCTCTAACTGTGCTCTCCCT33440.12050385313232541No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT33350.12017953056109607No Hit
GGTGCAGGGGTTGGTGTAGTCGCAGACAGTGATGAACCTCAGGATGCTGG33230.11974710046612361No Hit
GATCCCCATCAAGTACAAGGCAATGAAGAAAGAGTTCCCCAGCATCCTGA33190.11960295710113278No Hit
GGCAGAGAACCTCACCTGGAAAATGACAAGGAGAGCCACCACCAGCACGC32850.11837773849871082No Hit
GCCACATGGGAAGGGGGTGAGGTGTTCCACGGGCACGATGCGCACGCCAC32620.1175489141500136No Hit
CAAGACAGGCACGGCAATACTCCCCTCCATGTGGCATGTAAAGATGGCAA32370.11664801811882097No Hit
CTCCAGGTGGATGCATGCAGGCTGTGAGAGCCTCTTCACAGAGGACGATG32090.11563901456388523No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG32090.11563901456388523No Hit
CAAACAGTTGTACAATCTCCTGGGAGGTCCATGACCTCTGCTTGCTCAGT31910.11499036942142656No Hit
ACCACCTCATGTTCCTCCTAAGAGAAGTGGCCTTACCTTGTCTTTCACAT31530.11362100745401377No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG31520.11358497161276607No Hit
CCCATCTGAGCCGCCATCTGGCCCATGGAGCTGCCCTGGAGGGGGAGATG31460.11336875656527982No Hit
ACCACGTAGGGCTGGCAGGAGACACAGTCAAAGCCTTCATCGGCTGCCTG31300.11279218310531655No Hit
GGGTGAACTTGGCAGGGCACACCTTGCATTGGTATGGTTTCTCTCCAGAA31200.11243182469283951No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA31130.11217957380410556No Hit
TATCCAAGGACACATGGATGCAGACCACATCTCTAGCCCTCCTTGTGGTTCAGTAGAGCAAGGTCATGGCAACA31120.11214353796285786No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC31100.11207146628036246No Hit
GCATACCTTGTTGGAGATGTCCAAATTGCAGTTTGCTTCACAGAGGGCCA31070.11196335875661932No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA31050.11189128707412392No Hit
GGCAGTCGCGCAGGGGAAGTGGTAAAGCCGTGGACATCCAGGTGAGCGGC30870.11124264193166522No Hit
AGCCAAACTGCCAGGGGTCTGTCCAGGCTCTGGCTGTGAACCCGGAGCAT30810.111026426884179No Hit
CAAGGACCCGGAGGCTACCCACCGGCCATGCAGCAGCAGCAGCGCATGCA30490.10987327996425246No Hit
GAGCTGTGCGTAGCGCATGGCTAAGTTGTAGTTGTCGTCCTTCACCTTGA30470.10980120828175703No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC30400.1095489573930231No Hit
AGTAACGTACTGTTTGCTGATGAAGCCAATGATCAGCCTGGCCGGGATGA30240.10897238393305982No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG30220.10890031225056442No Hit
GAAGGAAGAGCGGGACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACG30170.10872013304432587No Hit
TGTTGCCATGACCTTGCTCTACTGAACCACAAGGAGGGCTAGAGATGTGGTCTGCATCCATGTGTCCTTGGATA30130.10857598967933507No Hit
CTGGCCCAGGCTGGGGGGCATCTGCAGGTGAGGCCTGGAAGTAGGGAGAG30100.10846788215559196No Hit
AACAGAAGCTCATGGCCAGCAGGGGGACCAGAAAGCCGATCACCATCTGG30040.10825166710810571No Hit
GGTCAGGTTACGCAGCAAGGCCATGCCAGTTTCTGTCAGCCACACACCTT30030.10821563126685801No Hit
CACAGAGCATGTGGAGGCCTTTATCACCATCCAGGTGGCCCAGATGGTGA29900.10774716533063786No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG29870.10763905780689473No Hit
CCCGAAAAGCCGTCCAAGGGCTCCGGCTACCGCTGCATTCGCATCAACCA29800.10738680691816081No Hit
GGCCTTCTTGCTGACAGGTATCATTTCTGTGAGAAGTGTTTCACAGAGAT29390.10590933742700491No Hit
CCCGTGGCCTCGAGGGCAAAGCCGATACACCAGGTGAGGGCTGCCGGGGT29220.10529672812579392No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA28900.10414358120586736No Hit
AGCAGCACCAGCAATCTCAAGACCCACCTGCGACTCCATTCTGGAGAGAA28840.10392736615838112No Hit
GCCCACAGCTGGTACAGAAGAACAGGTCACACAACTCCCCTGGCCCCTCA28780.1037111511108949No Hit
ACACCAGTCACCTCCATACTCACTGCCCATGCCCCATCCTCTCCTTCATC28770.10367511526964718No Hit
AGAAAGTGGAGCATGTCATCAAAGATAGGCTGCAAGTCACAGGGGTCCAG28750.10360304358715178No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT28690.10338682853966556No Hit
GACCCCATTGATCGACGGCCATGGACACAGCAGCACCTTGAAGCTGCAGA28490.10266611171471146No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT28490.10266611171471146No Hit
CCACCTCCTGCTCTGCCTGAGGAGGGGTGAGAGCCAGCGATGGTGTCCTA28290.10194539488975736No Hit
GGGGCTGTGAAGGGTCGTCACCCAGGGTCACATTCTCGCCCTGGATCTCT28290.10194539488975736No Hit
TTCACCCTGGCAGCGCTGCTCCCACTGCACCAGGCTCGGTGCCTCCATCC27970.10079224796983079No Hit
CCCTCTCTTCTCTGCAGAAAAGAAGGACACAGTGTTGCGGCAGGTACGCC27840.10032378203361063No Hit
GGAGACATTGTAGGTATAGCTGTGCTGAGTGGGTGGCTCTGGGGCGGGGC27820.10025171035111521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)