FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004965395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221136
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1375314.269642759573021No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC126590.3929979982217453No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG124040.3850815364517363No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG102750.3189868419091898No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG75120.23320965026003249No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG64400.19992946587787663No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG61510.19095747587186632No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT55920.17360334987408169No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA54690.16978482125560673No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT50050.15537996532900195No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA49990.15519369564029584No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG46750.14513513245016665No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT35390.1098680713884791No Hit
GAGGAGCCACACTTGTCCCCCCAGGCTGAGGGACCACATCTGTCCCCTC34690.1076949250202413No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT33910.10527341906706206No Hit
GAGGGGACAGATGTGGTCCCTCAGCCTGGGGGGACAAGTGTGGCTCCTC33550.10415580093482547No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC32920.10219996920341147No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT32910.1021689242552938No Hit
GCATCTTCCGGCCACACTCCTTGCAATCAACGAAACTAGGAGGCAAAGAA32770.10173429498164623No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG32650.10136175560423402No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG32370.10049249705693891No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)