FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425828
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN397531.6387394324741904No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG135580.5589019501794851No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG127470.5254700663031344No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG97240.40085282221163254No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT90160.37166691125669254No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT70900.2922713399301187No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA69190.28522220041981544No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG57560.23727980714213867No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA56660.23356973371566328No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG48940.20174554832411862No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT47660.19646899945090912No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC44510.1834837424582452No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG43980.1812989214404319No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG38980.16068740240445736No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT38900.16035761809988178No Hit
AGCAGCACCAGCAATCTCAAGACCCACCTGCGACTCCATTCTGGAGAGAA37270.15363826289415408No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG36950.1523191256758517No Hit
ACACGGATGGCTTTGTGCCTTTTAGAATTCTGAGCTTGCACCAAGGTTTT36450.15025797377225425No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC36170.14910372870623967No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA36160.14906250566816773No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT36010.1484441600970885No Hit
CTCTGAAATCACCTGTTAACGATGTTATTGAGCCGGCTGGCTTGTGGGAT35420.1460120008508435No Hit
GGGTGAACTTGGCAGGGCACACCTTGCATTGGTATGGTTTCTCTCCAGAA35140.14485775578482893No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA34550.14242559653858394No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT34520.1423019274243681No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC34460.14205458919593641No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA34440.14197214311979248No Hit
CCTCACACAACACACCAGTGAAACCTTTGGAAAGAATTTTATCAAGGATT34420.1418896970436486No Hit
CAGTAAGACCCGAGACTCCATGGTGCGGATCAGCTCAGTCCATCGTCTCC34350.14160113577714495No Hit
TCGATTTTTATTTTTATTTTTAAAGAGGGAGACGATGGACTGAGCTGATC34290.14135379754871324No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT34230.14110645932028157No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG34210.14102401324413769No Hit
GAGCTGTGCGTAGCGCATGGCTAAGTTGTAGTTGTCGTCCTTCACCTTGA34110.1406117828634182No Hit
GAACTTGTATTTCTTCCAGTTGCAGGCTTTGGGGTCAGTGGGGCTCTTGG34090.14052933678727428No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG33930.1398697681781231No Hit
GGTGTGGAATATCTGCCTTGGTCTTTGAGAATCCTTGATAAAATTCTTTC33780.13925142260704387No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA33770.13921019956897193No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC33740.13908653045475605No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG33410.13772617019838174No Hit
GACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCAT33380.1376025010841659No Hit
TGTCATCCACGTCCTGGAGCCAGGAGATGAACTGGCGGGCGTTGAAACGG33050.13624214082779157No Hit
CCACCTGACCCTCCGCCAGGCCGGCGACGACTTCTCCCGCCGCTACCGCC33010.13607724867550378No Hit
CCCCGCGCGGTGAAGGGCGTCAGGTGCAGCTGGCTGGACATCTCGGCGAA32650.1345932193049136No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC32640.13455199626684167No Hit
CCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAA32600.13438710411455387No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA32570.13426343500033802No Hit
GATGTTCATGATATTTTGTTACACGTTCATTGTCAAAACCTTGGTGCAAG32430.13368631246733076No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG32100.13232595221095642No Hit
GGTCAGGTTACGCAGCAAGGCCATGCCAGTTTCTGTCAGCCACACACCTT31900.13150149144951745No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC31850.13129537625915771No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG31690.1306358076500065No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC31680.13059458461193454No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC31390.12939911650784805No Hit
ACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGT31360.1292754473936322No Hit
GTTTTCTCTGCAGTCTTTAAACCAGAGGAGTTACGCCAGGCCCTCATGCC31260.1288632170129127No Hit
CTGTAGGAAGAGACACCTCTGTTTCTTGCTGCCCGGGAGGGGAGCTATGA31160.12845098663219323No Hit
CAGCCTCCTCCTGCTGCTGCTTCAAGATATACTTTTGGCAATCCTCCTCT31100.12820364840376153No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG31100.12820364840376153No Hit
GAAGGAAGAGCGGGACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACG31090.12816242536568956No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG31050.1279975332134018No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA30910.1274204106803945No Hit
CAAGACCCTGGAGGGCGAGTTCTCGGTCACCATGTGGTCCTCAGGTGAGC30910.1274204106803945No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC30890.1273379646042506No Hit
CCACAGGGTGACGAGAACAAGTCAGTGCGCGACCGTTTCAACGCCCGCCA30820.12704940333774695No Hit
CGCAGCAACAGCCGACGCCTGTGCACGCCCAGCCTCCTGGCACACCGGTA30810.127008180299675No Hit
GGAACAGACAAACTATCGACTGAAGTTGTGTGTGTCTGACCGCGATGTCC30560.12597760434787628No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA30560.12597760434787628No Hit
GCATTTTAAGACTCTTACCATGTCCTTCATCACACAATCTACATCTTCTG30460.12556537396715678No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA30330.12502947447222143No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA30120.12416379067271052No Hit
CATCAACACCATCCAACTGTGAGATTTTTGGAAGATCACACTGTTCCTCC30050.12387522940620686No Hit
CAGCTTTCTATCACATAGTGAAGGACGACGCATTGTCGTTATCTGCGGTC30030.12379278333006297No Hit
CTGCCGAAGCCTTGGCGTTCTCAGGTGAGTGCAGGGTTTGCTCCTGGAGG30000.12366911421584713No Hit
ATCCACACACTGCCCATTGTTCACACAAGGGTTGCTCTGACATTCATTTA29610.12206141573104111No Hit
CAAAACAAAAACATCCCGGGGCCGGGGCGCGCGGCCGGCGCTCACCTGAG29460.12144307015996188No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG29440.12136062408381798No Hit
AAGAATGCCTGCATCCTCCAGGCTTCTGGCTCCCCTCCAGCCAAGAGCCC29390.12115450889345825No Hit
GGAGAGGAACGACTGGCATCCCATAATTTATTTCGGGAGGAGGAACAGTG29290.12074227851273875No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC28970.11942314129443639No Hit
TATGGGAAAATTATCTTCTAAGAGTCTTGGCCCAAAAACAGCAGAGACAG28860.11896968787564494No Hit
GCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAATT28540.11765055065734256No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC28450.11727954331469503No Hit
ACGCCTTCATCGGCGTCAAGTTCCGCAACGATCTCTTCAAGCTCTTCAAG28350.11686731293397554No Hit
CCCTGACTCTGTCCCACTGCCCAGGTACATTAACCATTCCTGTGCCCCTA28180.11616652128675241No Hit
GAAGGTGCCGGAAACGGGTGCCACAGATTTCACAGGGATAGGGCTTCTCA28170.11612529824868045No Hit
GCCGACAGGAAGACCACTGCCGGAGCTGCTCCTGGCTGAGGCAGCCCAGG28010.11546572963952928No Hit
CCCGCAGCAGGGCGTGGAGGGGGCTGGGGAGGAAATCCTTGGGAGCCCCG28000.11542450660145731No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG27980.11534206052531341No Hit
ATGTCGAAAAGTGTTTCTGTCATCCAAATACTCCACACGCAAATTTCCTT27840.11476493799230612No Hit
CACCCACAGGCGGATGTCCGCGTCGCCCTCTGTGCTTTGCTGGTGGATCC27790.11455882280194639No Hit
CACGGAGCGAGAGAGCACAGCGAGGGCCACAGAGGGTGCAGAGCGGGAGA27790.11455882280194639No Hit
GATACTAGAGCCGCTGCCTCCTCGTAGAAGCTCCGACAGTTGTTTATGTT27720.11427026153544273No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA27710.11422903849737079No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC27550.1135694698882196No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC27460.11319846254557207No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT27340.1127037860887087No Hit
GAACTTAGGCCTCCCTGACCTCGTGGCAAAGTACAACACCAATAACGGGG27230.11225033266991724No Hit
TTTGTCCTCTTTGTCAAATGTCACGACTTCGGCCACACAGTTAGGGGCAC27180.11204421747955748No Hit
GAACTTAATTTTACAGTTCAGAGTAAGGAACAAGAAAAAGACTCAGAAAT26990.11126097975619047No Hit
TCGATGAGCTCACTAGCAATAGACCAGACACAGGTGCCAGGCAGGACATC26910.11093119545161487No Hit
CAAATGTTATCTGTGAAAAGGCAGCAACAGCAGCAACATTCACCAGCACC26900.11088997241354291No Hit
CATGCGGTGCTTGTACATTTCACAGTCCTTTCCCAGGGTCGAGCACTTGA26590.10961205823331249No Hit
GACAGAGAGAGTAGTTCTCAAACCACTCCGAGTTGGGATTCAGTTCTGCT26590.10961205823331249No Hit
GAACAAGAGCAATGACTTTGAGGAACTGGTTGAGCGGAGCCAGCTGGACA26390.10878759747187353No Hit
CGTTCAGGTGGCCCACCTCCGTGCCCATGTGCTTATCCACACTGGTGAGA26370.10870515139572963No Hit
GGACAGCAACAAGGGTCAAGGTAATCACAATCACCATCTACCTGCTGTGT26330.10854025924344182No Hit
GGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCT26130.10771579848200286No Hit
GGAACACCTCACCCCCTTCCCATGTGGCATCTTTCACAAGGTCCAGGTGA26000.1071798989870675No Hit
CTTTCAGCCAATAGCAATCCTTGTGAGCATGCAGGAAAATGTGTGAACAC25890.10672644556827607No Hit
GGCATCTAGATCAGTTACTCGGTTGCGAATCAGAATCTAGAAGAGGAGAAAGTACAGAAAAGAGAGTCACCTGG25850.10656155341598827No Hit
AAGGAAATACTACCTTTTCTTAAACGGAAGATGAATGTCAGTGCTACACC25720.10602565392105294No Hit
CGAGACACAAAGCATGGATGAGATGTTGAGTACTGGCAGTCCTCTCCTGT25710.105984430882981No Hit
CAGAACCAATGGAAGTGGATGAAAAGAAACCTGAAGTGAAAGTAGAAGTT25670.10581953873069318No Hit
ATCGCCTCTATGTGTCTGTGAAGGAGATTGGTGGATTGACTCAGGTGAGT25560.10536608531190175No Hit
AAAGAGGTAGGCACTCCCAGGGAGGCTGCTTTACTCTGTCTCTTCCCCAC25480.10503630100732615No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA25390.10466529366467861No Hit
GGACTTTCAGGTGAATCCCTGACTCCAGAACGCACTCAGCTCCTCTATCT25330.10441795543624692No Hit
GAGAGGCTGTGCCGTTACTGCTACTCTCTTCTTCCTCTTTAACTTCTACT25260.10412939416974328No Hit
CACACCCCAATGGCGGGATAGCAGCCCCTGTGTGTGAGCACCCCTCCATG25130.10359349467480793No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT25100.10346982556059209No Hit
CCAGGTGACTCTCTTTTCTGTACTTTCTCCTCTTCTAGATTCTGATTCGCAACCGAGTAACTGATCTAGATGCC25030.10318126429408846No Hit
GGCCTGGAGGCTATCCAGCGTGAGTCTCTCCTACCCTCCCGCTCTGGTCC25000.10305759517987262No Hit
AACCCTGGGGTTGAAGACTGGGCTTGTCCCACCATGGGGCAAGGGCCTGA24920.10272781087529702No Hit
ACCCAGTCTGTCTGAGATAAGCAATCCCGAAAACATGGAAAATCTTTTGG24920.10272781087529702No Hit
AATGATGTTGGTGATGAGAGAAGGTTGAGATTATCCAAAAGATTTTCCAT24910.10268658783722506No Hit
GTCCCCAGATGCCCTGCCTGAATGGAGGGACTTGTGCTGTGGCCAGTAAC24890.10260414176108117No Hit
GACTGCTTCCTACATCGTAAGTGCAATTATTGTACGTTTTCATTTTCTAT24840.10239802657072142No Hit
CCCCCACTGTGTAAGTGGAATTATTTTTCACTGCAGCATTTCTGAGTCTT24760.10206824226614583No Hit
AGAGAGGTATAGAGGGGATAACAGATGCCGAGTACCTGGCATGGAGGGGT24630.1015323427712105No Hit
GAACCCAAATTATACCCCTTGCCAAAAATAAAAATCTTACCCCGCTCATT24600.10140867365699466No Hit
AGGACATCTCTGATTGGAGGGGGGACTCTTGCTCATAGGAGATGATGGTC24430.1007078820097715No Hit
GAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGCCAAGCGCAAAGCGTCTG24360.10041932074326787No Hit
CCGGGATCTGGTCACGCAGGGCAAAAAAGCTCCGTTTTAGCTCGTTCCTC24310.10021320555290814No Hit
GCCCACTCCCTTCCCTTAGGCGTGCCGGGTGTGCCGGGCCTGTGGGGCGG24290.10013075947676421No Hit
AGAAGGAGGAGCTGCTGTTGGTATAGTGCTGAGGGTTCCAATGTTACCAG24270.10004831340062031No Hit
GAGAGCGGCCAAAGATACGCCGCTGCGCTCCTTGGAGGCGGCAACAGCTT24260.10000709036254837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)