FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425828
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN390521.6098420827857538No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG135330.5578713742276864No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG116310.47946515581483934No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG98770.40715994703664066No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT87780.36185582819556866No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT76780.31651048631642476No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA72210.297671557917544No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG57700.237856929675146No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA57640.23760959144671426No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG48920.20166310224797473No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT48680.20067374933424795No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC44130.18191726701151112No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG43240.17824841662310767No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT40510.16699452722946556No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG38820.16002783379530616No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG37270.15363826289415408No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC36960.15236034871392365No Hit
AGCAGCACCAGCAATCTCAAGACCCACCTGCGACTCCATTCTGGAGAGAA36460.1502991968103262No Hit
GGGTGAACTTGGCAGGGCACACCTTGCATTGGTATGGTTTCTCTCCAGAA36040.14856782921130435No Hit
GACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCAT36040.14856782921130435No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA35880.14790826060215317No Hit
ACACGGATGGCTTTGTGCCTTTTAGAATTCTGAGCTTGCACCAAGGTTTT35570.14663034642192274No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG35180.14502264793711672No Hit
CCTCACACAACACACCAGTGAAACCTTTGGAAAGAATTTTATCAAGGATT35080.14461041755639722No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA34990.1442394102137497No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA34750.14325005730002294No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT34630.14275538084315953No Hit
GAAGGAAGAGCGGGACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACG34500.1422194813482242No Hit
TCGATTTTTATTTTTATTTTTAAAGAGGGAGACGATGGACTGAGCTGATC34390.14176602792943274No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG34280.1413125745106413No Hit
GATGTTCATGATATTTTGTTACACGTTCATTGTCAAAACCTTGGTGCAAG34260.14123012843449742No Hit
GTTTTCTCTGCAGTCTTTAAACCAGAGGAGTTACGCCAGGCCCTCATGCC33850.1395399838735475No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC33820.13941631475933167No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA33810.13937509172125973No Hit
TGTCATCCACGTCCTGGAGCCAGGAGATGAACTGGCGGGCGTTGAAACGG33770.13921019956897193No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG33670.13879796918825243No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC33490.13805595450295735No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT33240.13702537855115862No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG33210.13690170943694277No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA32990.1359948025993599No Hit
CAGTAAGACCCGAGACTCCATGGTGCGGATCAGCTCAGTCCATCGTCTCC32830.13533523399020872No Hit
CTCTGAAATCACCTGTTAACGATGTTATTGAGCCGGCTGGCTTGTGGGAT32810.1352527879140648No Hit
GGTGTGGAATATCTGCCTTGGTCTTTGAGAATCCTTGATAAAATTCTTTC32770.13508789576177702No Hit
CCACCTGACCCTCCGCCAGGCCGGCGACGACTTCTCCCGCCGCTACCGCC32760.13504667272370505No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC32620.13446955019069778No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG32600.13438710411455387No Hit
AAGAATGCCTGCATCCTCCAGGCTTCTGGCTCCCCTCCAGCCAAGAGCCC32540.1341397658861222No Hit
CCCCGCGCGGTGAAGGGCGTCAGGTGCAGCTGGCTGGACATCTCGGCGAA32240.1329030747439637No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC32150.13253206740131618No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC32040.13207861398252474No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT31970.13179005271602107No Hit
GCATTTTAAGACTCTTACCATGTCCTTCATCACACAATCTACATCTTCTG31960.13174882967794913No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG31960.13174882967794913No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC31900.13150149144951745No Hit
CCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAA31690.1306358076500065No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA31670.1305533615738626No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA31600.13026480030735899No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA31220.1286983248606249No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC31200.12861587878448102No Hit
CCACAGGGTGACGAGAACAAGTCAGTGCGCGACCGTTTCAACGCCCGCCA31030.12791508713725788No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG30890.1273379646042506No Hit
GAGCTGTGCGTAGCGCATGGCTAAGTTGTAGTTGTCGTCCTTCACCTTGA30890.1273379646042506No Hit
ACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGT30710.1265959499189555No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC30560.12597760434787628No Hit
CGCAGCAACAGCCGACGCCTGTGCACGCCCAGCCTCCTGGCACACCGGTA30480.1256478200433007No Hit
GAACTTGTATTTCTTCCAGTTGCAGGCTTTGGGGTCAGTGGGGCTCTTGG30470.12560659700522872No Hit
GGAGAGGAACGACTGGCATCCCATAATTTATTTCGGGAGGAGGAACAGTG30440.12548292789101287No Hit
CAAAACAAAAACATCCCGGGGCCGGGGCGCGCGGCCGGCGCTCACCTGAG30370.12519436662450925No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG30220.12457602105343No Hit
GGTCAGGTTACGCAGCAAGGCCATGCCAGTTTCTGTCAGCCACACACCTT30140.1242462367488544No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA29890.12321566079705568No Hit
ATCCACACACTGCCCATTGTTCACACAAGGGTTGCTCTGACATTCATTTA29780.12276220737826424No Hit
CAAGACCCTGGAGGGCGAGTTCTCGGTCACCATGTGGTCCTCAGGTGAGC29760.12267976130212034No Hit
CTGTAGGAAGAGACACCTCTGTTTCTTGCTGCCCGGGAGGGGAGCTATGA29680.12234997699754477No Hit
TATGGGAAAATTATCTTCTAAGAGTCTTGGCCCAAAAACAGCAGAGACAG29660.12226753092140087No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA29490.12156673927417772No Hit
GCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAATT29270.12065983243659485No Hit
CAGCCTCCTCCTGCTGCTGCTTCAAGATATACTTTTGGCAATCCTCCTCT29190.12033004813201925No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG29140.12012393294165952No Hit
GGAACAGACAAACTATCGACTGAAGTTGTGTGTGTCTGACCGCGATGTCC28820.11880479572335713No Hit
CATCAACACCATCCAACTGTGAGATTTTTGGAAGATCACACTGTTCCTCC28770.1185986805329974No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC28630.1180215579999901No Hit
GAACTTAGGCCTCCCTGACCTCGTGGCAAAGTACAACACCAATAACGGGG28630.1180215579999901No Hit
TCGATGAGCTCACTAGCAATAGACCAGACACAGGTGCCAGGCAGGACATC28530.1176093276192706No Hit
CGTTCAGGTGGCCCACCTCCGTGCCCATGTGCTTATCCACACTGGTGAGA28320.11674364381975968No Hit
GCCGACAGGAAGACCACTGCCGGAGCTGCTCCTGGCTGAGGCAGCCCAGG28160.11608407521060851No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC28100.11583673698217681No Hit
CAGCTTTCTATCACATAGTGAAGGACGACGCATTGTCGTTATCTGCGGTC27970.11530083748724146No Hit
ACGCCTTCATCGGCGTCAAGTTCCGCAACGATCTCTTCAAGCTCTTCAAG27950.11521839141109758No Hit
TTTGTCCTCTTTGTCAAATGTCACGACTTCGGCCACACAGTTAGGGGCAC27950.11521839141109758No Hit
CCCTGACTCTGTCCCACTGCCCAGGTACATTAACCATTCCTGTGCCCCTA27890.11497105318266589No Hit
AAACAGTGGGAACCTTGTTCCAGATGCTGCTTCCAAACATAAACAACTGT27670.114064146345083No Hit
GGCCTGGAGGCTATCCAGCGTGAGTCTCTCCTACCCTCCCGCTCTGGTCC27570.1136519159643635No Hit
CTGCCGAAGCCTTGGCGTTCTCAGGTGAGTGCAGGGTTTGCTCCTGGAGG27480.11328090862171597No Hit
GGAACACCTCACCCCCTTCCCATGTGGCATCTTTCACAAGGTCCAGGTGA27470.11323968558364403No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT27370.11282745520292453No Hit
ACCCAGTCTGTCTGAGATAAGCAATCCCGAAAACATGGAAAATCTTTTGG27260.11237400178413309No Hit
CCCCCACTGTGTAAGTGGAATTATTTTTCACTGCAGCATTTCTGAGTCTT27200.1121266635557014No Hit
ATGTCGAAAAGTGTTTCTGTCATCCAAATACTCCACACGCAAATTTCCTT27060.11154954102269411No Hit
AAAGAGGTAGGCACTCCCAGGGAGGCTGCTTTACTCTGTCTCTTCCCCAC26970.11117853368004657No Hit
CCCGCAGCAGGGCGTGGAGGGGGCTGGGGAGGAAATCCTTGGGAGCCCCG26940.11105486456583072No Hit
GTCCCCAGATGCCCTGCCTGAATGGAGGGACTTGTGCTGTGGCCAGTAAC26460.10907615873837716No Hit
CACCCACAGGCGGATGTCCGCGTCGCCCTCTGTGCTTTGCTGGTGGATCC26210.10804558278657843No Hit
CGAGACACAAAGCATGGATGAGATGTTGAGTACTGGCAGTCCTCTCCTGT26150.10779824455814674No Hit
AAGGAAATACTACCTTTTCTTAAACGGAAGATGAATGTCAGTGCTACACC26110.10763335240585896No Hit
CAGGAGAGCCCCTACCTTTTTTGGCTCCCTCAGGAACAATCCTGTACACT26100.107592129367787No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC26070.10746846025357114No Hit
GAAGGTGCCGGAAACGGGTGCCACAGATTTCACAGGGATAGGGCTTCTCA26040.1073447911393553No Hit
CCAGGTGACTCTCTTTTCTGTACTTTCTCCTCTTCTAGATTCTGATTCGCAACCGAGTAACTGATCTAGATGCC25660.10577831569262124No Hit
GCAGGGCCGGGCGCAGAGTGCCATGGGCGGCCTCTCTTATACACAGCAGG25650.10573709265454928No Hit
GACAGAGAGAGTAGTTCTCAAACCACTCCGAGTTGGGATTCAGTTCTGCT25650.10573709265454928No Hit
CAGAACCAATGGAAGTGGATGAAAAGAAACCTGAAGTGAAAGTAGAAGTT25640.10569586961647734No Hit
GGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCT25630.1056546465784054No Hit
GAGAGGCTGTGCCGTTACTGCTACTCTCTTCTTCCTCTTTAACTTCTACT25620.10561342354033344No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA25580.10544853138804565No Hit
ACTACAAGCCAACTGGCTTAACACAGGTCAATAAGAAACTATCACATGGG25520.10520119315961395No Hit
CACGGAGCGAGAGAGCACAGCGAGGGCCACAGAGGGTGCAGAGCGGGAGA25520.10520119315961395No Hit
CACACCCCAATGGCGGGATAGCAGCCCCTGTGTGTGAGCACCCCTCCATG25500.10511874708347005No Hit
CAAATGTTATCTGTGAAAAGGCAGCAACAGCAGCAACATTCACCAGCACC25400.10470651670275055No Hit
TCACAGTACCTGGAGGAGAAGGAGGACCTGGAGCTCAAGTGCTCGACCCT25310.10433550936010302No Hit
GCCCACTCCCTTCCCTTAGGCGTGCCGGGTGTGCCGGGCCTGTGGGGCGG25300.10429428632203108No Hit
CATGCGGTGCTTGTACATTTCACAGTCCTTTCCCAGGGTCGAGCACTTGA25190.10384083290323964No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT25190.10384083290323964No Hit
GGCATCTAGATCAGTTACTCGGTTGCGAATCAGAATCTAGAAGAGGAGAAAGTACAGAAAAGAGAGTCACCTGG25170.10375838682709575No Hit
CGAGACACAAAGCATGGATGAGGTGTCGAGTACTGGCAGTCCTCTCCTGT25170.10375838682709575No Hit
GAACTTAATTTTACAGTTCAGAGTAAGGAACAAGAAAAAGACTCAGAAAT25000.10305759517987262No Hit
GGACTTTCAGGTGAATCCCTGACTCCAGAACGCACTCAGCTCCTCTATCT24970.10293392606565675No Hit
GGGGCTGCTGAACTTGTACTTGTACCTGCTGTGGTGGGGGTGCTGGTGAA24910.10268658783722506No Hit
CTTTCAGCCAATAGCAATCCTTGTGAGCATGCAGGAAAATGTGTGAACAC24880.10256291872300922No Hit
AGAGAGGTATAGAGGGGATAACAGATGCCGAGTACCTGGCATGGAGGGGT24870.10252169568493727No Hit
GTACTTGATGGGGATCAGTCGCTTCTGATGGGCACCTGCAAGCCAAGGGG24870.10252169568493727No Hit
ATCGCCTCTATGTGTCTGTGAAGGAGATTGGTGGATTGACTCAGGTGAGT24850.10243924960879336No Hit
GGACAGCAACAAGGGTCAAGGTAATCACAATCACCATCTACCTGCTGTGT24800.10223313441843362No Hit
CTCACAACGAGGTCCTGCATAACCCTTCAGACACTCACAGTGGAAGGCGC24720.10190335011385802No Hit
ACCCCCAGCAGAATAATAAACAGAATGATGACAACAGCAACAGCAAGGAG24600.10140867365699466No Hit
GCCACATGGAGGGGAGTATTGCCGTGCCTGTCTTGATAATCGACGAAACA24590.1013674506189227No Hit
CCCCACCAGGCTGGCTTCCTGTCAGGCGAGCTGGCCCTCAACAGCCCCGG24510.1010376663143471No Hit
CCGACAGGGAGGCTCGCTTCCTGTGCTCCTGCCTCTTCCTCACTGGCTTC24400.10058421289555566No Hit
GAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGCCAAGCGCAAAGCGTCTG24350.10037809770519592No Hit
GAACAAGAGCAATGACTTTGAGGAACTGGTTGAGCGGAGCCAGCTGGACA24340.10033687466712397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)