FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2598399
Sequences flagged as poor quality0
Sequence length35-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1274724.905790065344083No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC79290.305149440097537No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG77970.3000693888813843No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG75190.2893704931382748No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA66590.2562731897603101No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG64570.248499171990137No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC59050.22725532144986205No Hit
CCCGAAAAGCCGTCCAAGGGCTCCGGCTACCGCTGCATTCGCATCAACCA58110.2236377092201775No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA57230.220251008409409No Hit
CGAAGTCCGTGTAGACGGTGTCCTTGAGCAGCGCCCCCGACCCGAAGTCG57180.22005858222697902No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG55460.21343912155138606No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG54770.21078364023385168No Hit
CAAGGACGAAAACATCCTTATCGACCTCAATCGCGGCGAGCTCAAGCTCA53010.20401023861231476No Hit
CCCCAGGCACTGATACTCACCTGACTGAGGTTGAAGGTCATGATGCTGTT52920.20366387148394066No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG52310.2013162720582944No Hit
CCTCGGTGTCCGAGGACCTGGGGCTGGTGCATTTTCGGTTGTTGCTGATC51450.1980065417204979No Hit
CCAAAGGGAAGGATGGGCTACATCACCCCCTTGCTTCTGCAGTCCAAACA50530.1944658999637854No Hit
CCTGCTGCCAAGAGGGTCAAGTTGGACAGTGTCAGAGTCCTGAGACAGAT49450.1903094944232968No Hit
GACCTGTCCCAGAAGCTGTATGCCACCATGGAGAAGCACAAGGAGGTAGG48470.18653794124766826No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG48120.18519095797065807No Hit
CCCTCCTCCCGGCCAGAGGCACGGCTGCAGCACCGCAGCCCACGCCTACC47870.18422882705850796No Hit
CCATTAGCTCTGTTGTATCCCGGTGAGGGCAGCCTCTGGCTTGGGCAGGG47350.18222759476123568No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG46820.1801878772274774No Hit
AAAGGCCTCCACATGCTCTGTGATGAGAGAGCATCGCATCGCTTGCTCAC46760.17995696580856135No Hit
CCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAA46620.17941817249775727No Hit
GCTCGTGGTAGAGGTCCAGCTTATTGTGGGACAGGTCTAGCACCTGCAGA45480.17503085553835265No Hit
CGCAGGCAGTCAATGGCTCCCAGTTCCTGCCGCTGACCGGTCTGCAGGTG45280.17426115080863255No Hit
CAAACAGTTGTACAATCTCCTGGGAGGTCCATGACCTCTGCTTGCTCAGT44710.17206749232893023No Hit
GTTGAGGCTGCCCACATCCTGGTAGGTGCCCTGGAGGCCCCGGGAGATGT43780.16848836533573172No Hit
CGCCTTGTCTCCTACAGGGAAACCAATGAAAAGCGTGCTGGTGGTGGCTC41730.16059889185610063No Hit
CAAATGGTGGGAACAGACGAGATGCCTCCGGTGGTGGAGACATGGGTGAC41400.15932887905206244No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA41120.1582512924304543No Hit
GCCAGAGCAGGGTTGAGCTTACCTGGAGAGAGGCTGAGTGCAAATTTGGT40700.15663491249804207No Hit
GTAGCTGCCCTGGGTGTTATGGCAGAGACCATGGTTCATGCAGGGCTTGG40680.15655794202507003No Hit
GCTCTGGCTTCTGGGTTTCTGCTAGGTTGTCTGGGGGATCCCAAGGTCCC40600.15625006013318202No Hit
CAGACTCGACTCACCAGAGGCAGGCTGACCAACTCCTGCATTCGACTGTT40370.15536489969400388No Hit
CACCTGCTGCTGCCCAGCTTGCAGATACCTTGTTTAGCAAGGGTCTGGGA40090.15428731307239574No Hit
GTCTCCTGTCCCCATAGACGCTGAAGCGGAGGGGCATTGGCCTCGATGGG40070.15421034259942373No Hit
CCCATCTGAGCCGCCATCTGGCCCATGGAGCTGCCCTGGAGGGGGAGATG39970.15382549023456368No Hit
TCCTAGGTCAGCGGTGTACCATTGACATTGACGAGTGTATCTCCAAGCCC39550.15220911030215145No Hit
ACCCCCATCTCTCCTCCTCCGCTTCTCCCTTGGCATCCCACAGCTCTAAA39490.1519781988832354No Hit
CCATCCCAGGGCCTGAACCTGGACGACTGCTCCATGTATGAGGACATCTC39260.15109303844405728No Hit
ACAAGTTCAGCAGCCCCAACAAGTACAGATGCAAGTTCAACCTCAACAGT39130.15059273036973922No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC38820.14939968803867304No Hit
CGAGAATGTAGTCGATGACGCGCTGTAGGATTTCCACCTGGCTAAGCTGA38720.149014835673813No Hit
CGCCTGTCTCCACCACCTGAGGAGTCACCCATGTCCCCTCCACCTGAAGA38700.14893786520084096No Hit
CCTGGCTCCTCAGGCCCTTCATCCGGCTCCAGCTCTGGCCCATCGAGGCC38690.14889937996435498No Hit
CAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAGGACGAGGTGA38550.1483605866535509No Hit
GAACACACTGGCGGTTGTCAATTTCCAGAAAGACTTTAGAGCTGTGGGAT38440.14793724905220484No Hit
GAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGGTAA38400.14778330810626084No Hit
ACTCCCGCCTGCGGGAACTGGTACCCGGAGTCCCGAGAGGCACTCAGCTT38390.1477448228697748No Hit
CCAGACCCACTTCGAGAGCAAGACAGAGCTGCTGCCTTCCCGGCCTCACG37780.14539722344412848No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT37770.14535873820764247No Hit
GACCTCCCCATGGGGACAGAAGGAGCTCTGAGTCCTGGAGTGAGCACATC37540.14447357776846434No Hit
GGCAGAGAACCTCACCTGGAAAATGACAAGGAGAGCCACCACCAGCACGC37120.14285719783605214No Hit
CCCCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGAT37100.1427802273630801No Hit
GCCACATGGGAAGGGGGTGAGGTGTTCCACGGGCACGATGCGCACGCCAC37070.1426647716536221No Hit
TCATCTGCAGACGCAGAGTCTGGAGCATGGGCAGGCGGGCCAGTGGCCGG36870.141895066923902No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT36760.14147172932255592No Hit
TACCTGCAGAGCAAGGAATGTGACTTCCAGACCAAATTTGCACTCAGCCT36290.13966292320771367No Hit
ATGCCTTAGACATGCTGGTGCTGTTCAGGGTATGGGGCCTGGGAGGTGAT36210.13935504131582563No Hit
CTGGCCTAGGGGAAGACACAGCCCCTCTGGATGGGATTGATGCTCCGGGT36040.13870079229556354No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA35840.13793108756584344No Hit
CCACCTCCTGCTCTGCCTGAGGAGGGGTGAGAGCCAGCGATGGTGTCCTA35670.13727683854558134No Hit
GCACCACTCTGCATTTGCCTAGCCTGCCTTCCTCTAACTGTGCTCTCCCT35630.1371228975996373No Hit
AGCCAAACTGCCAGGGGTCTGTCCAGGCTCTGGCTGTGAACCCGGAGCAT35340.13600682574154316No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT35230.1355834881401971No Hit
ACCACCTCATGTTCCTCCTAAGAGAAGTGGCCTTACCTTGTCTTTCACAT34800.13392862297129887No Hit
GCACGGCATCTTCTTCCGCTCACTCGATGAGACCACGCTCCGGCCACTGG34680.13346680013346682No Hit
CCCTTCCCTGGCCCTGGCAGAACCTGAATGGCGTGATGGTGGCAGTGGCG34620.13323588871455078No Hit
GACCCCATTGATCGACGGCCATGGACACAGCAGCACCTTGAAGCTGCAGA34620.13323588871455078No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC34610.13319740347806477No Hit
GGACCTGGGTCTTCAGTGAACCATTGTTCAATATCGTCCGGGGACAGCAT34480.1326970954037467No Hit
ACCCTCTGCTGTTGTCACATGCTTCCGAGGGGCTGGTGGGCAGGGTGCGT34470.1326586101672607No Hit
GACAAAGACACATCGTTGCTGAAAGAGATTAGGAACAGGTGAGGGGCAGC34360.1322352725659146No Hit
CAAGGACCCGGAGGCTACCCACCGGCCATGCAGCAGCAGCAGCGCATGCA34160.13146556783619454No Hit
GGCAGCAACATGCATGGCGACAGCAGTTTCTTGGGTTTTCCGGTTCATGC33890.13042646645107236No Hit
CAGGGAGAGGAGGCTACCCGTGAGCTGGAGACTAAGTGGCTGCCCCTCAC33620.12938736506595022No Hit
CTGGCCCAGGCTGGGGGGCATCTGCAGGTGAGGCCTGGAAGTAGGGAGAG33570.1291949388835202No Hit
AACCCCCAAGCTGTCTGTAGTCACACACAGGGAAGGGCAGGAGGCAGGGA33300.12815583749839804No Hit
GGCCTTCTTGCTGACAGGTATCATTTCTGTGAGAAGTGTTTCACAGAGAT33210.127809470370024No Hit
TGTTGCCATGACCTTGCTCTACTGAACCACAAGGAGGGCTAGAGATGTGGTCTGCATCCATGTGTCCTTGGATA32930.12673188374841585No Hit
GCTCTCCTTGGCTGCTGGAAATCCCTGATGTGCTCACTCCAGGACTCAGA32910.12665491327544384No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC32870.12650097232949983No Hit
TATCCAAGGACACATGGATGCAGACCACATCTCTAGCCCTCCTTGTGGTTCAGTAGAGCAAGGTCATGGCAACA32450.12488459239708759No Hit
CTCCAGGCAGTGGCAGTGCTGGAAGCTTGAGTAGGCTCCATCCAGTCCCA32400.12469216621465756No Hit
GGGGCTGTGAAGGGTCGTCACCCAGGGTCACATTCTCGCCCTGGATCTCT32350.12449974003222754No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT32070.12342215341061939No Hit
GGAAAGGGGCAGATTAGGCAACCGAATGAGGAAGATCTCCTTACCAATGT32040.12330669770116137No Hit
GAAGCCACACAGCCTGTGTTGAAGCAGAGGAGGCGGCTCACAATGAAAGA32020.12322972722818937No Hit
GCCTGTTCTCTGCTTCCCCTTACCTAGGTATGACAAATACTGTGCAGACC31880.12269093391738528No Hit
CCAGGACCCTGCTGCTGTTGCTGCTGGATTGCCACCTGTCCTAGAAGGTG31700.12199819966063719No Hit
GTCACGGCAGCATGAGCACGGCGGCGCCCAGGGCGGGGGCGGAAGGGCCT31650.12180577347820716No Hit
GATCCCCATCAAGTACAAGGCAATGAAGAAAGAGTTCCCCAGCATCCTGA31570.1214978915863191No Hit
TGGTCTGAGGGAGAGCTGTCTTGTCATGGATTATATCACCTCCCAGGCCC31550.12142092111334711No Hit
ACCAAACATCTCATTGTACTGGGTCTCATTCACCTCCATGCGGTTCATCC31520.12130546540388909No Hit
GGTGCAGGGGTTGGTGTAGTCGCAGACAGTGATGAACCTCAGGATGCTGG31220.12015090830930891No Hit
CCGCGTTGTCAAGGACAAGATCGGCGAGGAGCAGCGCAAGACCATCTGGG31210.12011242307282291No Hit
GGCAGTCGCGCAGGGGAAGTGGTAAAGCCGTGGACATCCAGGTGAGCGGC31190.1200354525998509No Hit
CCCAGCAGCGCTTCCCTCCACCGCAGGTAAGATATCCCTGCCTCCTGCCC31120.11976605594444886No Hit
CCCTCTCTTCTCTGCAGAAAAGAAGGACACAGTGTTGCGGCAGGTACGCC31060.11953514452553284No Hit
GGAGACATTGTAGGTATAGCTGTGCTGAGTGGGTGGCTCTGGGGCGGGGC31000.11930423310661681No Hit
CAAGGGCAAGTAGGGAGATTTGGCACCAGGTCCCCAATCTTCTTGTCAAT30760.11838058743095267No Hit
GCCAATGCCCAGGACAACATGGGCCGCTGTCCACTCCATGCTGCAGTGGC30640.1179187645931206No Hit
CAGAGTGAAACACCCACTTACTTGCAGCACTGCTTCTTTATATTGCGACC30570.11764936793771859No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA30530.11749542699177455No Hit
CAGTCTCTAAAACTGAAGAATGACATTGAAAATCGGCCCAAGAAGGAGCA30450.11718754509988652No Hit
AGAAAGTGGAGCATGTCATCAAAGATAGGCTGCAAGTCACAGGGGTCCAG30380.11691814844448448No Hit
GGCCCAGAAACTGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCACC30360.11684117797151246No Hit
CCTTGAAAGCCCTAGACTCTCAAATCCTCATGTGCCCTCAAACAAGCTAC29780.11460903425532414No Hit
AGGGGTGGGCACACCGGTCGGCTCTGGTCAGGCCTAGGAGATGCAGGTAG29670.11418569665397808No Hit
GCCACCTCAGGTTGGGTCAGGGATGTGTCCCCCACCAGGGGGCATGAACC29520.11360841810668801No Hit
CCAGGAGGGTACGCTTCTGGTACCCCCTTTACCTGCCAAGTTCCTCGCCG29130.11210749388373378No Hit
CTTCTCATGTTCTTTACCTGGAAGACACCTTGGGCATCAGCTGCCACTGC28890.11118384820806965No Hit
AGCACATTACTTGGAAACACAGACCTCAAATGACTTTTCCAGTGTGAAGT28660.11029868776889153No Hit
GCTGCTGGGCCGGAGCAGCCCCTCCAAGAACTACGTGATGGCAGTCTCCA28640.11022171729591954No Hit
CAAGACAGGCACGGCAATACTCCCCTCCATGTGGCATGTAAAGATGGCAA28520.10975989445808747No Hit
CCAAAGACTGGCTCGCAGGGCCTCACATGAAAGAGGTCCTACCTGCATCT28510.10972140922160145No Hit
GCACCCCTGGTCACAGTGGTTGTCTTTGAAGTGGTCTGCACAGTATTTGT28400.1092980716202554No Hit
GCCCGCCTCACAGCTGGTCCTCGTCCACCCAGATGGTCTTGCGCTGCTCC28270.10879776354593733No Hit
ACCAAGGAATCTGCTTTTCAAGTATGCCACCAACAGTGAAGAGGTAAGCC28150.10833594070810526No Hit
GAGGAGCTGGCACTAATGATACAGGGCACCCTCCTACAGGAGAGCCCCGC28100.10814351452567524No Hit
CTCCAGGTGGATGCATGCAGGCTGTGAGAGCCTCTTCACAGAGGACGATG27960.10760472121487115No Hit
GACCGTGTTCAGGCAGGAAAACGTGGATGATTACTACGACACCGGCGAGG27820.10706592790406709No Hit
TGCCACCCTCTGGATTGAGCATCCACCAAGAACTTTTTCCAGTTCCTTCA27780.10691198695812307No Hit
GGGAGTCCCAGGCTTCAGGAGACTCCTCAGGACTGCAGGTAGCTTGTTTG27760.10683501648515105No Hit
CTGTCCCTACACAGCAAGTTCATTCGTGTGCAGAATGAAGGAACTGGAAA27660.106450164120291No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG27600.10621925270137497No Hit
ACAGAGGCCCAGCTGTAGTTCTATTACCAAGTTTTGTACCTTTGTAGCCT27550.10602682651894495No Hit
CTCCGGATGTGACACCTTGCTTTTGTACCATTAACTTCACACTGGAAAAG27440.10560348891759887No Hit
GTTGTCGTCGTTGCAAATTCTGTCACGTTTGTGGAAGGCAACATCAGGCT27360.10529560702571084No Hit
CACACCCCAATGGCGGGATAGCAGCCCCTGTGTGTGAGCACCCCTCCATG27300.10506469560679481No Hit
TTCACCCTGGCAGCGCTGCTCCCACTGCACCAGGCTCGGTGCCTCCATCC27140.10444893182301872No Hit
GCATCTTCCGGCCACACTCCTTGCAATCAACGAAACTAGGAGGCAAAGAA26880.10344831567438258No Hit
GTGGGGACTGAGAGCCGCAGGACGTGCACTTCGGGGGGTGCATGTAGAGT26860.10337134520141056No Hit
AGAGAGGTATAGAGGGGATAACAGATGCCGAGTACCTGGCATGGAGGGGT26830.10325588949195255No Hit
GGCGAGGGGAAGGAGGGGGCTCCAGGGGGCGGCATGTAGTGGTGCACCAG26830.10325588949195255No Hit
CCCCGGAGCACGCAGCCCGTGCCCCGCTTCGACTGCGTGCTCAAGCTGGT26760.10298649283655051No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG26740.10290952236357849No Hit
ACCACGTAGGGCTGGCAGGAGACACAGTCAAAGCCTTCATCGGCTGCCTG26660.10260164047169046No Hit
CACAGCGGCTACCCGAGAATGGTGGGGCACACTGGCAGGAGTAATAAGGA26610.10240921428926043No Hit
ACACCAGTCACCTCCATACTCACTGCCCATGCCCCATCCTCTCCTTCATC26600.10237072905277442No Hit
AGTAACGTACTGTTTGCTGATGAAGCCAATGATCAGCCTGGCCGGGATGA26350.10140859814062428No Hit
ACCCCGAGTGCCTGGTGGGCAAGAGCACTTGAAATGGTTCACAAGGTCAA26200.1008313195933342No Hit
ATTCTGCTGGGGGTAATCATGGCAAAACGAAAGCGTAAGCATGGCTCTCT26180.10075434912036219No Hit
GCATACCTTGTTGGAGATGTCCAAATTGCAGTTTGCTTCACAGAGGGCCA26180.10075434912036219No Hit
ACAGCATTTGCCATCCACATACGCAAGAGCCGACTTAAGTGGGCAGTCAG26060.10029252628253012No Hit
AGGAGTTGGGGATCTTCATGCTCAGCAGCTCCGCCACTGCCACCATCACG26040.10021555580955813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)