FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004965400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803447
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN628973.48759902564367No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG53070.2942698066535917No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA40400.22401545484840973No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG38260.21214928966584545No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT37910.21020856171542604No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT36720.20361008668400016No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG30570.16950872412663084No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA29070.16119131862483344No Hit
ACCTATCGCTTTGAAAAAGAAAGGTATTGCTTGGTGGACAGATAAAAATG23930.1326903424386744No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC23920.1326348930686624No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG23340.1294188296079674No Hit
AGCACATTACTTGGAAACACAGACCTCAAATGACTTTTCCAGTGTGAAGT23180.1285316396877757No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC22850.12670181047738027No Hit
CTCCGGATGTGACACCTTGCTTTTGTACCATTAACTTCACACTGGAAAAG22200.1230976014266014No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG21970.12182226591632579No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG21880.12132322158621796No Hit
GAAGTGGACTTTGCTATTAACGTATGCACTCTCCTATCAAATGAAAGCAA21840.12110142410617002No Hit
GTCTCCTCCAGGGGGATTTCTGAATTTCACATTTTTATCTGTCCACCAAG21640.11999243670593038No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT21570.11960429111584649No Hit
CCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGG21520.11932704426578658No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT20900.11588918332504365No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG20870.1157228352150077No Hit
CAACATTTTAATTAAAATGATTTACCTTAACGAAGTCTCTATCAGTCTTCTCTTTCCATTCTTCTCCAAATAC20550.11394845537462425No Hit
TACAACATGTATTGTGCTGTATATGAATGGTATGTATGCTTTTTAAAACAAAATAGTTTGAAAACTTGC20410.1131721641944565No Hit
GTATTTGGAGAAGAATGGAAAGAGAAGACTGATAGAGACTTCGTTAAGGTAAATCATTTTAATTAAAATGTTG20300.11256222112432471No Hit
CAACAGATAAATTTTAGTTAATAAAACATGTTTTCTTATTTTAAAACAAGGACAATTTCAAAGGATCAGTTTG20090.11139778435407308No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC19960.11067694254391729No Hit
ACAGCATTTGCCATCCACATACGCAAGAGCCGACTTAAGTGGGCAGTCAG19750.10951250577366566No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC19720.1093461576636297No Hit
CAAACTGATCCTTTGAAATTGTCCTTGTTTTAAAATAAGAAAACATGTTTTATTAACTAAAATTTATCTGTTG19510.10818172089337807No Hit
GCAAGTTTTCAAACTATTTTGTTTTAAAAAGCATACATACCATTCATATACAGCACAATACATGTTGTA19390.10751632845323429No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG19360.10734998034319834No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT19290.10696183475311445No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG19240.10668458790305455No Hit
TAGGATCTTTTTCAAGTTGCATGACGTGCTTGCTTTCATTTGATAGGAGA19220.10657368916303057No Hit
CAAGGGATATTCACCATAAAGGTATCTGATCTTTGATATACTTAAGGTAT19050.10563104987282687No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA19030.10552015113280291No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG18810.10430026499253929No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC18700.10369032192240747No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT18690.10363487255239551No Hit
GGACAGCCCATAACTTTGACGCAGATAATCTAACAAAGAAAAAAAATACC18370.10186049271201204No Hit
CAAGTTTTTCTAAAGACCTTGGATTAACAGGTTGATTACTAATGCTGATG18220.10102875216183231No Hit
CTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGGGTAAGTTCTTGCTTTGTTCAA18180.1008069546817844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)