FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004965401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803447
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN630203.4944192981551443No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG53570.29704227515419085No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA40730.22584528405880516No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG37070.20555081463441952No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT37070.20555081463441952No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT36920.20471907408423978No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA28590.15852974886425827No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG27660.1533729574531439No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG23500.13030601952815912No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC23410.1298069751980513No Hit
ACCTATCGCTTTGAAAAAGAAAGGTATTGCTTGGTGGACAGATAAAAATG23340.1294188296079674No Hit
AGCACATTACTTGGAAACACAGACCTCAAATGACTTTTCCAGTGTGAAGT23040.12775534850760795No Hit
GTCTCCTCCAGGGGGATTTCTGAATTTCACATTTTTATCTGTCCACCAAG22390.12415113945682907No Hit
CTCCGGATGTGACACCTTGCTTTTGTACCATTAACTTCACACTGGAAAAG22080.1224322089864576No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC21780.12076872788609812No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG21490.11916069615575063No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG21460.11899434804571468No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT20960.11622187954511555No Hit
GAAGTGGACTTTGCTATTAACGTATGCACTCTCCTATCAAATGAAAGCAA20750.11505744277486392No Hit
CCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGG20730.11494654403483996No Hit
GTATTTGGAGAAGAATGGAAAGAGAAGACTGATAGAGACTTCGTTAAGGTAAATCATTTTAATTAAAATGTTG20500.11367120852456435No Hit
GCAAGTTTTCAAACTATTTTGTTTTAAAAAGCATACATACCATTCATATACAGCACAATACATGTTGTA20330.11272856923436064No Hit
CAACATTTTAATTAAAATGATTTACCTTAACGAAGTCTCTATCAGTCTTCTCTTTCCATTCTTCTCCAAATAC20300.11256222112432471No Hit
ACAGCATTTGCCATCCACATACGCAAGAGCCGACTTAAGTGGGCAGTCAG20280.11245132238430072No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC20270.11239587301428876No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT20120.11156413246410901No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT20070.11128688561404909No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC20040.11112053750401314No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG19980.11078784128394124No Hit
CAAACTGATCCTTTGAAATTGTCCTTGTTTTAAAATAAGAAAACATGTTTTATTAACTAAAATTTATCTGTTG19920.11045514506386936No Hit
CAACAGATAAATTTTAGTTAATAAAACATGTTTTCTTATTTTAAAACAAGGACAATTTCAAAGGATCAGTTTG19700.10923525892360574No Hit
TAGGATCTTTTTCAAGTTGCATGACGTGCTTGCTTTCATTTGATAGGAGA19530.10829261963340203No Hit
TACAACATGTATTGTGCTGTATATGAATGGTATGTATGCTTTTTAAAACAAAATAGTTTGAAAACTTGC19410.10762722719325825No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG19210.1065182397930186No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT19040.10557560050281488No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG18900.10479930932264714No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA18880.10468841058262315No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC18860.1045775118425992No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG18820.10435571436255126No Hit
CAAGGGATATTCACCATAAAGGTATCTGATCTTTGATATACTTAAGGTAT18800.1042448156225273No Hit
GGACAGCCCATAACTTTGACGCAGATAATCTAACAAAGAAAAAAAATACC18340.1016941446019761No Hit
CAAGTTTTTCTAAAGACCTTGGATTAACAGGTTGATTACTAATGCTGATG18170.1007515053117724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)