FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114924
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN452462.1393676557644623No Hit
GCTGCTGCAGCTTGATGACCTCGTTCATCAGGAAGTGCGTGAGGCCCTCG127240.6016291838382845No Hit
CCCAGTCCTCAGGCCTGACTGGGTGCCCCCTCTCCACAGTGGAGGAAGGC125370.5927872585492434No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG110660.5232339318103156No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG78430.37084074888743046No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT75730.3580743326946973No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG69690.3295153868413238No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA63220.2989232710017003No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG51220.2421836434784418No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA50330.23797545443713344No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT45390.21461764110672538No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG39070.18473477061114252No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC35440.16757103328535683No Hit
CACGGAGCGAGAGAGCACAGCGAGGGCCACAGAGGGTGCAGAGCGGGAGA35330.1670509200330603No Hit
CAAGACCCTGGAGGGCGAGTTCTCGGTCACCATGTGGTCCTCAGGTGAGC34330.16232261773945542No Hit
CAAAACAAAAACATCCCGGGGCCGGGGCGCGCGGCCGGCGCTCACCTGAG34140.16142424030367047No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG33630.159012806133932No Hit
CAGCCTCCTCCTGCTGCTGCTTCAAGATATACTTTTGGCAATCCTCCTCT32960.15584484359721673No Hit
GGCCTGGAGGCTATCCAGCGTGAGTCTCTCCTACCCTCCCGCTCTGGTCC32590.15409537174858293No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG31760.15017088084489089No Hit
CCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAA31380.14837412597332103No Hit
ACTTGAAAAGCAGATTCCTTGGTAAGGATGCCTCCGCCTGAACCGAAGTA31020.14667193714762328No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT30230.1429365783356754No Hit
GAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGCCAAGCGCAAAGCGTCTG29950.14161265369346607No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG29770.14076155928061718No Hit
GAGAGCGGCCAAAGATACGCCGCTGCGCTCCTTGGAGGCGGCAACAGCTT29720.14052514416593695No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG29620.14005231393657644No Hit
GTGGCCCACGCCCTGCATGCCAAAGGGCTGGCTGTTGTAGCTGAGGTCCA29320.138633823248495No Hit
CACACCCCAATGGCGGGATAGCAGCCCCTGTGTGTGAGCACCCCTCCATG29300.13853925720262286No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT29200.1380664269732624No Hit
GAGCTGTGCGTAGCGCATGGCTAAGTTGTAGTTGTCGTCCTTCACCTTGA29030.13726261558334957No Hit
CCCCGCGCGGTGAAGGGCGTCAGGTGCAGCTGGCTGGACATCTCGGCGAA29030.13726261558334957No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG28750.1359386909411402No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG28550.13499303048241923No Hit
GTGGACAGAGGCTGAGTTTGAAGAGAAGTGTACATACATTGTGAACGACC28500.13475661536773897No Hit
CCACCTGACCCTCCGCCAGGCCGGCGACGACTTCTCCCGCCGCTACCGCC28310.13385823793195403No Hit
AGAGAGGTATAGAGGGGATAACAGATGCCGAGTACCTGGCATGGAGGGGT28090.132818011427361No Hit
CAAACTAACCAACCCACTGGGAGAAGATGCCTGGGGGTCCAGGAGTCCTC27940.13210876608332026No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC27850.13168321887689582No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA27790.13139952073927952No Hit
ACCCAGGTAGACAGCAGACAGCAGGAAGAGGAGGAAGATGGTGGCAGGCA27740.1311631056245993No Hit
GTGGTCGTGGTCTTCATAGTCTTCCAGCTGCCCTACAATGGGGTGGTCCT27530.13017016214294228No Hit
AGCCCCATTAAGACCAATAACCCCATGGCCAGGGGTTTCCATCAGTTACC27130.12827884122550032No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC27120.12823155820256427No Hit
ACGCCTTCATCGGCGTCAAGTTCCGCAACGATCTCTTCAAGCTCTTCAAG26830.12686035053741884No Hit
GAAGGAAGAGCGGGACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACG26760.12652936937686649No Hit
CTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTA26740.1264348033309944No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC26460.12511087868878504No Hit
AAAGAGGTAGGCACTCCCAGGGAGGCTGCTTTACTCTGTCTCTTCCCCAC26430.12496902961997688No Hit
GGAACAGACAAACTATCGACTGAAGTTGTGTGTGTCTGACCGCGATGTCC26410.12487446357410478No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA26270.12421250125300011No Hit
GTGTGGAGATATTCCTTTCGAGCATGACGAAGAGATCATCAGGGGCCAGG26160.12369238800070358No Hit
GCCGACAGGAAGACCACTGCCGGAGCTGCTCCTGGCTGAGGCAGCCCAGG26070.12326684079427912No Hit
AGGCGATGTTGAGTTGCTTACTGAGCTCACAGGTGCTACTGGTGATGTTG26010.12298314265666284No Hit
TCCAGTGTGATGATGGTGAGGATGGGCCTCCGGTTCATGCCGCCCATGCA26000.12293585963372679No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT25880.12236846335849422No Hit
GGTCAGGTTACGCAGCAAGGCCATGCCAGTTTCTGTCAGCCACACACCTT25800.12199019917500582No Hit
CTCTGAAATCACCTGTTAACGATGTTATTGAGCCGGCTGGCTTGTGGGAT25700.12151736894564533No Hit
GGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCT25660.12132823685390114No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG25590.12099725569334879No Hit
GACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCAT25420.12019344430343597No Hit
CTGCTCGCTCACGCAGCCCCGGGTCCTCAGGAGGCTGGAGAGGAAGCCCA25160.1189640857070987No Hit
CAGAACCAATGGAAGTGGATGAAAAGAAACCTGAAGTGAAAGTAGAAGTT24910.11778201013369749No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA24880.11764016106488934No Hit
AGGACATCTCTGATTGGAGGGGGGACTCTTGCTCATAGGAGATGATGGTC24730.11693091572084859No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG24630.11645808549148812No Hit
AAGGAAATACTACCTTTTCTTAAACGGAAGATGAATGTCAGTGCTACACC24560.11612710433093576No Hit
GAGCCCGAGCCGCGCGCGACATGAGCCACGGGAAGGGAACCGACATGCTC24530.11598525526212763No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA24410.11541785898689504No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC24280.1148031796887264No Hit
ATGTCGAAAAGTGTTTCTGTCATCCAAATACTCCACACGCAAATTTCCTT24250.11466133061991826No Hit
CAGGAGAGCCCCTACCTTTTTTGGCTCCCTCAGGAACAATCCTGTACACT24190.11437763248230196No Hit
TCGATTTTTATTTTTATTTTTAAAGAGGGAGACGATGGACTGAGCTGATC24160.1142357834134938No Hit
CCGAAGCTCCGCTGATGCGGTTGTCCGACAGGTCCACGTAGCGCAGGCCA24140.11414121736762171No Hit
GAACAAGAGCAATGACTTTGAGGAACTGGTTGAGCGGAGCCAGCTGGACA24080.11385751923000544No Hit
GCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAATT24040.11366838713826125No Hit
ACAAAAGCAAGGTGTCACATCCGGAGATAAACATTTATTACAGGGACTGG24040.11366838713826125No Hit
CAGTAAGACCCGAGACTCCATGGTGCGGATCAGCTCAGTCCATCGTCTCC24010.11352653806945309No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA23930.1131482738859647No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT23870.1128645757483484No Hit
GCGTCTGCAGATGAACTTCATCAACCAGGCCCAGCTCGGCATCTTCAGGG23860.11281729272541235No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA23830.1126754436566042No Hit
GAGAGGCTGTGCCGTTACTGCTACTCTCTTCTTCCTCTTTAACTTCTACT23800.11253359458779606No Hit
TGTCATCCACGTCCTGGAGCCAGGAGATGAACTGGCGGGCGTTGAAACGG23790.11248631156486001No Hit
TCGATGAGCTCACTAGCAATAGACCAGACACAGGTGCCAGGCAGGACATC23640.11177706622081929No Hit
CCCTGACTCTGTCCCACTGCCCAGGTACATTAACCATTCCTGTGCCCCTA23550.11135151901439484No Hit
CTTTCAGCCAATAGCAATCCTTGTGAGCATGCAGGAAAATGTGTGAACAC23510.11116238692265065No Hit
GCCCTCGAGGCCACGGGATTCTTCATCTGCAATATCTGGACCATCATCTC23410.11068955669329016No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG23400.11064227367035412No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT23270.11002759437218547No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA23160.10950748111988895No Hit
AGCAGCACCAGCAATCTCAAGACCCACCTGCGACTCCATTCTGGAGAGAA23130.1093656320510808No Hit
CCAGGTGGCTCCGTGAAAGAGACCTCCAGGAAGGCAGTCAGCCCCGGCTC23100.10922378298227264No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT23100.10922378298227264No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC23030.1088928018217203No Hit
GTACCAAGCAATGTGCCTTTAAAAGAGGTCCTATGGAAAAAACAAAAGGA22950.10851453763823192No Hit
ACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGT22930.10841997159235983No Hit
AGCTGACGTGAAGATGAGCAGCTCAGAGGAGGTGTCCTGGATTTCCTGGT22890.10823083950061561No Hit
TTTGTCCTCTTTGTCAAATGTCACGACTTCGGCCACACAGTTAGGGGCAC22820.10789985834006327No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC22790.10775800927125515No Hit
ACAGAGAAGACTCCAACGATAAAATCAGCGAGGTCTCCTCTGACAGCTTC22760.10761616020244699No Hit
CTGTAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAG22720.1074270281107028No Hit
CCTCCTTAGCCCACTGATGAACGATGGCTCCAACTCTGGTAACATTGGAA22630.10700148090427836No Hit
GCAGGGCCGGGCGCAGAGTGCCATGGGCGGCCTCTCTTATACACAGCAGG22620.10695419788134232No Hit
CCATAACAGCTTTTTTCTCTCCTTGAAGTATTTGTCTGAAAGTGGCCACC22580.1067650657895981No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG22560.10667049974372601No Hit
GCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCG22530.10652865067491787No Hit
CCACAGGGTGACGAGAACAAGTCAGTGCGCGACCGTTTCAACGCCCGCCA22510.10643408462904576No Hit
CAGCTTTCTATCACATAGTGAAGGACGACGCATTGTCGTTATCTGCGGTC22490.10633951858317367No Hit
GAACCAATCAAGCATTGCCCAAGATCGAGGTGAGAGCCTGGGTGTTGGGG22420.10600853742262133No Hit
GACCCAGTCCCGGGTGCTCAGTTCCCCCCAGCTGGCACAGCAGGGTCAGG22360.10572483928500505No Hit
CTCACAACGAGGTCCTGCATAACCCTTCAGACACTCACAGTGGAAGGCGC22330.1055829902161969No Hit
ATATTTTTCTCTTGCAGATTGATTATACCGGAACAGAGCCTTCCAGTCCC22310.1054884241703248No Hit
GACTGCTTCCTACATCGTAAGTGCAATTATTGTACGTTTTCATTTTCTAT22300.10544114114738874No Hit
GCAGCCGCTGGTCACCGACGACCCGACCCAGGTGCACCACCACCAGGAGG22270.1052992920785806No Hit
CTGTAGGAAGAGACACCTCTGTTTCTTGCTGCCCGGGAGGGGAGCTATGA22260.10525200905564455No Hit
CTGCCGAAGCCTTGGCGTTCTCAGGTGAGTGCAGGGTTTGCTCCTGGAGG22160.10477917882628406No Hit
CAAGCAGTGCTGCCAGCTCCTTGAAAAAGCAGTATATCCAGTGTCTCTAT22110.10454276371160381No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC22020.10411721650517938No Hit
CGGCGCGCAGCCCGTCCGAGTCGTCGCCGCCCACCACCTCCTCGCGCGTC21980.10392808441343519No Hit
GCACGCACACACACAAAACAACCCTGAGGGTGCCTCACCAGGCTCCAGGT21960.10383351836756309No Hit
GGACAGCAACAAGGGTCAAGGTAATCACAATCACCATCTACCTGCTGTGT21930.10369166929875494No Hit
CACCCAGCCCAAACTACCAAAAATATACATATGGCCAATCCAGCATGAGC21920.10364438627581889No Hit
CCCGCAGGCGGGAGTAGCAGTGGTTCATGTCGTCCAGCAAGCTCAGCGGC21890.10350253720701075No Hit
ACATCCAGGACAAATTTAATCTTACTGGACTCAATGAGCAGGTCCCTCAC21880.1034552541840747No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA21850.10331340511526654No Hit
GCATTTTAAGACTCTTACCATGTCCTTCATCACACAATCTACATCTTCTG21810.10312427302352235No Hit
AGGGCACTCAAATGTCTGCCCTAGCTCCCTGCCCCTCCCCAACACCCAGG21780.1029824239547142No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC21780.1029824239547142No Hit
CATGCGGTGCTTGTACATTTCACAGTCCTTTCCCAGGGTCGAGCACTTGA21720.10269872581709792No Hit
GGGTGAACTTGGCAGGGCACACCTTGCATTGGTATGGTTTCTCTCCAGAA21640.10232046163360953No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC21620.10222589558773743No Hit
CCATTTAGTTGACTATCATCTCGAGATTTCACGTAACGACGATGGTTTTGATAGAAATTAGACAGTCCAT21600.10213132954186534No Hit
GTACTTGATGGGGATCAGTCGCTTCTGATGGGCACCTGCAAGCCAAGGGG21570.10198948047305718No Hit
GATACTAGAGCCGCTGCCTCCTCGTAGAAGCTCCGACAGTTGTTTATGTT21500.10165849931250484No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG21450.1014220841978246No Hit
AACCCTGGGGTTGAAGACTGGGCTTGTCCCACCATGGGGCAAGGGCCTGA21420.10128023512901646No Hit
GTTTTCTCTGCAGTCTTTAAACCAGAGGAGTTACGCCAGGCCCTCATGCC21410.10123295210608041No Hit
AAAATCCTCACACACTACAAACGGTGAGCTTGTGATGCTGGAGCCAAGGG21380.10109110303727226No Hit
TTTAAAAGATGAGAAAGCTCTGAATTCAGAATTTTCCAGTTCTGCAACTT21340.10090197094552807No Hit
AGGAGGGGTAGTACTGGGAGGTCCCCTTCATGCCCGAAGGGGACAGGGGC21340.10090197094552807No Hit
CCCCACCAGGCTGGCTTCCTGTCAGGCGAGCTGGCCCTCAACAGCCCCGG21310.10076012187671991No Hit
AACACGGCCTTCAAACAGCAACGGCTGCCAGCTTGGCAGCCCATCCTTAC21270.10057098978497572No Hit
CTGATCTGTATCCTAAATCCTCATAATGGGACCAGAGGATCCCTGTCAAC21180.10014544257855129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)