FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004965403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114924
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN445842.108066294580798No Hit
CCCAGTCCTCAGGCCTGACTGGGTGCCCCCTCTCCACAGTGGAGGAAGGC125600.5938747680767724No Hit
GCTGCTGCAGCTTGATGACCTCGTTCATCAGGAAGTGCGTGAGGCCCTCG121910.5764273326133705No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG107320.5074414021496754No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG77340.3656868993874012No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT75610.35750693641946474No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG69200.3271985187174575No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA59530.2814758355382983No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG50710.2397722093087033No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA49710.23504390701509842No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT40910.1934348468313755No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG36250.17140095814317677No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG35130.1661052595743393No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC33910.16033673077614136No Hit
CAAAACAAAAACATCCCGGGGCCGGGGCGCGCGGCCGGCGCTCACCTGAG33500.15839812683576335No Hit
CAAGACCCTGGAGGGCGAGTTCTCGGTCACCATGTGGTCCTCAGGTGAGC33210.15702691917061795No Hit
GGCCTGGAGGCTATCCAGCGTGAGTCTCTCCTACCCTCCCGCTCTGGTCC33170.15683778707887375No Hit
CACGGAGCGAGAGAGCACAGCGAGGGCCACAGAGGGTGCAGAGCGGGAGA33060.1563176738265772No Hit
CAGCCTCCTCCTGCTGCTGCTTCAAGATATACTTTTGGCAATCCTCCTCT31790.15031272991369904No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG31580.149319786432042No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG31100.14705020133111166No Hit
CCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAA30310.14331484251916382No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT29630.1400995969595125No Hit
GTGGACAGAGGCTGAGTTTGAAGAGAAGTGTACATACATTGTGAACGACC29250.13830284208794263No Hit
GAGAGCGGCCAAAGATACGCCGCTGCGCTCCTTGGAGGCGGCAACAGCTT28910.13669521930811698No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT28800.13617510605582045No Hit
ACTTGAAAAGCAGATTCCTTGGTAAGGATGCCTCCGCCTGAACCGAAGTA28700.13570227582645997No Hit
AGAGAGGTATAGAGGGGATAACAGATGCCGAGTACCTGGCATGGAGGGGT28700.13570227582645997No Hit
GTGGCCCACGCCCTGCATGCCAAAGGGCTGGCTGTTGTAGCTGAGGTCCA28480.13466204932186687No Hit
CCACCTGACCCTCCGCCAGGCCGGCGACGACTTCTCCCGCCGCTACCGCC28310.13385823793195403No Hit
GAAGAAGAAGGCCCGCAAGTCTGCAGGTGCGGCCAAGCGCAAAGCGTCTG28270.13366910584020986No Hit
GAAGGAAGAGCGGGACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACG28230.13347997374846568No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG28110.1329125774732331No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC28100.13286529445029702No Hit
CACACCCCAATGGCGGGATAGCAGCCCCTGTGTGTGAGCACCCCTCCATG28090.132818011427361No Hit
CAAACTAACCAACCCACTGGGAGAAGATGCCTGGGGGTCCAGGAGTCCTC27850.13168321887689582No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG27740.1311631056245993No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC27070.127995143087884No Hit
ACCCAGGTAGACAGCAGACAGCAGGAAGAGGAGGAAGATGGTGGCAGGCA26990.1276168789043956No Hit
CCCCGCGCGGTGAAGGGCGTCAGGTGCAGCTGGCTGGACATCTCGGCGAA26920.12728589774384327No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT26680.1261511051933781No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC26600.12577284100988972No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG26530.12544185984933737No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG26360.12463804845942454No Hit
TCCAGTGTGATGATGGTGAGGATGGGCCTCCGGTTCATGCCGCCCATGCA26330.12449619939061639No Hit
GAGCTGTGCGTAGCGCATGGCTAAGTTGTAGTTGTCGTCCTTCACCTTGA26290.1243070672988722No Hit
AGCCCCATTAAGACCAATAACCCCATGGCCAGGGGTTTCCATCAGTTACC26180.12378695404657568No Hit
GTGGTCGTGGTCTTCATAGTCTTCCAGCTGCCCTACAATGGGGTGGTCCT26120.12350325590895937No Hit
GCCGACAGGAAGACCACTGCCGGAGCTGCTCCTGGCTGAGGCAGCCCAGG26100.12340868986308727No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA26090.12336140684015123No Hit
AAAGAGGTAGGCACTCCCAGGGAGGCTGCTTTACTCTGTCTCTTCCCCAC26070.12326684079427912No Hit
AGGCGATGTTGAGTTGCTTACTGAGCTCACAGGTGCTACTGGTGATGTTG26010.12298314265666284No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA25990.12288857661079075No Hit
ACGCCTTCATCGGCGTCAAGTTCCGCAACGATCTCTTCAAGCTCTTCAAG25980.12284129358785469No Hit
GACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCAT25890.12241574638143025No Hit
CTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTA25820.12208476522087791No Hit
AAGGAAATACTACCTTTTCTTAAACGGAAGATGAATGTCAGTGCTACACC25570.1209026896474767No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA25080.1185858215236103No Hit
GTGTGGAGATATTCCTTTCGAGCATGACGAAGAGATCATCAGGGGCCAGG25060.1184912554777382No Hit
GGAACAGACAAACTATCGACTGAAGTTGTGTGTGTCTGACCGCGATGTCC24690.11674178362910441No Hit
GCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAATT24510.11589068921625553No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG24420.11546514200983107No Hit
GAGAGGCTGTGCCGTTACTGCTACTCTCTTCTTCCTCTTTAACTTCTACT24280.1148031796887264No Hit
TCGATGAGCTCACTAGCAATAGACCAGACACAGGTGCCAGGCAGGACATC24230.11456676457404615No Hit
AGGACATCTCTGATTGGAGGGGGGACTCTTGCTCATAGGAGATGATGGTC24150.11418850039055778No Hit
TCGATTTTTATTTTTATTTTTAAAGAGGGAGACGATGGACTGAGCTGATC24070.11381023620706937No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT24070.11381023620706937No Hit
CTCTGAAATCACCTGTTAACGATGTTATTGAGCCGGCTGGCTTGTGGGAT24040.11366838713826125No Hit
GGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCT24000.11347925504651703No Hit
GAACAAGAGCAATGACTTTGAGGAACTGGTTGAGCGGAGCCAGCTGGACA23990.11343197202358099No Hit
GAGCCCGAGCCGCGCGCGACATGAGCCACGGGAAGGGAACCGACATGCTC23950.11324283993183679No Hit
TGTCATCCACGTCCTGGAGCCAGGAGATGAACTGGCGGGCGTTGAAACGG23930.1131482738859647No Hit
GGTCAGGTTACGCAGCAAGGCCATGCCAGTTTCTGTCAGCCACACACCTT23930.1131482738859647No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC23890.11295914179422051No Hit
GCGTCTGCAGATGAACTTCATCAACCAGGCCCAGCTCGGCATCTTCAGGG23860.11281729272541235No Hit
CAGAACCAATGGAAGTGGATGAAAAGAAACCTGAAGTGAAAGTAGAAGTT23820.11262816063366816No Hit
CTGCTCGCTCACGCAGCCCCGGGTCCTCAGGAGGCTGGAGAGGAAGCCCA23610.11163521715201113No Hit
ACAAAAGCAAGGTGTCACATCCGGAGATAAACATTTATTACAGGGACTGG23570.11144608506026693No Hit
CAGGAGAGCCCCTACCTTTTTTGGCTCCCTCAGGAACAATCCTGTACACT23460.1109259718079704No Hit
CCGAAGCTCCGCTGATGCGGTTGTCCGACAGGTCCACGTAGCGCAGGCCA23400.11064227367035412No Hit
CAGTAAGACCCGAGACTCCATGGTGCGGATCAGCTCAGTCCATCGTCTCC23290.11012216041805757No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA23240.10988574530337732No Hit
GCCCTCGAGGCCACGGGATTCTTCATCTGCAATATCTGGACCATCATCTC23230.10983846228044128No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA23170.10955476414282499No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA23100.10922378298227264No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC23080.10912921693640054No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT23020.10884551879878426No Hit
CCATAACAGCTTTTTTCTCTCCTTGAAGTATTTGTCTGAAAGTGGCCACC22990.10870366972997611No Hit
CCCTGACTCTGTCCCACTGCCCAGGTACATTAACCATTCCTGTGCCCCTA22980.10865638670704006No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC22860.10808899043180749No Hit
ACAGAGAAGACTCCAACGATAAAATCAGCGAGGTCTCCTCTGACAGCTTC22750.10756887717951094No Hit
CTTTCAGCCAATAGCAATCCTTGTGAGCATGCAGGAAAATGTGTGAACAC22730.10747431113363884No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA22730.10747431113363884No Hit
ATGTCGAAAAGTGTTTCTGTCATCCAAATACTCCACACGCAAATTTCCTT22600.10685963183547022No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA22560.10667049974372601No Hit
GGACAGCAACAAGGGTCAAGGTAATCACAATCACCATCTACCTGCTGTGT22510.10643408462904576No Hit
GCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCG22450.10615038649142948No Hit
GCATTTTAAGACTCTTACCATGTCCTTCATCACACAATCTACATCTTCTG22320.10553570719326083No Hit
TTTAAAAGATGAGAAAGCTCTGAATTCAGAATTTTCCAGTTCTGCAACTT22260.10525200905564455No Hit
GTTTTCTCTGCAGTCTTTAAACCAGAGGAGTTACGCCAGGCCCTCATGCC22250.1052047260327085No Hit
CCAGGTGGCTCCGTGAAAGAGACCTCCAGGAAGGCAGTCAGCCCCGGCTC22220.10506287696390036No Hit
TTTGTCCTCTTTGTCAAATGTCACGACTTCGGCCACACAGTTAGGGGCAC22220.10506287696390036No Hit
CTCACAACGAGGTCCTGCATAACCCTTCAGACACTCACAGTGGAAGGCGC22150.10473189580334802No Hit
AGCAGCACCAGCAATCTCAAGACCCACCTGCGACTCCATTCTGGAGAGAA22090.10444819766573171No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG22060.10430634859692359No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC21940.103738952321691No Hit
GGGTGAACTTGGCAGGGCACACCTTGCATTGGTATGGTTTCTCTCCAGAA21890.10350253720701075No Hit
ACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGT21890.10350253720701075No Hit
ATATTTTTCTCTTGCAGATTGATTATACCGGAACAGAGCCTTCCAGTCCC21700.10260415977122583No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG21660.10241502767948162No Hit
CCACAGGGTGACGAGAACAAGTCAGTGCGCGACCGTTTCAACGCCCGCCA21630.10227317861067349No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT21600.10213132954186534No Hit
CTGTAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAG21600.10213132954186534No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA21580.10203676349599324No Hit
CGGCGCGCAGCCCGTCCGAGTCGTCGCCGCCCACCACCTCCTCGCGCGTC21570.10198948047305718No Hit
CAAGCAGTGCTGCCAGCTCCTTGAAAAAGCAGTATATCCAGTGTCTCTAT21530.101800348381313No Hit
GCAGGGCCGGGCGCAGAGTGCCATGGGCGGCCTCTCTTATACACAGCAGG21480.10156393326663275No Hit
ACATCCAGGACAAATTTAATCTTACTGGACTCAATGAGCAGGTCCCTCAC21380.10109110303727226No Hit
GTACCAAGCAATGTGCCTTTAAAAGAGGTCCTATGGAAAAAACAAAAGGA21380.10109110303727226No Hit
AGGGCACTCAAATGTCTGCCCTAGCTCCCTGCCCCTCCCCAACACCCAGG21360.10099653699140015No Hit
TCCCAGCCCACGGAACCAACTGGCATTCAGCCAGGAGAGAGAACTGTCCT21310.10076012187671991No Hit
GCACGCACACACACAAAACAACCCTGAGGGTGCCTCACCAGGCTCCAGGT21300.10071283885378388No Hit
AACCAGGGCATCTGCCCCTGGTCCTGTCTCCACAGAGCACTACAAACACC21290.10066555583084782No Hit
GAACCAATCAAGCATTGCCCAAGATCGAGGTGAGAGCCTGGGTGTTGGGG21280.10061827280791179No Hit
CACCCAGCCCAAACTACCAAAAATATACATATGGCCAATCCAGCATGAGC21250.10047642373910363No Hit
AAACAGTGGGAACCTTGTTCCAGATGCTGCTTCCAAACATAAACAACTGT21180.10014544257855129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)