FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125609
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN102280.4811797466043849No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG50030.23536784046360362No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG43120.20285950990986584No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG33820.15910734288385117No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG32930.15492030754480246No Hit
CGCAAAAAGAAAATATATGAAGAGAAAAACTTACTTGCAGTATCCTGTGC32340.15214463243239937No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA31490.1481457784569034No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT30070.14146534005078074No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT29530.1389248916428186No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT29150.13713716868906747No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC26260.12354106517238118No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA23410.11013314301924766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)