FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125609
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN100920.47478158024359135No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG47540.22365355058244482No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG44170.20779927070312554No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG34400.16183597265536606No Hit
CGCAAAAAGAAAATATATGAAGAGAAAAACTTACTTGCAGTATCCTGTGC33350.15689621186210634No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA32660.1536500833408214No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG32480.152803267204834No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT31580.1485691865248971No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT30380.14292374561831456No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT28530.13422035755399983No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC26650.12537583346702053No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA22760.10707519586151545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)