FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3370156
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3127489.27992650785305No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC415051.231545364665612No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG405821.204157908417296No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG263490.7818332445144972No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG261930.7772043786697115No Hit
CAACAGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC151840.4505429422258198No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCTGTTG148250.43989061633942167No Hit
CGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC116610.34600772189774004No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCG107310.31841256013074765No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAACAACAACAGC101170.3001938189211419No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA99640.29565396972721736No Hit
CAGGTGGTGGTGACAGGCGTGATGCCTCAGGTGGTGGGG96500.28633689360373826No Hit
GCTGTTGTTGTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG96440.2861588603020157No Hit
GGGCAAGCTGGAGTACCTGTACCTGCAGGCCAACCTCATCGAGGTGGTC94990.28185638884372116No Hit
CCCCAGGCACTGATACTCACCTGACTGAGGTTGAAGGTCATGATGCTGTT92720.27512079559521874No Hit
GACCACCTCGATGAGGTTGGCCTGCAGGTACAGGTACTCCAGCTTGCCC92040.27310308484236334No Hit
CCCCACCACCTGAGGCATCACGCCTGTCACCACCACCTG86370.25627893782958416No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC82950.24613103963139984No Hit
TGTTGCCATGACCTTGCTCTACTGAACCACAAGGAGGGCTAGAGATGTGGTCTGCATCCATGTGTCCTTGGATA79890.23705134124355076No Hit
TATCCAAGGACACATGGATGCAGACCACATCTCTAGCCCTCCTTGTGGTTCAGTAGAGCAAGGTCATGGCAACA79000.23441051393466653No Hit
CAAACAGTTGTACAATCTCCTGGGAGGTCCATGACCTCTGCTTGCTCAGT76570.22720016521490402No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA74500.22105801630547664No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG72040.21375865093485286No Hit
GACCTCCCCATGGGGACAGAAGGAGCTCTGAGTCCTGGAGTGAGCACATC72020.21369930650094537No Hit
AAAGGCCTCCACATGCTCTGTGATGAGAGAGCATCGCATCGCTTGCTCAC69500.20622190782859903No Hit
CCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAA65270.19367056005716057No Hit
GCTCTCCTTGGCTGCTGGAAATCCCTGATGTGCTCACTCCAGGACTCAGA64690.19194957147384276No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG63610.1887449720428372No Hit
GCACCACTCTGCATTTGCCTAGCCTGCCTTCCTCTAACTGTGCTCTCCCT53900.15993324938074083No Hit
ACACACAGGCATGGGCTCCTACCAGCAGAACTCCATGGGGAGCTATGGTC51890.15396913377303603No Hit
AACCCCCAAGCTGTCTGTAGTCACACACAGGGAAGGGCAGGAGGCAGGGA51560.1529899506135621No Hit
CCCTCCTCCCGGCCAGAGGCACGGCTGCAGCACCGCAGCCCACGCCTACC51460.15269322844402453No Hit
CCAGGACCCTGCTGCTGTTGCTGCTGGATTGCCACCTGTCCTAGAAGGTG50650.15028977887077039No Hit
GTAGCTGCCCTGGGTGTTATGGCAGAGACCATGGTTCATGCAGGGCTTGG50370.14945895679606522No Hit
TCCTAGGTCAGCGGTGTACCATTGACATTGACGAGTGTATCTCCAAGCCC50330.1493402679282502No Hit
ATGCCTTAGACATGCTGGTGCTGTTCAGGGTATGGGGCCTGGGAGGTGAT50210.14898420132480514No Hit
GGCCCAGAAACTGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCACC49760.14764895156188615No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA49350.1464323906667822No Hit
TGGTCTGAGGGAGAGCTGTCTTGTCATGGATTATATCACCTCCCAGGCCC48020.14248598581193275No Hit
GACCTGTCCCAGAAGCTGTATGCCACCATGGAGAAGCACAAGGAGGTAGG47850.1419815581237189No Hit
GACAAAGACACATCGTTGCTGAAAGAGATTAGGAACAGGTGAGGGTCAGC47530.14103204718119874No Hit
CAGGGAGAGGAGGCTACCCGTGAGCTGGAGACTAAGTGGCTGACCCTCAC47060.13963745298437225No Hit
CCGCGTTGTCAAGGACAAGATCGGCGAGGAGCAGCGCAAGACCATCTGGG47010.13948909189960346No Hit
ACTGACCTTGTGGGCCATACTGACCCCCCTGGGGACCATAGCTCCCCATG46730.13865826982489832No Hit
GCCGAGTCCCCACTGGCCACCATGTGGGGCACGCAGGAGC46410.13770875888237816No Hit
TCATCTGCAGACGCAGAGTCTGGAGCATGGGCAGGCGGGCCAGTGGCCGG46030.13658121463813544No Hit
GCCTGTTCTCTGCTTCCCCTTACCTAGGTATGACAAATACTGTGCAGACC45790.13586908143124532No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG45220.13417776506488127No Hit
GGCAGCAACATGCATGGCGACAGCAGTTTCTTGGGTTTTCCGGTTCATGC44940.13334694299017613No Hit
GCACGGCATCTTCTTCCGCTCACTCGATGAGACCACGCTCCGGCCACTGG44270.1313589044542745No Hit
GCCCGCCTCACAGCTGGTCCTCGTCCACCCAGATGGTCTTGCGCTGCTCC43940.1303797212948006No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC43310.128510371626714No Hit
GTCTCCTGTCCCCATAGACGCTGAAGCGGAGGGGCATTGGCCTCGATGGG43290.12845102719280652No Hit
CCTGGCTCCTCAGGCCCTTCATCCGGCTCCAGCTCTGGCCCATCGAGGCC43270.128391682758899No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT43240.12830266610803773No Hit
ACCCCGAGTGCCTGGTGGGCAAGAGCACTTGAAATGGTTCACAAGGTCAA42880.12723446629770255No Hit
CCCAGCAGCGCTTCCCTCCACCGCAGGTAAGATATCCCTGCCTCCTGCCC42790.12696741634511874No Hit
GCCACATGGGAAGGGGGTGAGGTGTTCCACGGGCACGATGCGCACGCCAC42380.12575085545001477No Hit
CTGGCCCAGGCTGGGGGGCATCTGCAGGTGAGGCCTGGAAGTAGGGAGAG41980.12456396677186456No Hit
CAGCCCTGCCAGAATGGAGGCACCTGTATTGACCTTGTGAACCATTTCAA41890.12429691681928078No Hit
GCACCCCTGGTCACAGTGGTTGTCTTTGAAGTGGTCTGCACAGTATTTGT40620.12052854526615384No Hit
ACCCCCATCTCTCCTCCTCCGCTTCTCCCTTGGCATCCCACAGCTCTAAA39850.1182437845607147No Hit
GAACACACTGGCGGTTGTCAATTTCCAGAAAGACTTTAGAGCTGTGGGAT39780.11803607904203842No Hit
GCTCCTGCGTGCCCCACATGGTGGCCAGTGGGGACTCGGC39730.11788771795726964No Hit
CAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAGGACGAGGTGA39320.11667115706216566No Hit
GCTCGTGGTAGAGGTCCAGCTTATTGTGGGACAGGTCTAGCACCTGCAGA39240.11643377932653563No Hit
CGCAGGCAGTCAATGGCTCCCAGTTCCTGCCGCTGACCGGTCTGCAGGTG39190.11628541824176684No Hit
GCCAGAGCAGGGTTGAGCTTACCTGGAGAGAGGCTGAGTGCAAATTTGGT38590.11450508522454154No Hit
CCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGG38300.11364459093288262No Hit
ACAGCATTTGCCATCCACATACGCAAGAGCCGACTTAAGTGGGCAGTCAG38190.11331819654639133No Hit
GGAGACATTGTAGGTATAGCTGTGCTGAGTGGGTGGCTCTGGGGCGGGGC38010.11278409664122373No Hit
GCCACCTCAGGTTGGGTCAGGGATGTGTCCCCCACCAGGGGGCATGAACC37900.11245770225473241No Hit
CAGAGTGAAACACCCACTTACTTGCAGCACTGCTTCTTTATATTGCGACC37800.11216098008519487No Hit
GAGGACACTACACTTACTTATTGCAGTCAATGTGTAAAATCAAATGGGAA37790.11213130786824112No Hit
CCCGAAAAGCCGTCCAAGGGCTCCGGCTACCGCTGCATTCGCATCAACCA37710.11189393013261108No Hit
GAGCACACAGAGCACCTGTCATTTTCCAACACCCAAATCTGACCATTGTG37360.11085540253922964No Hit
GAGGAGCTGGCACTAATGATACAGGGCACCCTCCTACAGGAGAGCCCCGC37350.11082573032227588No Hit
GCCAATGCCCAGGACAACATGGGCCGCTGTCCACTCCATGCTGCAGTGGC37350.11082573032227588No Hit
CAGAGGATCTTCCCACCTCAGCCTCCTGAGAAGTGGGACTACAGGTGC36440.10812555857948417No Hit
TACCTGCAGAGCAAGGAATGTGACTTCCAGACCAAATTTGCACTCAGCCT36400.10800686971166915No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT36260.10759145867431656No Hit
CTTCTCATGTTCTTTACCTGGAAGACACCTTGGGCATCAGCTGCCACTGC36250.1075617864573628No Hit
GAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGGTAA36160.107294736504779No Hit
GCACAGGGGACTGCATCCATGATGGAAAAGTTAAGCACAATGGTCAGATT35890.10649358664702761No Hit
ACAGCAGAGGAACCTCCAGTCTCAGCACCATGAATCAAACTGCCATTCTG35860.10640456999616635No Hit
GCATCTTCCGGCCACACTCCTTGCAATCAACGAAACTAGGAGGCAAAGAA35840.10634522556225882No Hit
CCCTTCCCTGGCCCTGGCAGAACCTGAATGGCGTGATGGTGGCAGTGGCG35670.10584079787404499No Hit
GCAGCCCCAGCATCAGGCCCTTCCCACAGGGGAGCCCTGGCTCC35370.10495063136543234No Hit
GGTTCCGTGTGCTTTAACAGTGCGCCTTCTTTGCCTCCTAGTTTCGTTGA35360.10492095914847858No Hit
GCCCACAGCTGGTACAGAAGAACAGGTCACACAACTCCCCTGGCCCCTCA35310.1047725980637098No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCC35250.10459456476198728No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG35230.10453522032807976No Hit
CTCCGGATGTGACACCTTGCTTTTGTACCATTAACTTCACACTGGAAAAG35220.10450554811112603No Hit
AGCACATTACTTGGAAACACAGACCTCAAATGACTTTTCCAGTGTGAAGT35140.10426817037549596No Hit
GCACCTGTAGTCCCACTTCTCAGGAGGCTGAGGTGGGAAGATCCTCTG35110.10417915372463471No Hit
GCCACTTAGAGTCAGAAAGATAAGGCAGCAAATCAGAATGGCAGTTTGAT35080.10409013707377344No Hit
GGCGCAATCATGACACAGTGAGAAATCATGAGACCACTGTGCATCCCACC35020.1039121037720509No Hit
ACTAATTGCTTAGATAAGCCCAAGTTTGGTGGTCGCAATATAAAGAAGCA35020.1039121037720509No Hit
GGAGCCAGGGCTCCCCTGTGGGAAGGGCCTGATGCTGGGGCTGC35020.1039121037720509No Hit
GACGCCGATGAAGGCGTACAAGAAAGGGTTGACGCAGCAGCGGACGCAGG35000.1038527593381434No Hit
ACAAGTTCAGCAGCCCCAACAAGTACAGATGCAAGTTCAACCTCAACAGT34670.10287357617866948No Hit
GGCTCTCCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG34450.10222078740568685No Hit
GTTCGCTGTAAGAGCTGTGGATCCACAACTCCAGGCAAAGGGTGGGATGC34100.10118225981230543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)