FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3370156
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3138349.312150535464827No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG407671.2096472685537405No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC401211.1904790164016146No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG264510.7848598106437803No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG248640.7377700023381707No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCTGTTG148880.4417599660075083No Hit
CAACAGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC147330.43716077237967615No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCG113190.33585982369955575No Hit
CGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC107910.320192893147973No Hit
GCTGTTGTTGTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG99590.29550560864244857No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA97580.2895414930347438No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAACAACAACAGC96220.28550607152903307No Hit
CCCCACCACCTGAGGCATCACGCCTGTCACCACCACCTG95200.28247950539975003No Hit
GACCACCTCGATGAGGTTGGCCTGCAGGTACAGGTACTCCAGCTTGCCC94900.2815893388911374No Hit
GGGCAAGCTGGAGTACCTGTACCTGCAGGCCAACCTCATCGAGGTGGTC91030.2701061909300341No Hit
CCCCAGGCACTGATACTCACCTGACTGAGGTTGAAGGTCATGATGCTGTT88560.26277715334245655No Hit
TATCCAAGGACACATGGATGCAGACCACATCTCTAGCCCTCCTTGTGGTTCAGTAGAGCAAGGTCATGGCAACA81360.24141315713575276No Hit
CAGGTGGTGGTGACAGGCGTGATGCCTCAGGTGGTGGGG80720.2395141352507124No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC78360.23251149204962618No Hit
TGTTGCCATGACCTTGCTCTACTGAACCACAAGGAGGGCTAGAGATGTGGTCTGCATCCATGTGTCCTTGGATA77430.22975197587292695No Hit
CAAACAGTTGTACAATCTCCTGGGAGGTCCATGACCTCTGCTTGCTCAGT76290.22636934314019885No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA74870.22215588833276562No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG73840.2190996499865288No Hit
GACCTCCCCATGGGGACAGAAGGAGCTCTGAGTCCTGGAGTGAGCACATC72890.21628078937592207No Hit
CCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAA68510.20328435835017727No Hit
AAAGGCCTCCACATGCTCTGTGATGAGAGAGCATCGCATCGCTTGCTCAC64310.19082202722960007No Hit
GCTCTCCTTGGCTGCTGGAAATCCCTGATGTGCTCACTCCAGGACTCAGA62600.18574807813050792No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG62170.18447217280149641No Hit
ACACACAGGCATGGGCTCCTACCAGCAGAACTCCATGGGGAGCTATGGTC55060.16337522654737643No Hit
GCACCACTCTGCATTTGCCTAGCCTGCCTTCCTCTAACTGTGCTCTCCCT52340.155304383535955No Hit
GTAGCTGCCCTGGGTGTTATGGCAGAGACCATGGTTCATGCAGGGCTTGG52070.15450323367820362No Hit
CCCTCCTCCCGGCCAGAGGCACGGCTGCAGCACCGCAGCCCACGCCTACC51720.15346470608482218No Hit
AACCCCCAAGCTGTCTGTAGTCACACACAGGGAAGGGCAGGAGGCAGGGA50830.15082387877593798No Hit
CCAGGACCCTGCTGCTGTTGCTGCTGGATTGCCACCTGTCCTAGAAGGTG50620.1502007622199091No Hit
ATGCCTTAGACATGCTGGTGCTGTTCAGGGTATGGGGCCTGGGAGGTGAT50560.15002272891818658No Hit
GGCCCAGAAACTGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCACC48310.14334648010359163No Hit
GACAAAGACACATCGTTGCTGAAAGAGATTAGGAACAGGTGAGGGTCAGC47820.14189254147285763No Hit
TCCTAGGTCAGCGGTGTACCATTGACATTGACGAGTGTATCTCCAAGCCC47380.1405869639268924No Hit
GACCTGTCCCAGAAGCTGTATGCCACCATGGAGAAGCACAAGGAGGTAGG46740.13868794204185206No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA46620.138331875438407No Hit
TGGTCTGAGGGAGAGCTGTCTTGTCATGGATTATATCACCTCCCAGGCCC45810.13592842586515283No Hit
CAGGGAGAGGAGGCTACCCGTGAGCTGGAGACTAAGTGGCTGACCCTCAC45710.13563170369561528No Hit
GCCCGCCTCACAGCTGGTCCTCGTCCACCCAGATGGTCTTGCGCTGCTCC45550.1351569482243552No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC44390.1317149710577196No Hit
GCCTGTTCTCTGCTTCCCCTTACCTAGGTATGACAAATACTGTGCAGACC44390.1317149710577196No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG44230.13124021558645949No Hit
CCGCGTTGTCAAGGACAAGATCGGCGAGGAGCAGCGCAAGACCATCTGGG43920.13032037686089307No Hit
GCACGGCATCTTCTTCCGCTCACTCGATGAGACCACGCTCCGGCCACTGG43710.12969726030486423No Hit
TCATCTGCAGACGCAGAGTCTGGAGCATGGGCAGGCGGGCCAGTGGCCGG43480.12901479931492785No Hit
ACCCCGAGTGCCTGGTGGGCAAGAGCACTTGAAATGGTTCACAAGGTCAA42480.12604757761955232No Hit
CAGCCCTGCCAGAATGGAGGCACCTGTATTGACCTTGTGAACCATTTCAA42130.12500905002617088No Hit
GCTCCTGCGTGCCCCACATGGTGGCCAGTGGGGACTCGGC42130.12500905002617088No Hit
GTCTCCTGTCCCCATAGACGCTGAAGCGGAGGGGCATTGGCCTCGATGGG41920.12438593347014204No Hit
CCTGGCTCCTCAGGCCCTTCATCCGGCTCCAGCTCTGGCCCATCGAGGCC41780.12397052243278946No Hit
ACTGACCTTGTGGGCCATACTGACCCCCCTGGGGACCATAGCTCCCCATG41510.12316937257503807No Hit
GCACCCCTGGTCACAGTGGTTGTCTTTGAAGTGGTCTGCACAGTATTTGT41500.1231397003580843No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT41480.1230803559241768No Hit
CTGGCCCAGGCTGGGGGGCATCTGCAGGTGAGGCCTGGAAGTAGGGAGAG41370.12275396153768549No Hit
CCCAGCAGCGCTTCCCTCCACCGCAGGTAAGATATCCCTGCCTCCTGCCC40610.12049887304920011No Hit
GCCGAGTCCCCACTGGCCACCATGTGGGGCACGCAGGAGC39880.11833280121157595No Hit
GCCACATGGGAAGGGGGTGAGGTGTTCCACGGGCACGATGCGCACGCCAC39870.11830312899462221No Hit
ACCCCCATCTCTCCTCCTCCGCTTCTCCCTTGGCATCCCACAGCTCTAAA39610.11753165135382455No Hit
CAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAGGACGAGGTGA39600.11750197913687083No Hit
GCTCGTGGTAGAGGTCCAGCTTATTGTGGGACAGGTCTAGCACCTGCAGA39530.11729427361819453No Hit
GCCACCTCAGGTTGGGTCAGGGATGTGTCCCCCACCAGGGGGCATGAACC39280.11655246819435065No Hit
GAGGACACTACACTTACTTATTGCAGTCAATGTGTAAAATCAAATGGGAA39000.11572164611964549No Hit
GCCAGAGCAGGGTTGAGCTTACCTGGAGAGAGGCTGAGTGCAAATTTGGT38920.11548426838401546No Hit
GGCAGCAACATGCATGGCGACAGCAGTTTCTTGGGTTTTCCGGTTCATGC38560.11441606857368027No Hit
CCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGG38390.11391164088546642No Hit
GAACACACTGGCGGTTGTCAATTTCCAGAAAGACTTTAGAGCTGTGGGAT37800.11216098008519487No Hit
CCCGAAAAGCCGTCCAAGGGCTCCGGCTACCGCTGCATTCGCATCAACCA37550.11141917466135098No Hit
CGCAGGCAGTCAATGGCTCCCAGTTCCTGCCGCTGACCGGTCTGCAGGTG36780.10913441395591184No Hit
GCACAGGGGACTGCATCCATGATGGAAAAGTTAAGCACAATGGTCAGATT36420.10806621414557664No Hit
ACAGCATTTGCCATCCACATACGCAAGAGCCGACTTAAGTGGGCAGTCAG36380.10794752527776162No Hit
GCACCTGTAGTCCCACTTCTCAGGAGGCTGAGGTGGGAAGATCCTCTG36360.10788818084385413No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT36290.10768047532517783No Hit
GAGGAGCTGGCACTAATGATACAGGGCACCCTCCTACAGGAGAGCCCCGC36060.10699801433524145No Hit
GGAGACATTGTAGGTATAGCTGTGCTGAGTGGGTGGCTCTGGGGCGGGGC36060.10699801433524145No Hit
ACTAATTGCTTAGATAAGCCCAAGTTTGGTGGTCGCAATATAAAGAAGCA35910.10655293108093514No Hit
TACCTGCAGAGCAAGGAATGTGACTTCCAGACCAAATTTGCACTCAGCCT35870.10643424221312012No Hit
CAGAGTGAAACACCCACTTACTTGCAGCACTGCTTCTTTATATTGCGACC35760.1061078478266288No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCC35720.10598915895881378No Hit
GGAGCCAGGGCTCCCCTGTGGGAAGGGCCTGATGCTGGGGCTGC35660.10581112565709123No Hit
GACGCCGATGAAGGCGTACAAGAAAGGGTTGACGCAGCAGCGGACGCAGG35560.1055144034875537No Hit
AGCACATTACTTGGAAACACAGACCTCAAATGACTTTTCCAGTGTGAAGT35420.10509899245020111No Hit
ACAGCAGAGGAACCTCCAGTCTCAGCACCATGAATCAAACTGCCATTCTG35140.10426817037549596No Hit
CTTCTCATGTTCTTTACCTGGAAGACACCTTGGGCATCAGCTGCCACTGC35140.10426817037549596No Hit
GCCCACAGCTGGTACAGAAGAACAGGTCACACAACTCCCCTGGCCCCTCA35110.10417915372463471No Hit
GAGCACACAGAGCACCTGTCATTTTCCAACACCCAAATCTGACCATTGTG35030.10394177598900467No Hit
GCCAATGCCCAGGACAACATGGGCCGCTGTCCACTCCATGCTGCAGTGGC34980.10379341490423588No Hit
CAGAGGATCTTCCCACCTCAGCCTCCTGAGAAGTGGGACTACAGGTGC34940.10367472603642086No Hit
GCATCTTCCGGCCACACTCCTTGCAATCAACGAAACTAGGAGGCAAAGAA34640.10278455952780821No Hit
GGTTCCGTGTGCTTTAACAGTGCGCCTTCTTTGCCTCCTAGTTTCGTTGA34390.10204275410396432No Hit
GGCGCAATCATGACACAGTGAGAAATCATGAGACCACTGTGCATCCCACC34390.10204275410396432No Hit
GGCTCTCCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG34230.10156799863270424No Hit
GAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGGTAA34050.10103389872753664No Hit
GCCACTTAGAGTCAGAAAGATAAGGCAGCAAATCAGAATGGCAGTTTGAT33820.10035143773760027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)