FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004965412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497271
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43719217.50679041241419No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC571242.2874569880481532No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG535712.14518168032224No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG238860.956484098041422No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG236130.9455521647430335No Hit
CAACAGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC117950.4723155796867861No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCTGTTG114130.4570188818113853No Hit
CGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC90920.364077426919225No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCG84830.33969080648435834No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAACAACAACAGC81450.32615603192444875No Hit
GCTGTTGTTGTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG77250.309337673003851No Hit
GGGCAAGCTGGAGTACCTGTACCTGCAGGCCAACCTCATCGAGGTGGTC73150.29291975120041036No Hit
GACCACCTCGATGAGGTTGGCCTGCAGGTACAGGTACTCCAGCTTGCCC70260.2813471185145705No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA67780.2714162780090747No Hit
CCGCGTTGTCAAGGACAAGATCGGCGAGGAGCAGCGCAAGACCATCTGGG66450.2660904643508854No Hit
CCCCAGGCACTGATACTCACCTGACTGAGGTTGAAGGTCATGATGCTGTT65610.2627267925667659No Hit
CAGGTGGTGGTGACAGGCGTGATGCCTCAGGTGGTGGGG64790.25944320820607775No Hit
CCCCACCACCTGAGGCATCACGCCTGTCACCACCACCTG62570.2505535042051904No Hit
GCCCGCCTCACAGCTGGTCCTCGTCCACCCAGATGGTCTTGCGCTGCTCC60920.24394629177209842No Hit
CCCTCCTCCCGGCCAGAGGCACGGCTGCAGCACCGCAGCCCACGCCTACC60000.2402622702942532No Hit
GACCTGTCCCAGAAGCTGTATGCCACCATGGAGAAGCACAAGGAGGTAGG59800.23946139605993902No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG54440.21799796658031906No Hit
GACCTCCCCATGGGGACAGAAGGAGCTCTGAGTCCTGGAGTGAGCACATC52310.20946865598487308No Hit
AAAGGCCTCCACATGCTCTGTGATGAGAGAGCATCGCATCGCTTGCTCAC49870.19969799032624014No Hit
CCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAA48270.1932909964517267No Hit
CCAGGACCCTGCTGCTGTTGCTGCTGGATTGCCACCTGTCCTAGAAGGTG47560.19044789291991138No Hit
GCTCTCCTTGGCTGCTGGAAATCCCTGATGTGCTCACTCCAGGACTCAGA46720.18708422113579182No Hit
GGCCCAGAAACTGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCACC46310.18544242895544777No Hit
GAGGAGCTGGCACTAATGATACAGGGCACCCTCCTACAGGAGAGCCCCGC43280.17330918430558798No Hit
GGAGACATTGTAGGTATAGCTGTGCTGAGTGGGTGGCTCTGGGGCGGGGC42990.17214791666583243No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC41630.16670197187249602No Hit
CCCCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGAT41450.16598118506161327No Hit
GCACCACTCTGCATTTGCCTAGCCTGCCTTCCTCTAACTGTGCTCTCCCT41280.16530044196244623No Hit
GGACCTGGGTCTTCAGTGAACCATTGTTCAATATCGTCCGGGGACAGCAT40340.16153633306116957No Hit
TCATCTGCAGACGCAGAGTCTGGAGCATGGGCAGGCGGGCCAGTGGCCGG40130.16069541511513968No Hit
ATGCCTTAGACATGCTGGTGCTGTTCAGGGTATGGGGCCTGGGAGGTGAT39790.1593339289168056No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG39570.15845296725906No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCATAGAGTATCTCGTATGC39190.15693130621386306No Hit
GTCTCCTGTCCCCATAGACGCTGAAGCGGAGGGGCATTGGCCTCGATGGG38660.15480898949293048No Hit
TGGTCTGAGGGAGAGCTGTCTTGTCATGGATTATATCACCTCCCAGGCCC38390.15372780927660634No Hit
CTGGCCCAGGCTGGGGGGCATCTGCAGGTGAGGCCTGGAAGTAGGGAGAG38060.15240636678998795No Hit
GCACGGCATCTTCTTCCGCTCACTCGATGAGACCACGCTCCGGCCACTGG37840.15152540513224236No Hit
CCTGGCTCCTCAGGCCCTTCATCCGGCTCCAGCTCTGGCCCATCGAGGCC37270.14924291356444697No Hit
AACCCCCAAGCTGTCTGTAGTCACACACAGGGAAGGGCAGGAGGCAGGGA36330.1454788046631703No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG36280.14527858610459177No Hit
TATCCAAGGACACATGGATGCAGACCACATCTCTAGCCCTCCTTGTGGTTCAGTAGAGCAAGGTCATGGCAACA35430.14187487060875653No Hit
TGTTGCCATGACCTTGCTCTACTGAACCACAAGGAGGGCTAGAGATGTGGTCTGCATCCATGTGTCCTTGGATA35330.14147443349159944No Hit
CAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAGGACGAGGTGA35000.14015299100498105No Hit
GCTCGTGGTAGAGGTCCAGCTTATTGTGGGACAGGTCTAGCACCTGCAGA34480.13807071799576418No Hit
CCCTTCCCTGGCCCTGGCAGAACCTGAATGGCGTGATGGTGGCAGTGGCG34120.13662914437399865No Hit
CAAACAGTTGTACAATCTCCTGGGAGGTCCATGACCTCTGCTTGCTCAGT34080.1364689695271358No Hit
GCCGAGTCCCCACTGGCCACCATGTGGGGCACGCAGGAGC33710.1349873521936546No Hit
GCCACATGGGAAGGGGGTGAGGTGTTCCACGGGCACGATGCGCACGCCAC33330.13346569114845766No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA33130.13266481691414347No Hit
CGCAGGCAGTCAATGGCTCCCAGTTCCTGCCGCTGACCGGTCTGCAGGTG33080.13246459835556496No Hit
CCCAGCAGCGCTTCCCTCCACCGCAGGTAAGATATCCCTGCCTCCTGCCC32700.130942937310368No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA32540.13030223792291665No Hit
GGCAGCAACATGCATGGCGACAGCAGTTTCTTGGGTTTTCCGGTTCATGC32330.12946131997688676No Hit
CCCATCTGAGCCGCCATCTGGCCCATGGAGCTGCCCTGGAGGGGGAGATG32090.12850027089570976No Hit
ACACACAGGCATGGGCTCCTACCAGCAGAACTCCATGGGGAGCTATGGTC31280.12525673024673734No Hit
CTGGCCTAGGGGAAGACACAGCCCCTCTGGATGGGATTGATGCTCCGGGT30900.12373506920154041No Hit
GCTCCTGCGTGCCCCACATGGTGGCCAGTGGGGACTCGGC30430.12185301475090209No Hit
GCCAGAGCAGGGTTGAGCTTACCTGGAGAGAGGCTGAGTGCAAATTTGGT30280.12125235907516645No Hit
CACCTGCTGCTGCCCAGCTTGCAGATACCTTGTTTAGCAAGGGTCTGGGA30200.12093200938144079No Hit
GCTCTGGCTTCTGGGTTTCTGCTAGGTTGTCTGGGGGATCCCAAGGTCCC29780.119250173489381No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT29230.11704776934501701No Hit
GCCACCTCAGGTTGGGTCAGGGATGTGTCCCCCACCAGGGGGCATGAACC29200.1169276382098699No Hit
TACCTGCAGAGCAAGGAATGTGACTTCCAGACCAAATTTGCACTCAGCCT29150.11672741965129134No Hit
CAAGGACCCGGAGGCTACCCACCGGCCATGCAGCAGCAGCAGCGCATGCA28930.11584645799354576No Hit
ACCCCCATCTCTCCTCCTCCGCTTCTCCCTTGGCATCCCACAGCTCTAAA28720.11500554004751587No Hit
AGCCAAACTGCCAGGGGTCTGTCCAGGCTCTGGCTGTGAACCCGGAGCAT28630.11464514664207448No Hit
GGAGCCAGGGCTCCCCTGTGGGAAGGGCCTGATGCTGGGGCTGC28470.11400444725462315No Hit
GAACACACTGGCGGTTGTCAATTTCCAGAAAGACTTTAGAGCTGTGGGAT28390.11368409756089748No Hit
CCCGAAAAGCCGTCCAAGGGCTCCGGCTACCGCTGCATTCGCATCAACCA27750.1111213000110921No Hit
AGGGGTGGGCACACCGGTCGGCTCTGGTCAGGCCTAGGAGATGCAGGTAG27630.1106407754705036No Hit
AGGAGTTGGGGATCTTCATGCTCAGCAGCTCCGCCACTGCCACCATCACG27500.11012020721819937No Hit
CCAAAGACTGGCTCGCAGGGCCTCACATGAAAGAGGTCCTACCTGCATCT27170.108798764731581No Hit
GCAGCCCCAGCATCAGGCCCTTCCCACAGGGGAGCCCTGGCTCC26610.10655631687550131No Hit
ACTGACCTTGTGGGCCATACTGACCCCCCTGGGGACCATAGCTCCCCATG26590.10647622945206987No Hit
GACAAAGACACATCGTTGCTGAAAGAGATTAGGAACAGGTGAGGGTCAGC26280.1052348743888829No Hit
AGCTGTCCCAGCATCGCACAATAGTGAGTCATCAGTCTCTGGCAGTGAGT25580.10243181456878328No Hit
GTTGAGGCTGCCCACATCCTGGTAGGTGCCCTGGAGGCCCCGGGAGATGT25280.10123050321731202No Hit
CCAAAGGGAAGGATGGGCTACATCACCCCCTTGCTTCTGCAGTCCAAACA25230.10103028465873348No Hit
CCAGCCCGAGATGAGGGCTCCCTACGGCTCTGTACTGACTCACTGCCAGA25120.10058980382986067No Hit
CAGGGAGAGGAGGCTACCCGTGAGCTGGAGACTAAGTGGCTGACCCTCAC25030.10022941042441931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)