FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497271
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43643117.476317147798536No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG542272.171450355207745No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC524652.1008933351646655No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG233170.9336992260751837No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG219890.8805211769167223No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCTGTTG112020.4485696586393707No Hit
CAACAGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC111830.44780882811677225No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCG86710.3472190242869116No Hit
CGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC84320.3376485771868572No Hit
GCTGTTGTTGTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG77220.30921754186870387No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAACAACAACAGC76700.307135268859487No Hit
GACCACCTCGATGAGGTTGGCCTGCAGGTACAGGTACTCCAGCTTGCCC70610.28274864842462033No Hit
AGATACCATCAACATCCTGCTGTATGATGACAACAGCATCATGACCTTCA67530.270415185216182No Hit
GGGCAAGCTGGAGTACCTGTACCTGCAGGCCAACCTCATCGAGGTGGTC67450.27009483552245633No Hit
GCCCGCCTCACAGCTGGTCCTCGTCCACCCAGATGGTCTTGCGCTGCTCC62860.25171477184494595No Hit
CCCCACCACCTGAGGCATCACGCCTGTCACCACCACCTG62520.2503532856466118No Hit
CCCTCCTCCCGGCCAGAGGCACGGCTGCAGCACCGCAGCCCACGCCTACC60830.24358589836665703No Hit
CCGCGTTGTCAAGGACAAGATCGGCGAGGAGCAGCGCAAGACCATCTGGG60680.24298524269092142No Hit
CCCCAGGCACTGATACTCACCTGACTGAGGTTGAAGGTCATGATGCTGTT60520.24234454330347008No Hit
CAGGTGGTGGTGACAGGCGTGATGCCTCAGGTGGTGGGG56570.22652727717576504No Hit
GACCTGTCCCAGAAGCTGTATGCCACCATGGAGAAGCACAAGGAGGTAGG56180.22496557241885243No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG53660.21487455706649378No Hit
GACCTCCCCATGGGGACAGAAGGAGCTCTGAGTCCTGGAGTGAGCACATC49670.19889711609192595No Hit
CCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAA47730.19112863601907842No Hit
AAAGGCCTCCACATGCTCTGTGATGAGAGAGCATCGCATCGCTTGCTCAC47690.19096846117221558No Hit
GCTCTCCTTGGCTGCTGGAAATCCCTGATGTGCTCACTCCAGGACTCAGA46270.18528225410858493No Hit
GGCCCAGAAACTGCAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCACC45900.1838006367751037No Hit
CCAGGACCCTGCTGCTGTTGCTGCTGGATTGCCACCTGTCCTAGAAGGTG44680.17891530394578722No Hit
GCACCACTCTGCATTTGCCTAGCCTGCCTTCCTCTAACTGTGCTCTCCCT41800.16738271497166307No Hit
GGAGACATTGTAGGTATAGCTGTGCTGAGTGGGTGGCTCTGGGGCGGGGC41460.16602122877332895No Hit
GAGGAGCTGGCACTAATGATACAGGGCACCCTCCTACAGGAGAGCCCCGC40280.16129607079087532No Hit
CCCCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGAT39940.15993458459254123No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC38770.15524947032180328No Hit
ATGCCTTAGACATGCTGGTGCTGTTCAGGGTATGGGGCCTGGGAGGTGAT38670.15484903320464619No Hit
GCACGGCATCTTCTTCCGCTCACTCGATGAGACCACGCTCCGGCCACTGG38450.1539680715469006No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG38150.15276676019542934No Hit
GGACCTGGGTCTTCAGTGAACCATTGTTCAATATCGTCCGGGGACAGCAT38090.15252649792513506No Hit
CCTGGCTCCTCAGGCCCTTCATCCGGCTCCAGCTCTGGCCCATCGAGGCC38000.1521661045196937No Hit
TCTCTTATACACATCTGACGCTGCCGACGACGCTATGTGTGTAGATCTCG37890.1517256236908209No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG37650.1507645746096439No Hit
TGGTCTGAGGGAGAGCTGTCTTGTCATGGATTATATCACCTCCCAGGCCC36780.14728077169037723No Hit
GTCTCCTGTCCCCATAGACGCTGAAGCGGAGGGGCATTGGCCTCGATGGG35600.14255561370792358No Hit
TCATCTGCAGACGCAGAGTCTGGAGCATGGGCAGGCGGGCCAGTGGCCGG35220.14103395266272664No Hit
TATCCAAGGACACATGGATGCAGACCACATCTCTAGCCCTCCTTGTGGTTCAGTAGAGCAAGGTCATGGCAACA34870.13963242275267682No Hit
AACCCCCAAGCTGTCTGTAGTCACACACAGGGAAGGGCAGGAGGCAGGGA34750.13915189821208832No Hit
CTGGCCCAGGCTGGGGGGCATCTGCAGGTGAGGCCTGGAAGTAGGGAGAG34260.13718975633801858No Hit
GCTCGTGGTAGAGGTCCAGCTTATTGTGGGACAGGTCTAGCACCTGCAGA33830.1354678767342431No Hit
TGTTGCCATGACCTTGCTCTACTGAACCACAAGGAGGGCTAGAGATGTGGTCTGCATCCATGTGTCCTTGGATA33800.13534774559909596No Hit
CCCTTCCCTGGCCCTGGCAGAACCTGAATGGCGTGATGGTGGCAGTGGCG33680.13486722105850749No Hit
CAAACAGTTGTACAATCTCCTGGGAGGTCCATGACCTCTGCTTGCTCAGT33630.1346670024999289No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA33170.13282499176100632No Hit
CAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAGGACGAGGTGA32680.13086284988693658No Hit
GCCACATGGGAAGGGGGTGAGGTGTTCCACGGGCACGATGCGCACGCCAC32210.12898079543629826No Hit
ACACACAGGCATGGGCTCCTACCAGCAGAACTCCATGGGGAGCTATGGTC31780.1272589158325228No Hit
CCCAGCAGCGCTTCCCTCCACCGCAGGTAAGATATCCCTGCCTCCTGCCC31750.12713878469737563No Hit
CAAGGACCCGGAGGCTACCCACCGGCCATGCAGCAGCAGCAGCGCATGCA31150.12473616199443313No Hit
CGCAGGCAGTCAATGGCTCCCAGTTCCTGCCGCTGACCGGTCTGCAGGTG30960.12397533147183465No Hit
GGCAGCAACATGCATGGCGACAGCAGTTTCTTGGGTTTTCCGGTTCATGC30740.12309436981408906No Hit
GCTCCTGCGTGCCCCACATGGTGGCCAGTGGGGACTCGGC30620.12261384527350055No Hit
GCCGAGTCCCCACTGGCCACCATGTGGGGCACGCAGGAGC30320.12141253392202929No Hit
GCCAGAGCAGGGTTGAGCTTACCTGGAGAGAGGCTGAGTGCAAATTTGGT30310.12137249021031357No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA30290.12129240278688216No Hit
AGCCAAACTGCCAGGGGTCTGTCCAGGCTCTGGCTGTGAACCCGGAGCAT30260.12117227165173504No Hit
GCTCTGGCTTCTGGGTTTCTGCTAGGTTGTCTGGGGGATCCCAAGGTCCC30080.12045148484085227No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT28700.11492545262408445No Hit
CACCTGCTGCTGCCCAGCTTGCAGATACCTTGTTTAGCAAGGGTCTGGGA27990.1120823490922691No Hit
ACCCCCATCTCTCCTCCTCCGCTTCTCCCTTGGCATCCCACAGCTCTAAA27980.11204230538055342No Hit
CCCATCTGAGCCGCCATCTGGCCCATGGAGCTGCCCTGGAGGGGGAGATG27750.1111213000110921No Hit
GCCACCTCAGGTTGGGTCAGGGATGTGTCCCCCACCAGGGGGCATGAACC27610.11056068804707218No Hit
TACCTGCAGAGCAAGGAATGTGACTTCCAGACCAAATTTGCACTCAGCCT27460.10996003237133654No Hit
CCCGAAAAGCCGTCCAAGGGCTCCGGCTACCGCTGCATTCGCATCAACCA27330.10943946411903233No Hit
CTGGCCTAGGGGAAGACACAGCCCCTCTGGATGGGATTGATGCTCCGGGT26840.1074773222449626No Hit
CCAAAGACTGGCTCGCAGGGCCTCACATGAAAGAGGTCCTACCTGCATCT26580.10643618574035416No Hit
GGAGCCAGGGCTCCCCTGTGGGAAGGGCCTGATGCTGGGGCTGC26510.1061558797583442No Hit
GAACACACTGGCGGTTGTCAATTTCCAGAAAGACTTTAGAGCTGTGGGAT26510.1061558797583442No Hit
GCAGCCCCAGCATCAGGCCCTTCCCACAGGGGAGCCCTGGCTCC26440.10587557377633423No Hit
AGGGGTGGGCACACCGGTCGGCTCTGGTCAGGCCTAGGAGATGCAGGTAG25940.10387338819054881No Hit
GACAAAGACACATCGTTGCTGAAAGAGATTAGGAACAGGTGAGGGTCAGC25730.10303247024451892No Hit
CCAGCCCGAGATGAGGGCTCCCTACGGCTCTGTACTGACTCACTGCCAGA25410.10175107146961623No Hit
CAGGGAGAGGAGGCTACCCGTGAGCTGGAGACTAAGTGGCTGACCCTCAC25090.10046967269471355No Hit
ACTGACCTTGTGGGCCATACTGACCCCCCTGGGGACCATAGCTCCCCATG25010.10014932300098787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)