FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004965415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446695
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN537973.718613805950805No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG84040.5809102817110725No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG59970.41453105181119726No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT50240.3472743045355102No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA43780.30262080120550633No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG42220.29183760225894195No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG33120.22893560840398286No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT31810.2198804862116756No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC30940.2138667791068608No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG30830.2131064253349877No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT29460.20363656472165867No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG28000.19354459647679711No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG27640.19105616595066688No Hit
GTATTTGGAGAAGAATGGAAAGAGAAGACTGATAGAGACTTCGTTAAGGTAAATCATTTTAATTAAAATGTTG27340.18898247384555833No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC27310.18877510463504746No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG27300.18870598156487717No Hit
CAACATTTTAATTAAAATGATTTACCTTAACGAAGTCTCTATCAGTCTTCTCTTTCCATTCTTCTCCAAATAC27150.18766913551232292No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC25180.17405189068877683No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG23370.1615406149879553No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC23350.16140236884761475No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT21990.15200163130445601No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA21270.1470247702521955No Hit
CCTCCTTAGCCCACTGATGAACGATGGCTCCAACTCTGGTAACATTGGAA19750.13651806358631224No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG19750.13651806358631224No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG19570.13527384832324713No Hit
AGAAGGAGGAGCTGCTGTTGGTATAGTGCTGAGGGTTCCAATGTTACCAG19420.13423700227069285No Hit
ACAAAAGCAAGGTGTCACATCCGGAGATAAACATTTATTACAGGGACTGG19410.13416787920052256No Hit
GACTGCTTCCTACATCGTAAGTGCAATTATTGTACGTTTTCATTTTCTAT19400.13409875613035227No Hit
CGAAAACCTCGTGGGAGACCTAGAAGTGGCTCTGACCGAAATTCAGCTAT19320.13354577156899No Hit
CCCCCACTGTGTAAGTGGAATTATTTTTCACTGCAGCATTTCTGAGTCTT19310.1334766484988197No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA19080.13188681788490317No Hit
GAACTTAATTTTACAGTTCAGAGTAAGGAACAAGAAAAAGACTCAGAAAT18780.12981312577979462No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA18490.1278085567448564No Hit
ATATTTTTCTCTTGCAGATTGATTATACCGGAACAGAGCCTTCCAGTCCC18440.12746294139400496No Hit
CAGCTTTCTATCACATAGTGAAGGACGACGCATTGTCGTTATCTGCGGTC18380.12704820297298325No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG18090.125043633938045No Hit
GAGGAGCCACACTTGTCCCCCCAGGCTGAGGGACCACATCTGTCCCCTC17880.12359204946446901No Hit
CCATAACAGCTTTTTTCTCTCCTTGAAGTATTTGTCTGAAAGTGGCCACC17570.12144923428919019No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA17200.11889168069288965No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT17170.1186843114823788No Hit
GAGGGGACAGATGTGGTCCCTCAGCCTGGGGGGACAAGTGTGGCTCCTC17170.1186843114823788No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC16970.11730185007897312No Hit
CCATTTAGTTGACTATCATCTCGAGATTTCACGTAACGACGATGGTTTTGATAGAAATTAGACAGTCCAT16890.11674886551761084No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA16860.11654149630709998No Hit
ATGGACTGTCTAATTTCTATCAAAACCATCGTCGTTACGTGAAATCTCGAGATGATAGTCAACTAAATGG16840.11640325016675941No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC16800.11612675788607828No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC16640.11502078876335371No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT16600.11474429648267256No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA16580.11460605034233201No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC16560.11446780420199143No Hit
CCCCAGGAGGCCAAAGACACAGATCAAGGAGTAGGCAATCGGTACAAATA16530.11426043499148059No Hit
CCTCACACAACACACCAGTGAAACCTTTGGAAAGAATTTTATCAAGGATT16440.11363832735994803No Hit
GCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCG16420.11350008121960745No Hit
CTTTCTAGAATTTTAGTCATACCTGTAGACACCAACTGCCTCCTTAGAAG16350.11301621972841545No Hit
TATTTAGAGGGGAAAACACAGATGGATCTGAGAGGATAGCTGAATTTCGG16330.11287797358807489No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG16330.11287797358807489No Hit
TGCAGGAGGTCAGGCAGTTCTCCAGGCTATTTGTACCGATTGCCTACTCC16320.11280885051790461No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT16190.11191025060569092No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA16140.11156463525483949No Hit
GTTTTCTCTGCAGTCTTTAAACCAGAGGAGTTACGCCAGGCCCTCATGCC16030.11080428148296634No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG16030.11080428148296634No Hit
CAGAAAAAAATGAGACTTGGCCTACCTGCCGGTAGCGTAGCAAATGGCTG16030.11080428148296634No Hit
CTGTAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAG15970.11038954306194465No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT15960.11032041999177436No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA15930.11011305078126349No Hit
TTCTCTGAAGCAAGTTCTGACAGCTTTGTTGAATTCTCGGACTACCAGCC15920.11004392771109323No Hit
GCATTTTAAGACTCTTACCATGTCCTTCATCACACAATCTACATCTTCTG15890.10983655850058235No Hit
GGCATCTAGATCAGTTACTCGGTTGCGAATCAGAATCTAGAAGAGGAGAAAGTACAGAAAAGAGAGTCACCTGG15780.10907620472870923No Hit
ACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGT15780.10907620472870923No Hit
GGCAATAGCTCCACAAGGAGCAATTGGTTTGTCTTCATTTCTTCGATAAG15720.10866146630768751No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG15680.10838497402700638No Hit
GGTGTGGAATATCTGCCTTGGTCTTTGAGAATCCTTGATAAAATTCTTTC15600.10783198946564411No Hit
GAACCCAAATTATACCCCTTGCCAAAAATAAAAATCTTACCCCGCTCATT15580.10769374332530353No Hit
GCCCATGCGCTTCCGGCACTTAAAAAAGACTTCTAAGGAGGCAGTTGGTG15500.10714075876394125No Hit
AGGGCACTCAAATGTCTGCCCTAGCTCCCTGCCCCTCCCCAACACCCAGG15500.10714075876394125No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG15450.10679514341308982No Hit
GGTCAGGTTACGCAGCAAGGCCATGCCAGTTTCTGTCAGCCACACACCTT15390.10638040499206813No Hit
TTTCTACAACCAGCTTGCATTTTTTCTGCCCACAATGAGCGGGGTAAGAT15350.106103912711387No Hit
CCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAA15340.10603478964121671No Hit
CAGAACCAATGGAAGTGGATGAAAAGAAACCTGAAGTGAAAGTAGAAGTT15270.10555092815002472No Hit
GGGAAGCTTTGGTTGGGCAACACATTGTCAAAGTTAAAATCCAATGTATC15160.10479057437815158No Hit
AACAGAAGCTCATGGCCAGCAGGGGGACCAGAAAGCCGATCACCATCTGG15130.10458320516764073No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA15120.10451408209747044No Hit
CTGTAGGAAGAGACACCTCTGTTTCTTGCTGCCCGGGAGGGGAGCTATGA15050.10403022060627845No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC15030.10389197446593788No Hit
GAGAGGCTGTGCCGTTACTGCTACTCTCTTCTTCCTCTTTAACTTCTACT14980.10354635911508644No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA14970.10347723604491615No Hit
ACCATAGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCTGCCAAAGA14940.10326986683440531No Hit
TGTCATCCACGTCCTGGAGCCAGGAGATGAACTGGCGGGCGTTGAAACGG14840.10257863613270246No Hit
TTTAAAAGATGAGAAAGCTCTGAATTCAGAATTTTCCAGTTCTGCAACTT14820.10244038999236191No Hit
CCAGGTGACTCTCTTTTCTGTACTTTCTCCTCTTCTAGATTCTGATTCGCAACCGAGTAACTGATCTAGATGCC14740.10188740543099963No Hit
CTTTTCCTTGTAAAAACCAGAATCCCAGTAAGGAATGTGAACCTTATCGA14670.10140354393980762No Hit
CACAGAGCATGTGGAGGCCTTTATCACCATCCAGGTGGCCCAGATGGTGA14640.10119617472929678No Hit
AGCAGCACCAGCAATCTCAAGACCCACCTGCGACTCCATTCTGGAGAGAA14640.10119617472929678No Hit
CATAAATGAGGATTTTATTGTTTGGATGCGTACTGCAGCATTACCTACTT14630.10112705165912649No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC14540.10050494402759393No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC14510.10029757481708308No Hit
CCAATTTATTTAATTGAAAAGATTGGTTAACATTTTATAGTCAAAGTAAT14490.1001593286767425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)