FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004965416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3186414
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN150500.47231778419251236No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG59980.18823668236456406No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG55230.17332964266413592No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA54370.17063068389732156No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG52480.1646992512586249No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT52310.16416573615355695No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC45870.14395492864392387No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG44960.14109905366973657No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT35210.11050039323201569No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT33660.10563599080345493No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC33520.10519662542281072No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG32630.10240351693157261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)