FastQCFastQC Report
Fri 10 May 2024
EGAF00004965431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257186667
Total Bases38.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG8024910.31202667282903895Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)