FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146636
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN286811.3360905155787939No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG76050.35427524741036676No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG56880.26497272942408495No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG54040.2517427267594506No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT51800.2413077950803024No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA46520.21671117040802448No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT33070.15405499581671042No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG32330.15060774160127754No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA32320.15056115708485276No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG31320.14590270544237588No Hit
AAGGAAATACTACCTTTTCTTAAACGGAAGATGAATGTCAGTGCTACACC30970.144272247367509No Hit
GGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCT30810.1435268951047127No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC30760.14329397252258883No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT28810.13420999181975893No Hit
AGGCAGACATCATGCTAGGTGCTAGGGGACAGTGTTAGACATATTTTAAT28780.13407023827048462No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG27570.1284335117830876No Hit
GCACTTTTAGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTC27490.12806083565168944No Hit
CAGCTTTCTATCACATAGTGAAGGACGACGCATTGTCGTTATCTGCGGTC27100.12624403951112345No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA26830.1249862575676547No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG26580.12382164465703548No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC26540.12363530659133638No Hit
TTTCTACAACCAGCTTGCATTTTTTCTGCCCACAATGAGCGGGGTAAGAT25710.11976879172808058No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT25680.11962903817880628No Hit
GAACCCAAATTATACCCCTTGCCAAAAATAAAAATCTTACCCCGCTCATT25550.11902343946528428No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT25400.11832467171891276No Hit
GCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCG25130.117066889775444No Hit
ACACGGATGGCTTTGTGCCTTTTAGAATTCTGAGCTTGCACCAAGGTTTT25040.11664762912762107No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG24660.11487741750347986No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA24560.11441157233923217No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG24530.11427181878995787No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA24180.11264136071509095No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA24090.11222210006726806No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG24090.11222210006726806No Hit
CCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAA24080.11217551555084326No Hit
ATCGCCTCTATGTGTCTGTGAAGGAGATTGGTGGATTGACTCAGGTGAGT23870.11119724070592313No Hit
CTGTAGGAAGAGACACCTCTGTTTCTTGCTGCCCGGGAGGGGAGCTATGA23800.11087114909094974No Hit
ACGTCTCCTCCAAATGTGTATCTGGAGAGTGATAATAGTATATTAATTTC23710.11045188844312684No Hit
CCATTTCTCCCGGTTCTGGGTTGGAGTCATGGATTTCTTCAGCCCTGCCT23610.10998604327887913No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT23560.1097531206967553No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC23390.10896118391753422No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT23050.10737731035909209No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC23040.10733072584266731No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG23020.10723755680981778No Hit
TGTAAGCCAGCCAGCTCAACAAGGTCAAACTTATGCACCAGCCATTCACC22970.10700463422769393No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC22850.10644562003059672No Hit
CAAATGTTATCTGTGAAAAGGCAGCAACAGCAGCAACATTCACCAGCACC22720.10584002131707472No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC22710.10579343680064994No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC22690.10570026776780041No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC22680.10565368325137564No Hit
GGACACCTATAATACTGAGCTCCTGAAATCAGATTCAGACAATAACAACA22610.10532759163640226No Hit
GGTGGTGGTGGTCTTACCACTCAGGACTTGATGGTCACTGCTTGTCCAGA22590.10523442260355273No Hit
CTTTCAGCCAATAGCAATCCTTGTGAGCATGCAGGAAAATGTGTGAACAC22420.10444248582433165No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG22400.10434931679148211No Hit
CACCCAGCCCAAACTACCAAAAATATACATATGGCCAATCCAGCATGAGC22200.10341762646298674No Hit
ATCCACACACTGCCCATTGTTCACACAAGGGTTGCTCTGACATTCATTTA22180.10332445743013721No Hit
CCAGTCAATTTTGCTGTGAACCTAAAACTGCTCTAAAAAAGTCTTTTTTT22110.10299836581516382No Hit
CTCACAACGAGGTCCTGCATAACCCTTCAGACACTCACAGTGGAAGGCGC22100.10295178129873905No Hit
GGACTTTCAGGTGAATCCCTGACTCCAGAACGCACTCAGCTCCTCTATCT22000.10248593613449136No Hit
ACAAAGTCCATAATGTCTGAAGGCAGGATATTCCCACAGTCATCACTGTT21930.10215984451951798No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC21840.10174058387169506No Hit
GAACCTTCAGGTCTTCCTCTCCATGCACAAATTGAATAATGTTCTTATCC21630.10076230902677492No Hit
ATCCGGAAAAGCTCGAGGACTCTGAAAAAGGGGCCCCGGCAGGATCCCAG21630.10076230902677492No Hit
GGGGCTGCTGAACTTGTACTTGTACCTGCTGTGGTGGGGGTGCTGGTGAA21490.10011012579682817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)