FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146636
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN282151.3143821309248518No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG77030.35884053001999405No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG57900.26972435009941137No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG53710.2502054377174332No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT51620.24046927378465655No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA47330.22048451623843074No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT33710.15703640486789563No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG32650.15209844612687012No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC32010.14911703707568494No Hit
GGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCT31280.14571636737667681No Hit
AAGGAAATACTACCTTTTCTTAAACGGAAGATGAATGTCAGTGCTACACC31130.14501759963030528No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA31070.14473809253175665No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG30200.14068523960280177No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC28580.13313854794198923No Hit
AGGCAGACATCATGCTAGGTGCTAGGGGACAGTGTTAGACATATTTTAAT28290.13178759696567094No Hit
GCACTTTTAGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTC28280.13174101244924616No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT28200.13136833631784803No Hit
GAACCCAAATTATACCCCTTGCCAAAAATAAAAATCTTACCCCGCTCATT27500.1281074201681142No Hit
ACAAAGAACAGGAAGCCACTATGGTAGGAGTGTAACTGAGGACTCACGTG27380.127548405971017No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA26970.12563844079760147No Hit
CAGCTTTCTATCACATAGTGAAGGACGACGCATTGTCGTTATCTGCGGTC26720.12447382788698225No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG26540.12363530659133638No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT26080.12149241883579703No Hit
TTCCAGACCCTCAGAGGCAAAGAAAGCTCATCACAGCTCACGTGAGTCCT25960.12093340463869981No Hit
GTTTAGGTTTCAAAGGTGTGCATTGTGAATTAGAAATAAATGAATGTCAG25870.1205141439908769No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG25450.11855759430103659No Hit
GTGATGTCTCGATTGGCAAAATGGTCTAACAGGATCTTGGCTGCTTCATA25430.11846442526818705No Hit
CTCCTGTGCCGGGAGGTACTCAGAAAACATTTCTCGGCAGACCAGTGAGA25360.11813833365321369No Hit
ACACGGATGGCTTTGTGCCTTTTAGAATTCTGAGCTTGCACCAAGGTTTT25340.11804516462036414No Hit
GCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCG25270.11771907300539075No Hit
TTTCTACAACCAGCTTGCATTTTTTCTGCCCACAATGAGCGGGGTAAGAT25140.11711347429186876No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG24400.11366622007643588No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA24380.11357305104358634No Hit
GGACACCTATAATACTGAGCTCCTGAAATCAGATTCAGACAATAACAACA24340.11338671297788726No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT24000.11180283941944512No Hit
ACGTCTCCTCCAAATGTGTATCTGGAGAGTGATAATAGTATATTAATTTC23890.11129040973877266No Hit
GGTGGTGGTGGTCTTACCACTCAGGACTTGATGGTCACTGCTTGTCCAGA23880.1112438252223479No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC23820.1109643181237993No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC23560.1097531206967553No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG23550.10970653618033054No Hit
CAAATGTTATCTGTGAAAAGGCAGCAACAGCAGCAACATTCACCAGCACC23540.10965995166390577No Hit
CCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAA23410.10905435295038376No Hit
CTGTAGGAAGAGACACCTCTGTTTCTTGCTGCCCGGGAGGGGAGCTATGA23290.10849533875328654No Hit
TTTGAGGTTTCACTGGGCCAGTTTGCCAGATTGATATTGATGACTGTTCC23090.10756364842479116No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC23060.10742389487551686No Hit
CCATTTCTCCCGGTTCTGGGTTGGAGTCATGGATTTCTTCAGCCCTGCCT22780.10611952841562333No Hit
ATCCGGAAAAGCTCGAGGACTCTGAAAAAGGGGCCCCGGCAGGATCCCAG22750.10597977486634903No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC22720.10584002131707472No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC22690.10570026776780041No Hit
ATGTTAGGTTGTCCAGTTAGCTGAACTGTCTGACCAGCTGGAAGAGCTGC22670.10560709873495086No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC22400.10434931679148211No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG22280.10379030259438488No Hit
CTCACAACGAGGTCCTGCATAACCCTTCAGACACTCACAGTGGAAGGCGC22240.10360396452868581No Hit
CACCCAGCCCAAACTACCAAAAATATACATATGGCCAATCCAGCATGAGC22210.10346421097941151No Hit
GTTTTCTCTGCAGTCTTTAAACCAGAGGAGTTACGCCAGGCCCTCATGCC22130.10309153484801335No Hit
TTTGTCCTCTTTGTCAAATGTCACGACTTCGGCCACACAGTTAGGGGCAC22120.10304495033158859No Hit
ATCGCCTCTATGTGTCTGTGAAGGAGATTGGTGGATTGACTCAGGTGAGT21960.10229959806879228No Hit
CTTTCAGCCAATAGCAATCCTTGTGAGCATGCAGGAAAATGTGTGAACAC21890.10197350645381892No Hit
CTTCCAGGGGTTAATTGTGAAATTAATTTTGATGACTGTGCAAGTAACCC21890.10197350645381892No Hit
GACTCATACCTCCATAACTCGACTTATTGTCCTGAAGTGTTTGTATAGGC21840.10174058387169506No Hit
GACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCAT21830.10169399935527029No Hit
ACAAAGTCCATAATGTCTGAAGGCAGGATATTCCCACAGTCATCACTGTT21820.10164741483884553No Hit
TGTAAGCCAGCCAGCTCAACAAGGTCAAACTTATGCACCAGCCATTCACC21760.10136790774029691No Hit
GTCCCCAGATGCCCTGCCTGAATGGAGGGACTTGTGCTGTGGCCAGTAAC21550.10038963289537677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)