FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219547
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN289801.3056718330362007No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG71090.32029058181692033No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG58200.2622156683323219No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG54650.24622141364882114No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG51170.2305425386351359No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT44170.19900457165358518No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC42840.19301235792709054No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA38040.17138632342545573No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT35750.16106890279863414No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT34290.15449098397105357No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT34230.15422065853978312No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC34170.15395033310851267No Hit
GTAGCTGCCCTGGGTGTTATGGCAGAGACCATGGTTCATGCAGGGCTTGG34010.15322946529179152No Hit
TCCTAGGTCAGCGGTGTACCATTGACATTGACGAGTGTATCTCCAAGCCC32410.14602078712457994No Hit
CAAACAGTTGTACAATCTCCTGGGAGGTCCATGACCTCTGCTTGCTCAGT32400.14597573288603485No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA31670.14268677347224457No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA31130.14025384459081064No Hit
CCTCGGTGTCCGAGGACCTGGGGCTGGTGCATTTTCGGTTGTTGCTGATC30710.13836156657191762No Hit
ACAGAGGCCCAGCTGTAGTTCTATTACCAAGTTTTGTACCTTTGTAGCCT30620.13795607842501195No Hit
GTTGTCGTCGTTGCAAATTCTGTCACGTTTGTGGAAGGCAACATCAGGCT30520.13750553603956123No Hit
CGAAGTCCGTGTAGACGGTGTCCTTGAGCAGCGCCCCCGACCCGAAGTCG30460.1372352106082908No Hit
CCTGCTGCCAAGAGGGTCAAGTTGGACAGTGTCAGAGTCCTGAGACAGAT29490.13286494946941876No Hit
CAAGGACGAAAACATCCTTATCGACCTCAATCGCGGCGAGCTCAAGCTCA29290.1319638646985173No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA28490.1283595256149115No Hit
ACAAGTTCAGCAGCCCCAACAAGTACAGATGCAAGTTCAACCTCAACAGT28180.12696284422001428No Hit
CAGACTCGACTCACCAGAGGCAGGCTGACCAACTCCTGCATTCGACTGTT28000.12615186792620295No Hit
CAGAGTGAAACACCCACTTACTTGCAGCACTGCTTCTTTATATTGCGACC27020.12173655254878585No Hit
CCCGAAAAGCCGTCCAAGGGCTCCGGCTACCGCTGCATTCGCATCAACCA26730.12042997963097875No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC26620.11993438300698296No Hit
CATTCCAGCTCCCAAACCCAAAGGTCCTGGAGAACCAGACTCACCAACTC25620.11542895915247571No Hit
ACCCCCATCTCTCCTCCTCCGCTTCTCCCTTGGCATCCCACAGCTCTAAA25580.11524874219829542No Hit
GGCCTTCTTGCTGACAGGTATCATTTCTGTGAGAAGTGTTTCACAGAGAT25520.11497841676702499No Hit
GGCTTACTCAAAATTGGTGGTGTAGGAGGATGCAGAGGAGTTGGTGAGTC25440.1146179828586644No Hit
GACCCCATTGATCGACGGCCATGGACACAGCAGCACCTTGAAGCTGCAGA25440.1146179828586644No Hit
CCAAAGGGAAGGATGGGCTACATCACCCCCTTGCTTCTGCAGTCCAAACA25430.11457292862011932No Hit
CGCCTTGTCTCCTACAGGGAAACCAATGAAAAGCGTGCTGGTGGTGGCTC25350.11421249471175875No Hit
GGCGCAATCATGACACAGTGAGAAATCATGAGACCACTGTGCATCCCACC25100.11308613874813193No Hit
CCACCTCCTGCTCTGCCTGAGGAGGGGTGAGAGCCAGCGATGGTGTCCTA25030.11277075907831643No Hit
CCATTAGCTCTGTTGTATCCCGGTGAGGGCAGCCTCTGGCTTGGGCAGGG24880.11209494550014035No Hit
GAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGGTAA24840.11191472854596006No Hit
GGGGCTGTGAAGGGTCGTCACCCAGGGTCACATTCTCGCCCTGGATCTCT24810.11177956583032483No Hit
GCCAATGCCCAGGACAACATGGGCCGCTGTCCACTCCATGCTGCAGTGGC24450.11015761324270222No Hit
CTTCTCATGTTCTTTACCTGGAAGACACCTTGGGCATCAGCTGCCACTGC24210.1090763115176205No Hit
CTGTCCCTACACAGCAAGTTCATTCGTGTGCAGAATGAAGGAACTGGAAA24110.10862576913216976No Hit
ACTAATTGCTTAGATAAGCCCAAGTTTGGTGGTCGCAATATAAAGAAGCA24060.1084004979394444No Hit
GTTCGCTGTAAGAGCTGTGGATCCACAACTCCAGGCAAAGGGTGGGATGC24010.10817522674671903No Hit
GAACACACTGGCGGTTGTCAATTTCCAGAAAGACTTTAGAGCTGTGGGAT23830.10736425045290772No Hit
AAAGGCCTCCACATGCTCTGTGATGAGAGAGCATCGCATCGCTTGCTCAC23790.10718403349872743No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG23740.10695876230600208No Hit
TATCCAAGGACACATGGATGCAGACCACATCTCTAGCCCTCCTTGTGGTTCAGTAGAGCAAGGTCATGGCAACA23580.10623789448928093No Hit
CCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGG23570.10619284025073586No Hit
TGTTGCCATGACCTTGCTCTACTGAACCACAAGGAGGGCTAGAGATGTGGTCTGCATCCATGTGTCCTTGGATA23440.1056071351496499No Hit
TGCCACCCTCTGGATTGAGCATCCACCAAGAACTTTTTCCAGTTCCTTCA23360.10524670124128932No Hit
TGCCCTCCTCACTCACCTGATTCTGGTGGTGGAGGCTGCTTTTTCTTGGG23300.1049763758100189No Hit
CCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAA23120.10416539951620758No Hit
CTCCAGGCAGTGGCAGTGCTGGAAGCTTGAGTAGGCTCCATCCAGTCCCA23070.10394012832348222No Hit
CACCTGCTGCTGCCCAGCTTGCAGATACCTTGTTTAGCAAGGGTCTGGGA22940.10335442322239628No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG22880.10308409779112583No Hit
CAGCCCTGCCAGAATGGAGGCACCTGTATTGACCTTGTGAACCATTTCAA22780.10263355540567512No Hit
ACCCCGAGTGCCTGGTGGGCAAGAGCACTTGAAATGGTTCACAAGGTCAA22770.10258850116713004No Hit
GCCTGTTCTCTGCTTCCCCTTACCTAGGTATGACAAATACTGTGCAGACC22680.1021830130202244No Hit
CAAATGGTGGGAACAGACGAGATGCCTCCGGTGGTGGAGACATGGGTGAC22640.10200279606604411No Hit
ACCAAACATCTCATTGTACTGGGTCTCATTCACCTCCATGCGGTTCATCC22430.10105665705659758No Hit
ACAGCATTTGCCATCCACATACGCAAGAGCCGACTTAAGTGGGCAGTCAG22360.10074127738678208No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG22320.1005610604326018No Hit
ACAGGACCGCCAAGAAAAGAAGTTCCCAAAACCACTCCTAGTGAGCCCAA22270.10033578923987643No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG22250.1002456807627863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)