FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219547
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN282521.2728723473753878No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG70520.31772249021985116No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG57380.2585212207716259No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG54140.24392364748302242No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG50640.22815466399224707No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT45060.20301439888409661No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC42090.1896332900362101No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA37720.1699445877920134No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT35350.15926673325683124No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT35290.15899640782556082No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT34180.15399538734705776No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC33990.1531393568147014No Hit
GTAGCTGCCCTGGGTGTTATGGCAGAGACCATGGTTCATGCAGGGCTTGG33220.14967018044673078No Hit
TCCTAGGTCAGCGGTGTACCATTGACATTGACGAGTGTATCTCCAAGCCC32320.14561529897767428No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA31870.14358785824314602No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA31560.14219117684824875No Hit
CAAACAGTTGTACAATCTCCTGGGAGGTCCATGACCTCTGCTTGCTCAGT30960.1394879225355444No Hit
CGAAGTCCGTGTAGACGGTGTCCTTGAGCAGCGCCCCCGACCCGAAGTCG30750.1385417835260979No Hit
GTTGTCGTCGTTGCAAATTCTGTCACGTTTGTGGAAGGCAACATCAGGCT30530.1375505902781063No Hit
ACAGAGGCCCAGCTGTAGTTCTATTACCAAGTTTTGTACCTTTGTAGCCT30150.13583852921339354No Hit
CCTGCTGCCAAGAGGGTCAAGTTGGACAGTGTCAGAGTCCTGAGACAGAT30070.13547809530503296No Hit
CCTCGGTGTCCGAGGACCTGGGGCTGGTGCATTTTCGGTTGTTGCTGATC29620.1334506545705047No Hit
CAAGGACGAAAACATCCTTATCGACCTCAATCGCGGCGAGCTCAAGCTCA28670.1291705019087228No Hit
CAGACTCGACTCACCAGAGGCAGGCTGACCAACTCCTGCATTCGACTGTT28600.1288551222389073No Hit
CTGGCTGGCCACCCTGCTGATGATGGGGTCCATCTTGTGGTTGATGCGAA28400.12795403746800585No Hit
ACAAGTTCAGCAGCCCCAACAAGTACAGATGCAAGTTCAACCTCAACAGT27890.12565627130220716No Hit
GGCTTACTCAAAATTGGTGGTGTAGGAGGATGCAGAGGAGTTGGTGAGTC27200.12254752884259716No Hit
ACTAATTGCTTAGATAAGCCCAAGTTTGGTGGTCGCAATATAAAGAAGCA26190.11799705074954485No Hit
CCCGAAAAGCCGTCCAAGGGCTCCGGCTACCGCTGCATTCGCATCAACCA25840.11642015240046731No Hit
GACCCCATTGATCGACGGCCATGGACACAGCAGCACCTTGAAGCTGCAGA25600.11533885067538556No Hit
GGCCTTCTTGCTGACAGGTATCATTTCTGTGAGAAGTGTTTCACAGAGAT25400.11443776590448411No Hit
CCCACAAGGCGTCCTCTTCATCCTTCAGCGTCTCCATGTAGTCATCCATC25260.1138070065648531No Hit
GAACACACTGGCGGTTGTCAATTTCCAGAAAGACTTTAGAGCTGTGGGAT25210.11358173537212775No Hit
CCACCTCCTGCTCTGCCTGAGGAGGGGTGAGAGCCAGCGATGGTGTCCTA25120.11317624722522207No Hit
CAGAGTGAAACACCCACTTACTTGCAGCACTGCTTCTTTATATTGCGACC24980.11254548788559107No Hit
CCAAAGGGAAGGATGGGCTACATCACCCCCTTGCTTCTGCAGTCCAAACA24940.11236527093141079No Hit
CCATTAGCTCTGTTGTATCCCGGTGAGGGCAGCCTCTGGCTTGGGCAGGG24930.11232021669286571No Hit
GGGGTTTATGTCTAAAAGGAGGAGGGCCAGAGATGGATGACTACATGGAG24850.11195978278450514No Hit
GTTCGCTGTAAGAGCTGTGGATCCACAACTCCAGGCAAAGGGTGGGATGC24720.11137407768341918No Hit
GGGGCTGTGAAGGGTCGTCACCCAGGGTCACATTCTCGCCCTGGATCTCT24680.1111938607292389No Hit
ACCCCCATCTCTCCTCCTCCGCTTCTCCCTTGGCATCCCACAGCTCTAAA24580.11074331834378817No Hit
GAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGGTAA24550.11060815562815296No Hit
TGCCACCCTCTGGATTGAGCATCCACCAAGAACTTTTTCCAGTTCCTTCA24510.11042793867397267No Hit
CTCCAGGCAGTGGCAGTGCTGGAAGCTTGAGTAGGCTCCATCCAGTCCCA24510.11042793867397267No Hit
GCCAATGCCCAGGACAACATGGGCCGCTGTCCACTCCATGCTGCAGTGGC24240.10921147423325571No Hit
CTTCTCATGTTCTTTACCTGGAAGACACCTTGGGCATCAGCTGCCACTGC24180.10894114880198526No Hit
CCATCCCAGAGCTCCTGTACAGTGACCTCCAGAGGAGCAGCAGTGAGCAA23950.10790490131544861No Hit
ACAGGACCGCCAAGAAAAGAAGTTCCCAAAACCACTCCTAGTGAGCCCAA23930.10781479283835846No Hit
GGCGCAATCATGACACAGTGAGAAATCATGAGACCACTGTGCATCCCACC23810.10727414197581758No Hit
CATTCCAGCTCCCAAACCCAAAGGTCCTGGAGAACCAGACTCACCAACTC23510.10592251481946541No Hit
GGCAGAGAACCTCACCTGGAAAATGACAAGGAGAGCCACCACCAGCACGC23410.10547197243401468No Hit
CCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGG22990.10357969441512165No Hit
CGCCTTGTCTCCTACAGGGAAACCAATGAAAAGCGTGCTGGTGGTGGCTC22990.10357969441512165No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG22980.10353464017657657No Hit
CAGCCCTGCCAGAATGGAGGCACCTGTATTGACCTTGTGAACCATTTCAA22850.10294893507549062No Hit
ACAGCATTTGCCATCCACATACGCAAGAGCCGACTTAAGTGGGCAGTCAG22810.10276871812131033No Hit
ACCCCGAGTGCCTGGTGGGCAAGAGCACTTGAAATGGTTCACAAGGTCAA22800.10272366388276526No Hit
AAAGGCCTCCACATGCTCTGTGATGAGAGAGCATCGCATCGCTTGCTCAC22750.10249839269003991No Hit
GCTCTGGCTTCTGGGTTTCTGCTAGGTTGTCTGGGGGATCCCAAGGTCCC22660.10209290454313426No Hit
GCCTGTTCTCTGCTTCCCCTTACCTAGGTATGACAAATACTGTGCAGACC22480.10128192824932294No Hit
ACCACCTCATGTTCCTCCTAAGAGAAGTGGCCTTACCTTGTCTTTCACAT22470.10123687401077787No Hit
CTGTCCCTACACAGCAAGTTCATTCGTGTGCAGAATGAAGGAACTGGAAA22450.10114676553368773No Hit
TATCCAAGGACACATGGATGCAGACCACATCTCTAGCCCTCCTTGTGGTTCAGTAGAGCAAGGTCATGGCAACA22430.10105665705659758No Hit
CGCCTGTCTCCACCACCTGAGGAGTCACCCATGTCCCCTCCACCTGAAGA22410.10096654857950745No Hit
TGTTGCCATGACCTTGCTCTACTGAACCACAAGGAGGGCTAGAGATGTGGTCTGCATCCATGTGTCCTTGGATA22370.10078633162532716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)