FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908564
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2177327.485893382438895No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG92300.31733872797710483No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG92120.31671986588570855No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC87690.3014889821918995No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG67960.2336548207294046No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA65840.22636600054184813No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT59450.20439639629727932No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG51800.17809475741293643No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT50960.17520673431975367No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG50600.17396901013696106No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG49460.17004955022478446No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA40260.13841882110897336No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT33130.11390500604421977No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA32690.11239223204302878No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG32440.11153270136053392No Hit
CCTCTTTTAAATCATTCTTCTTCTCTAATTTTGAGGGCCGGTCTTTTGATTTCTTCTTTCTCTCCTCTTTGTAC32440.11153270136053392No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG31790.10929792158604727No Hit
GGTTCCGTGTGCTTTAACAGTGCGCCTTCTTTGCCTCCTAGTTTCGTTGA30680.10548160535577007No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT29620.10183719526199182No Hit
GTACAAAGAGGAGAGAAAGAAGAAATCAAAAGACCGGCCCTCAAAATTAGAGAAGAAGAATGATTTAAAAGAGG29510.10145900176169408No Hit
GCATCTTCCGGCCACACTCCTTGCAATCAACGAAACTAGGAGGCAAAGAA29460.10128709562519513No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG29190.10035880248810067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)