FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908564
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2178797.4909474228519635No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG94090.3234929676637681No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC92400.3176825402501028No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG87630.3012826948281007No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG66860.2298728857264272No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA65040.22361550235786457No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT60290.2072844193904621No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG52100.17912619423193027No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG49520.17025583758858323No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG49410.16987764408828548No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT48330.16616447153990765No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA40940.14075674456535942No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG33790.11617416704600621No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG33070.113698718680421No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT32300.1110513641783368No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA31180.10720066672075979No Hit
GTACAAAGAGGAGAGAAAGAAGAAATCAAAAGACCGGCCCTCAAAATTAGAGAAGAAGAATGATTTAAAAGAGG31130.10702876058426083No Hit
GCATCTTCCGGCCACACTCCTTGCAATCAACGAAACTAGGAGGCAAAGAA30850.10606608621986657No Hit
CCTCTTTTAAATCATTCTTCTTCTCTAATTTTGAGGGCCGGTCTTTTGATTTCTTCTTTCTCTCCTCTTTGTAC29980.10307491944478445No Hit
GGTTCCGTGTGCTTTAACAGTGCGCCTTCTTTGCCTCCTAGTTTCGTTGA29520.10149338298899388No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT29340.10087452089759757No Hit
ACCCACACGTTTTTCCAAGCAAATATCCAACATCTGAGAAAAGTCTCGTT29100.1000493714424025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)