FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004965444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219264
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN151596068.3091331180067No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG128600.579471392317453No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC124780.5622584784865613No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACACTAAGATATCTCGTATGC67140.302532731572269No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCTGTTG37680.16978601914869074No Hit
GCACAACCACGCGGAGACGAGAACGCCTGACACGCACGGCACGG37150.16739784000461413No Hit
CAACAGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC36960.16654170031145463No Hit
CCGTGCCGTGCGTGTCAGGCGTTCTCGTCTCCGCGTGGTTGTGC33990.15315888510785558No Hit
GCTGTTGTTGTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG29180.1314850328757642No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACTAAGATATCTCGTAT29090.13107949302110972No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAACAACAACAGC29050.13089925308570768No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCG28770.12963757353789362No Hit
CGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC28440.1281505940708271No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG27500.12391495558887991No Hit
GAGGGGACAGATGTGGTCCCTCAGCCTGGGGGGACAAGTGTGGCTCCTC25220.11364127927096551No Hit
CCGGCATTTCTTGGCGGTCCTGTCTGTCTCTTAACACATCTCCGAGCCCA24610.1108926202560849No Hit
ACAGGACCGCCAAGAAATGCCGGCTGTCTCTTAACACATCTCCGAGCCCA23780.10715264159649326No Hit
AGGAGGGAGCAAAGGAGTCGAGGTATATCTCCCGGTT22270.10034858403506748No Hit
GAGGAGCCACACTTGTCCCCCCAGGCTGAGGGACCACATCTGTCCCCTC22240.10021340408351596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)