FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004965445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219264
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN148715667.01122534317683No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC124210.5596900594070827No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG115780.5217044930211097No Hit
TCTCTTATACACATCTGACGCTGCCGACGATCGATATCGTGTAGATCTCG38620.1740216576306379No Hit
CAACAGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC37220.16771325989156766No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCTGTTG34500.15545694428423115No Hit
CCGTGCCGTGCGTGTCAGGCGTTCTCGTCTCCGCGTGGTTGTGC34470.15532176433267966No Hit
GCACAACCACGCGGAGACGAGAACGCCTGACACGCACGGCACGG31990.1441468883377552No Hit
CCGGCATTTCTTGGCGGTCCTGTCTGTCTCTTAACACATCTGACGCTGCC29950.13495465163225287No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAACAACAACAGC28800.12977275348944514No Hit
CGCAGCAACAGCTTCAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC28410.12801541411927558No Hit
GCTGTTGTTGTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG27110.12215761621871034No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG26840.1209409966547468No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTGAAGCTGTTGCTGCG26620.11994967701003575No Hit
ACAGGACCGCCAAGAAATGCCGGCTGTCTCTTAACACATCTGACGCTGCC25470.11476777886722807No Hit
GAGGAGCCACACTTGTCCCCCCAGGCTGAGGGACCACATCTGTCCCCTC22230.10016834409966548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)