FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450954
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN145110.5920551752501271No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG68860.2809518252892547No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG59250.24174260308435No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG49560.20220697736473225No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA41110.16773060612316673No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG41060.16752660392647106No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT37770.15410325938389705No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT35150.14341354427704478No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT35060.1430463403229926No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC30440.12419653734831416No Hit
CACCGTGAAGCCCCGTTTCCTGGCTATGAAACGTGGGCTCTGCCAGATCC26740.10910037479283576No Hit
GCCAAGCGGAATGCTGAGCCTGGCCTTGGCACCCACAGGTAGTGCTTGTT26690.10889637259614011No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA25730.10497953041958355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)