FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450954
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN144340.588913541421014No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG66710.27217973083134156No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG55090.2247696203192716No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG50400.20563421426921924No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA40250.1642217683400015No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG39180.15985612133071447No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT37370.1524712418103318No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT36400.14851359919443613No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT34020.138803094631723No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC31340.12786857688883593No Hit
CACCGTGAAGCCCCGTTTCCTGGCTATGAAACGTGGGCTCTGCCAGATCC26870.10963078050424448No Hit
GCCAAGCGGAATGCTGAGCCTGGCCTTGGCACCCACAGGTAGTGCTTGTT26550.10832516644539228No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA25960.10591794052438357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)