FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004965450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882062
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN139201.5781203588863368No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG26400.29929868875430526No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG18990.21529098861531276No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG18080.2049742535105242No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC15530.1760647210740288No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG15210.17243685818003723No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT14030.15905911375844328No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA11460.12992283989107342No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT11310.12822227915951487No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG10200.11563812974598159No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG9660.10951611111237078No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA9610.10894925753518461No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA9570.10849577467343566No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG9510.10781555038081223No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG9010.1021470146089504No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG8960.1015801610317642No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG8910.10101330745457802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)