FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004965451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882062
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN137571.5596409322700673No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG24220.27458387278898766No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG19900.22560772372010132No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG17350.19669819128360588No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG15470.17538449678140539No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC14980.16982933172498077No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT14070.15951259662019224No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT11790.13366407350050225No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA10830.12278048481852749No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA9970.11303060329092512No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG9750.11053644755130593No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG9410.10668184322643988No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG9410.10668184322643988No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA9200.10430105820225788No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG9140.10362083390963447No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT9130.10350746319419722No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG9050.10260049747069934No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG8830.10010634173108013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)