FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004965454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886488
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN291343.2864517060580627No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG36500.41173710191226504No Hit
GCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCG31740.35804207163548746No Hit
ATAATAATTCTTACCATTGACTTTCACAGTAATTCGCTTGTAGTCGGCAC26970.30423423667325444No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG26170.29520986183682124No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT20960.23643862071455No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG18500.20868866809251788No Hit
CAACATTTTAATTAAAATGATTTACCTTAACGAAGTCTCTATCAGTCTTCTCTTTCCATTCTTCTCCAAATAC17940.20237160570701465No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG17600.19853624640153053No Hit
GTATTTGGAGAAGAATGGAAAGAGAAGACTGATAGAGACTTCGTTAAGGTAAATCATTTTAATTAAAATGTTG17200.19402405898331393No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG17140.19334723087058145No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG17090.19278320744330438No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC16880.19041430904874065No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT16530.18646614505780112No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC16520.18635334037234572No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG16400.18499968414688073No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA16250.1833076138650495No Hit
CCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAA15300.17259116874678507No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG15260.1721399500049634No Hit
GGTGGTGGTGGTCTTACCACTCAGGACTTGATGGTCACTGCTTGTCCAGA14750.16638691104673722No Hit
CAGGCCTCTCATCTATAATAATTCTTACCATTGACTTTCACAGTAATTCG14060.1586033877503136No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC13920.15702412215393777No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC13720.15476802844482948No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG13500.15228632536481035No Hit
GGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCT13120.1479997473175046No Hit
AAGGAAATACTACCTTTTCTTAAACGGAAGATGAATGTCAGTGCTACACC12820.14461560675384214No Hit
AGAAGAAAGAATAGAATGTTCTCTCTATAAGAAAATTGCATGCATTTTAA12720.14348755989928796No Hit
CCATTTAGTTGACTATCATCTCGAGATTTCACGTAACGACGATGGTTTTGATAGAAATTAGACAGTCCAT12280.13852415373924973No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT11580.13062782575737067No Hit
GCTGAACGGTAAGACACCAAATCCTTCCATTAGGTTCTATATTTTAAATA11550.13028941170100442No Hit
GTATGTTTAATTATTAAGTGAAGCAGTCTTCTTTTCGTGTTTTCCATAAT11420.12882295079008402No Hit
ATGGACTGTCTAATTTCTATCAAAACCATCGTCGTTACGTGAAATCTCGAGATGATAGTCAACTAAATGG11410.12871014610462858No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA11300.12746929456461903No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA11270.12713088050825277No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT11250.12690527113734196No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT11250.12690527113734196No Hit
CAAACTGATCCTTTGAAATTGTCCTTGTTTTAAAATAAGAAAACATGTTTTATTAACTAAAATTTATCTGTTG10870.12261869309003617No Hit
GAAGTGGACTTTGCTATTAACGTATGCACTCTCCTATCAAATGAAAGCAA10770.12149064623548204No Hit
CTTACCGTTCAGCAAATGCCAGTAGGTCATGAATATAAAGACAGCAAATA10510.11855772441364124No Hit
CAACAGATAAATTTTAGTTAATAAAACATGTTTTCTTATTTTAAAACAAGGACAATTTCAAAGGATCAGTTTG10510.11855772441364124No Hit
AGCACATTACTTGGAAACACAGACCTCAAATGACTTTTCCAGTGTGAAGT10310.11630163070453295No Hit
AGGCAGACATCATGCTAGGTGCTAGGGGACAGTGTTAGACATATTTTAAT10310.11630163070453295No Hit
CTTTCTAGAATTTTAGTCATACCTGTAGACACCAACTGCCTCCTTAGAAG10240.11551199790634503No Hit
CCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGG10230.11539919322088962No Hit
TAGGATCTTTTTCAAGTTGCATGACGTGCTTGCTTTCATTTGATAGGAGA10130.11427114636633547No Hit
GCACTTTTAGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTC10030.11314309951178131No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC9990.11269188076995966No Hit
CGAAAACCTCGTGGGAGACCTAGAAGTGGCTCTGACCGAAATTCAGCTAT9970.11246627139904883No Hit
CTCCGGATGTGACACCTTGCTTTTGTACCATTAACTTCACACTGGAAAAG9960.11235346671359342No Hit
GACTGCTTCCTACATCGTAAGTGCAATTATTGTACGTTTTCATTTTCTAT9950.112240662028138No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT9900.11167663860086094No Hit
ACAGCATTTGCCATCCACATACGCAAGAGCCGACTTAAGTGGGCAGTCAG9710.10953334957720805No Hit
GAGGGGACAGATGTGGTCCCTCAGCCTGGGGGGACAAGTGTGGCTCCTC9590.10817969335174306No Hit
GCCCATGCGCTTCCGGCACTTAAAAAAGACTTCTAAGGAGGCAGTTGGTG9530.10750286523901056No Hit
CTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGGGTAAGTTCTTGCTTTGTTCAA9520.10739006055355514No Hit
GTCTCCTCCAGGGGGATTTCTGAATTTCACATTTTTATCTGTCCACCAAG9460.10671323244082266No Hit
GAACTTAATTTTACAGTTCAGAGTAAGGAACAAGAAAAAGACTCAGAAAT9310.10502116215899145No Hit
GGGAAGCTTTGGTTGGGCAACACATTGTCAAAGTTAAAATCCAATGTATC9270.10456994341716977No Hit
CCCTGCATCCATGGAAACTGTACTGGAGGTCTCAGTGGGTGAGTAGCTGC9250.10434433404625894No Hit
CAAACAGTTGTACAATCTCCTGGGAGGTCCATGACCTCTGCTTGCTCAGT9250.10434433404625894No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA9180.10355470124807103No Hit
GAACCTTCAGGTCTTCCTCTCCATGCACAAATTGAATAATGTTCTTATCC9180.10355470124807103No Hit
CCCCCACTGTGTAAGTGGAATTATTTTTCACTGCAGCATTTCTGAGTCTT9170.10344189656261564No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA8980.10129860753896275No Hit
AGAAGGAGGAGCTGCTGTTGGTATAGTGCTGAGGGTTCCAATGTTACCAG8960.10107299816805192No Hit
GAAAAAGAGGGACCAATAACTAAACACATCCGACTAACAGCTGCCTTAAT8950.10096019348259648No Hit
GACAATTGAATGCAAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCAC8900.10039617005531942No Hit
ACCTATCGCTTTGAAAAAGAAAGGTATTGCTTGGTGGACAGATAAAAATG8880.10017056068440859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)