FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004965455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886488
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN284383.207939644981094No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG36590.41275234408136374No Hit
GCAGGGGTGTACCGCTGCATGATCAGCTATGGTGGTGCCGACTACAAGCG35600.4015846802212777No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG26190.2954354712077321No Hit
ATAATAATTCTTACCATTGACTTTCACAGTAATTCGCTTGTAGTCGGCAC24410.2753562371966682No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT19960.2251581521690085No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG17660.19921307451426304No Hit
GTATTTGGAGAAGAATGGAAAGAGAAGACTGATAGAGACTTCGTTAAGGTAAATCATTTTAATTAAAATGTTG17310.1952649105233235No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT17020.19199357464511646No Hit
CAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCCTCTTC16940.19109113716147313No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA16850.1900758949923744No Hit
CCAGCCAGTCACCCAGAAGATGCCGCAGGTGTTGGGG16820.18973748093600817No Hit
CAACATTTTAATTAAAATGATTTACCTTAACGAAGTCTCTATCAGTCTTCTCTTTCCATTCTTCTCCAAATAC16760.18906065282327567No Hit
CAACAGCAGCAACAGCAGCTACAACAGCAACAGCAAC16680.18815821533963237No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG16600.18725577785598904No Hit
GTTGCTGTTGCTGTTGTAGCTGCTGTTGCTGCTGTTG16550.18669175442871194No Hit
CCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGG15940.17981066861593162No Hit
GGTGGTGGTGGTCTTACCACTCAGGACTTGATGGTCACTGCTTGTCCAGA15200.17146312189223092No Hit
CCAAGCTGCTTTAAAACATAATTCCACAACAAAGATAGAAAATGAAAACG15110.17044787972313216No Hit
CCAGTCACCTCTGAACATGAACTGACATGTCAGGCTGAGGGCTACCCCAA14800.16695093447401432No Hit
CCAAGGTCTTTAGAAAAACTTGAAATTATTCCTGCAAGCCAATTTTGTCC14060.1586033877503136No Hit
CTTCTTACCTTGCACTCATAGAGAACACATACTCCAGAAGAGGAATAGAC13800.1556704659284728No Hit
CAGATGGGATTTCACTCACATGATCTCAACACGTGGACAAAATTGGCTTG13490.15217352067935494No Hit
GGCACATCCTCCAAATGAAAGGACTCACTTGGTAATTCTGGGAGCCATCT13120.1479997473175046No Hit
AAGGAAATACTACCTTTTCTTAAACGGAAGATGAATGTCAGTGCTACACC12900.14551804423748546No Hit
GCTGAACGGTAAGACACCAAATCCTTCCATTAGGTTCTATATTTTAAATA12100.13649366940105223No Hit
AGAAGAAAGAATAGAATGTTCTCTCTATAAGAAAATTGCATGCATTTTAA12090.13638086471559682No Hit
CAGGCCTCTCATCTATAATAATTCTTACCATTGACTTTCACAGTAATTCG11980.13514001317558727No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT11870.13389916163557772No Hit
ATGGACTGTCTAATTTCTATCAAAACCATCGTCGTTACGTGAAATCTCGAGATGATAGTCAACTAAATGG11800.1331095288373898No Hit
GCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGAT11790.1329967241519344No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA11760.1326583100955681No Hit
CCATTTAGTTGACTATCATCTCGAGATTTCACGTAACGACGATGGTTTTGATAGAAATTAGACAGTCCAT11130.12555161491187697No Hit
ACAAGCAGCACATCAGGTAAAGTGAGATGACAAATGATCTCTCTGTAGGA11020.12431076337186742No Hit
GTATGTTTAATTATTAAGTGAAGCAGTCTTCTTTTCGTGTTTTCCATAAT10930.12329552120276868No Hit
GAAGTGGACTTTGCTATTAACGTATGCACTCTCCTATCAAATGAAAGCAA10790.12171625560639288No Hit
CTTACCGTTCAGCAAATGCCAGTAGGTCATGAATATAAAGACAGCAAATA10710.12081381812274955No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT10660.12024979469547248No Hit
CAACAGATAAATTTTAGTTAATAAAACATGTTTTCTTATTTTAAAACAAGGACAATTTCAAAGGATCAGTTTG10560.11912174784091832No Hit
AGCACATTACTTGGAAACACAGACCTCAAATGACTTTTCCAGTGTGAAGT10530.11878333378455208No Hit
AGGCAGACATCATGCTAGGTGCTAGGGGACAGTGTTAGACATATTTTAAT10290.11607602133362212No Hit
GACTGCTTCCTACATCGTAAGTGCAATTATTGTACGTTTTCATTTTCTAT10280.11596321664816671No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT10280.11596321664816671No Hit
CAAACTGATCCTTTGAAATTGTCCTTGTTTTAAAATAAGAAAACATGTTTTATTAACTAAAATTTATCTGTTG10210.11517358384997879No Hit
GCACTTTTAGGAGATTAGATCCTGAGGAAAACCATACAGCTGAATTGGTC10170.11472236510815714No Hit
CTTTCTAGAATTTTAGTCATACCTGTAGACACCAACTGCCTCCTTAGAAG10130.11427114636633547No Hit
ACCTATCGCTTTGAAAAAGAAAGGTATTGCTTGGTGGACAGATAAAAATG10080.1137071229390584No Hit
CTCCGGATGTGACACCTTGCTTTTGTACCATTAACTTCACACTGGAAAAG9980.11257907608450425No Hit
CCAGTGTGAAACTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGG9970.11246627139904883No Hit
ACAGCATTTGCCATCCACATACGCAAGAGCCGACTTAAGTGGGCAGTCAG9800.11054859174630678No Hit
TAGGATCTTTTTCAAGTTGCATGACGTGCTTGCTTTCATTTGATAGGAGA9770.11021017768994054No Hit
CGAAAACCTCGTGGGAGACCTAGAAGTGGCTCTGACCGAAATTCAGCTAT9750.1099845683190297No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC9740.10987176363357429No Hit
CGATACACTTTGCCCCATTCAGACACGGAGTACTGGAACAGTCATCAATA9610.10840530272265389No Hit
GCCCATGCGCTTCCGGCACTTAAAAAAGACTTCTAAGGAGGCAGTTGGTG9480.10693884181173349No Hit
GACAATTGAATGCAAATTCCCAGTAGAAAAACAATTAGACCTGGCTGCAC9450.10660042775536725No Hit
TTGAACAAAGCAAGAACTTACCCCAAACAATTAGTGGAATTGGCAAAAGAAGAAGACAAAGCCACCCCAAGTTAG9350.1054723809008131No Hit
GAACTTAATTTTACAGTTCAGAGTAAGGAACAAGAAAAAGACTCAGAAAT9350.1054723809008131No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC9210.10389311530443729No Hit
GAGGAGCCACACTTGTCCCCCCAGGCTGAGGGACCACATCTGTCCCCTC9190.10366750593352644No Hit
TGGTGGATACAGATGCGAGGTGAGGACACTGAGCAAGCAGAGGTCATGGA9140.10310348250624937No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG9140.10310348250624937No Hit
GAACCTTCAGGTCTTCCTCTCCATGCACAAATTGAATAATGTTCTTATCC9030.1018626309662398No Hit
CCTCCTTAGCCCACTGATGAACGATGGCTCCAACTCTGGTAACATTGGAA8970.10118580285350733No Hit
CCCCCACTGTGTAAGTGGAATTATTTTTCACTGCAGCATTTCTGAGTCTT8940.10084738879714107No Hit
GTTTCGAGGAATTTTCCCTTTTCTGAAGATTGTCCTTCTTTCTTTTTAGC8890.100283365369864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)