FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435874
Sequences flagged as poor quality0
Sequence length35-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2082518.549333832538137No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG131690.5406273066669294No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG85560.35124969518127785No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG83240.34172539302114974No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG75370.30941666112450805No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT73060.2999334119909322No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG69130.28379957255588756No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA54770.22484742642681846No Hit
CACACCCCAATGGCGGGATAGCAGCCCCTGTGTGTGAGCACCCCTCCATG50330.2066198826376077No Hit
AGAGAGGTATAGAGGGGATAACAGATGCCGAGTACCTGGCATGGAGGGGT49290.20235036787617094No Hit
GAGCTGTGCGTAGCGCATGGCTAAGTTGTAGTTGTCGTCCTTCACCTTGA48430.19881980759267515No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC47620.19549451244194074No Hit
GAAGGAAGAGCGGGACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACG46060.18909024029978563No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT44390.18223438486555543No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA43550.1787859306351642No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT41670.17106796164333624No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG40230.1651563258198084No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT39290.16129734132389442No Hit
GACCCAGTCCCGGGTGCTCAGTTCCCCCCAGCTGGCACAGCAGGGTCAGG38930.15981943236801247No Hit
GAGAGGCACGTACCATCCAGATCTAAGGCGTCAAACCCGCCGCTGTCATT38370.15752046288108498No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG37200.15271725877446862No Hit
GACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCAT37200.15271725877446862No Hit
CTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTA37140.15247094061515495No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC37110.15234778153549813No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC36850.15128040284513894No Hit
TCCAGTGTGATGATGGTGAGGATGGGCCTCCGGTTCATGCCGCCCATGCA36770.15095197863272072No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC36470.14972038783615244No Hit
GCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAATT35440.14549192610126796No Hit
CTCTGAAATCACCTGTTAACGATGTTATTGAGCCGGCTGGCTTGTGGGAT35120.1441782292515951No Hit
CACCCACAGGCGGATGTCCGCGTCGCCCTCTGTGCTTTGCTGGTGGATCC35000.1436855929329678No Hit
CCCGCAGCAGGGCGTGGAGGGGGCTGGGGAGGAAATCCTTGGGAGCCCCG34020.1396623963308447No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG33950.13937502514497876No Hit
GAACTTAGGCCTCCCTGACCTCGTGGCAAAGTACAACACCAATAACGGGG33850.138964494879456No Hit
GGAACACCTCACCCCCTTCCCATGTGGCATCTTTCACAAGGTCCAGGTGA33760.1385950176404855No Hit
CAGGGGACAGATGCGATTCCTCAGGCCGGGGGGACAGGCATGGCTCCTCA33740.13851291158738097No Hit
CCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAA33210.1363371011801103No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG33130.1360086769676921No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA32670.13412023774628737No Hit
CCCCACCAGGCTGGCTTCCTGTCAGGCGAGCTGGCCCTCAACAGCCCCGG31970.131246525887628No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT31840.13071283654244842No Hit
CGCACCCCCTCCCTCCCTGGCGGAGCCCCTGAAGGAGCTGTTCAGGCAGC31830.13067178351589614No Hit
CATGCGGTGCTTGTACATTTCACAGTCCTTTCCCAGGGTCGAGCACTTGA31640.1298917760114029No Hit
GGGACTGCTGAGACTGGGGCTGAGGATACGCAGCCTGCTGGGAGAGCGTC31570.12960440482553695No Hit
GAGGAGCCATGCTTGTCCCCCCAACCTGAGGAATCACACCTGTCCCCCCA31450.12911176850690964No Hit
CAGCCTCCTCCTGCTGCTGCTTCAAGATATACTTTTGGCAATCCTCCTCT31210.128126495869655No Hit
GAACTTGTATTTCTTCCAGTTGCAGGCTTTGGGGTCAGTGGGGCTCTTGG31140.12783912468378905No Hit
GACAGGCGTGATGCCTCAGGTGGTGGGGACGTGGGTGATTCCTCAGGTGG31090.12763385955102768No Hit
ATGTCGAAAAGTGTTTCTGTCATCCAAATACTCCACACGCAAATTTCCTT31060.12751070047137086No Hit
GCTGCCCCCACCTGAGGAGTCACCCCTGTCCCCACCACCTGAGGAATCAC30680.12595068546238433No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA30560.12545804914375702No Hit
GTGGCCCACGCCCTGCATGCCAAAGGGCTGGCTGTTGTAGCTGAGGTCCA30500.12521173098444335No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA30480.1251296249313388No Hit
GGAACAGACAAACTATCGACTGAAGTTGTGTGTGTCTGACCGCGATGTCC30450.12500646585168199No Hit
GTGGGCAGCCTCAACATAGGAGATGTCCAGCTGGAGAAGCCGTGACACCC30420.12488330677202515No Hit
AGGACATCTCTGATTGGAGGGGGGACTCTTGCTCATAGGAGATGATGGTC30290.12434961742684555No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG30090.12352855689580003No Hit
CCCTGACTCTGTCCCACTGCCCAGGTACATTAACCATTCCTGTGCCCCTA30050.12336434478959091No Hit
AGGAGGGGTAGTACTGGGAGGTCCCCTTCATGCCCGAAGGGGACAGGGGC29870.12262539031164994No Hit
TTTGTCCTCTTTGTCAAATGTCACGACTTCGGCCACACAGTTAGGGGCAC29870.12262539031164994No Hit
ACCCGCTCGATGCTGTGCTGTAGACGCAGCTTTCCCCGGACGGCCTCCTG29840.12250223123199311No Hit
CAGGTGGAGGGGACATGGGTGACTCCTCAGGTGGTGGAGACAGGCGAGAT29710.12196854188681351No Hit
GAGACACTGGAGCTGGGTGCACAGCAGGCGGGGGCAGCCTGGCAGGAGTA29630.1216401176743953No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC29600.12151695859473849No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA29570.12139379951508165No Hit
CCCCGCGCGGTGAAGGGCGTCAGGTGCAGCTGGCTGGACATCTCGGCGAA29210.11991589055919968No Hit
CTGACGATGGAGTCGTTTCCCGGGGGCTCGGCGGTGATTGACATGATGCT29000.11905377700160188No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA28920.11872535278918367No Hit
CCACGCTGCCTTCCTGGAGGAAGCTCAATGGACCCAACCAGCTGATGTCG28740.11798639831124269No Hit
CCACCTGACCCTCCGCCAGGCCGGCGACGACTTCTCCCGCCGCTACCGCC28680.11774008015192905No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA28410.11663164843501758No Hit
TCCCAGCCCACGGAACCAACTGGCATTCAGCCAGGAGAGAGAACTGTCCT28300.11618006514294253No Hit
GCCACATGGAGGGGAGTATTGCCGTGCCTGTCTTGATAATCGACGAAACA28270.1160569060632857No Hit
GTCCCCGCCACCTGAGGAATTGCCCACTTCCCCGCCACCTGAAGCATCTC28190.11572848185086748No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA28040.11511268645258335No Hit
ACACGGATGGCTTTGTGCCTTTTAGAATTCTGAGCTTGCACCAAGGTTTT27990.11490742131982197No Hit
CCGAAGCTCCGCTGATGCGGTTGTCCGACAGGTCCACGTAGCGCAGGCCA27960.11478426224016514No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC27910.11457899710740375No Hit
GCGTCTGCAGATGAACTTCATCAACCAGGCCCAGCTCGGCATCTTCAGGG27830.11425057289498554No Hit
AAGAATGCCTGCATCCTCCAGGCTTCTGGCTCCCCTCCAGCCAAGAGCCC27820.11420951986843325No Hit
TCAGCCACCTGAGCCGTCTTGAAGGCCTGGTGTTGAAGGACAGTTCTCTC27790.11408636078877642No Hit
GCACCCGTAACGTGTGTGTGTCTGTGTCTGAAGCAGCCCCCCCGGAGCCG27440.11264950485944676No Hit
CCCTCAGCAAAAGGCCAAAGATAACCGGGCAGCTCACCGCATCAACAAGG27440.11264950485944676No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC27360.11232108064702855No Hit
TCACAGTACCTGGAGGAGAAGGAGGACCTGGAGCTCAAGTGCTCGACCCT27230.11178739130184895No Hit
TCGATGAGCTCACTAGCAATAGACCAGACACAGGTGCCAGGCAGGACATC26920.11051474747872839No Hit
TCGATTTTTATTTTTATTTTTAAAGAGGGAGACGATGGACTGAGCTGATC26820.1101042172132056No Hit
GAACCAATCAAGCATTGCCCAAGATCGAGGTGAGAGCCTGGGTGTTGGGG26770.10989895208044421No Hit
GGTCAGGTTACGCAGCAAGGCCATGCCAGTTTCTGTCAGCCACACACCTT26580.10911894457595098No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG26410.10842104312456227No Hit
GACTCCTCCCCAGCCAGCAGCTCCCACTCAGCCATCAACTCCTGTGTCGT26410.10842104312456227No Hit
CGCTGGCCAGGGGGTTCAGCTGGTCCTGCACGTCGTACACAAACTGCTGC26300.10796945983248722No Hit
CGCACAGCAGCCTCTCTTCATCCACATTCCTGGGACCGGGACTCGGAGGT26050.10694313416868033No Hit
CCGGCTGGATGCCGCCAGGAGGGCCTTCGGCTGGGGCGGCGGCACGGGAG26010.10677892206247121No Hit
GCCCTCGAGGCCACGGGATTCTTCATCTGCAATATCTGGACCATCATCTC25980.10665576298281437No Hit
AGGGGTGCTCTGGGTTTGGGTAGCACTGGGCACTGAGCCAGGAGTCGGGG25840.10608102061108252No Hit
GTGGTCGTGGTCTTCATAGTCTTCCAGCTGCCCTACAATGGGGTGGTCCT25750.10571154337211201No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG25720.10558838429245518No Hit
ATGGAGGTCCTTATGACAGAGTGAGGTAAGCATGACCCCAGCTCCTGTCC25670.10538311915969381No Hit
CACATGTGCCCGGCGGGGTTGTGGGCTTCGCTCAGGCCTGGGTGGGGAGC25620.10517785402693243No Hit
CGGTGCGCAGCCGCCGGTACTGAGATGACTTGGAGTGCACAAACTGCTTG25340.10402836928346869No Hit
AACACCCAGGGCAGCTACATGTGTGAATGTCCACCAGGCTTCAGTGGTAT25180.10337152085863226No Hit
AACAGAAGCTCATGGCCAGCAGGGGGACCAGAAAGCCGATCACCATCTGG24930.10234519519482534No Hit
CAAATGTTATCTGTGAAAAGGCAGCAACAGCAGCAACATTCACCAGCACC24920.10230414216827308No Hit
GGCACAAGCTCAGGCCGCAGACGGTGCCGGCCCCGAGGACGACACTGAGG24900.10222203611516853No Hit
GAGCCCACTATAGCTTCAGGGTGCTGGGGGAGTGGACAGGAGCTGGGGTC24840.10197571795585486No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT24760.10164729374343665No Hit
GTCCCCAGATGCCCTGCCTGAATGGAGGGACTTGTGCTGTGGCCAGTAAC24720.10148308163722754No Hit
CACCCAGCCCAAACTACCAAAAATATACATATGGCCAATCCAGCATGAGC24680.10131886953101843No Hit
AAAGAGGTAGGCACTCCCAGGGAGGCTGCTTTACTCTGTCTCTTCCCCAC24670.10127781650446616No Hit
CGTTCAGGTGGCCCACCTCCGTGCCCATGTGCTTATCCACACTGGTGAGA24650.10119571045136161No Hit
AGGCGATGTTGAGTTGCTTACTGAGCTCACAGGTGCTACTGGTGATGTTG24550.10078518018583885No Hit
CAGTAAGACCCGAGACTCCATGGTGCGGATCAGCTCAGTCCATCGTCTCC24520.10066202110618201No Hit
GACAGAGAGAGTAGTTCTCAAACCACTCCGAGTTGGGATTCAGTTCTGCT24510.10062096807962972No Hit
GAACAGCACCAGCATGTCTAAGGCATATCAGAGCACCTTCACAGGCGAGA24480.10049780899997292No Hit
GGACAGCAACAAGGGTCAAGGTAATCACAATCACCATCTACCTGCTGTGT24410.10021043781410696No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT24370.10004622570789787No Hit
GACTCATACCTCCATAACTCGACTTATTGTCCTGAAGTGTTTGTATAGGC24360.10000517268134558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)