FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435874
Sequences flagged as poor quality0
Sequence length35-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2055328.437710653342496No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG126690.5201007933907912No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG88160.36192348208486974No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG82710.339549582613879No Hit
GTGGCTGCGAGAGTGGCTTCAAGGACAAGTCCGACGGCGACTTTGGGAAG72270.2966902228933024No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT71980.29549968512328634No Hit
CTCGTGCTGGGTGGTGCCGTTCCCACGGCTCCAGGCCCTTCCCAAAGTCG70760.2904912158839086No Hit
GAGACAGGCAGGCATCGGTCCATTGGCACTCCTTACCTAAAGGAAGGATA55390.22739271407305964No Hit
AGAGAGGTATAGAGGGGATAACAGATGCCGAGTACCTGGCATGGAGGGGT49550.20341774656653014No Hit
CACACCCCAATGGCGGGATAGCAGCCCCTGTGTGTGAGCACCCCTCCATG49490.20317142840721647No Hit
GAAGGAAGAGCGGGACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACG48500.1991071787785411No Hit
CCTTCTCACAACAGCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCC47730.19594609573401578No Hit
GAGCTGTGCGTAGCGCATGGCTAAGTTGTAGTTGTCGTCCTTCACCTTGA45490.18675021778630585No Hit
ACAACAACCTCACTGTGGTGCCCCGCAACCTGCCTTCCAGCCTGGAGTAT43220.1774311807589391No Hit
ACCACCACACTCACCTTTCCACGGTCATCTTGATGGTAGCTGGGACATAA42910.17615853693581854No Hit
CAGGCGCCAGTTTGACGATGCGGTTGTAGGACAACAGCAGATACTCCAGG41500.17037006019194753No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT41150.16893320426261788No Hit
GCCACAGGACCTGCAGCCTGCCCACTCTCCCCTAGGTGCCGCCGGATGGT39050.1603120686866398No Hit
TCCAGTGTGATGATGGTGAGGATGGGCCTCCGGTTCATGCCGCCCATGCA37690.15472885707553016No Hit
CAAGAGGCACACGCTGCAGACCCACAGTGACAAACCCTACAAGTGTGACC37320.15320989509309593No Hit
GACCCAGTCCCGGGTGCTCAGTTCCCCCCAGCTGGCACAGCAGGGTCAGG37240.15288147088067774No Hit
GACCTCCAGTCCCCAACCAAGCTGAGTGCCAGCGGGGAGGACTCCACCAT37190.15267620574791635No Hit
GAGAGGCACGTACCATCCAGATCTAAGGCGTCAAACCCGCCGCTGTCATT37170.1525940996948118No Hit
CAACAGTGTCCTTCATTCCCCCACAGAACTGCCCAAGATGGAATCCAAGG37120.1523888345620504No Hit
CTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTA36780.15099303165927302No Hit
GTGGCTGGCGAGGTTGCCCTTGTAGCGGAAGGAGGCCTGGCAGCGGTCAC36770.15095197863272072No Hit
CAGAGCCCAGAACATCCTTGAAGAGCTGCTGCAGGTCCTTGGATTCCATC36530.1499667059954661No Hit
ATGTGCCCTGTGCACCCTCACCTTGTGTCAATGGAGGCACCTGTCGGCAG35610.14618982755265666No Hit
CTCTGAAATCACCTGTTAACGATGTTATTGAGCCGGCTGGCTTGTGGGAT35290.14487613070298383No Hit
GCTCTTAGGTCTGGCCCCTCCTCAGCATCTTATCCGAGTGGAAGGAAATT35100.14409612319849055No Hit
CACCCACAGGCGGATGTCCGCGTCGCCCTCTGTGCTTTGCTGGTGGATCC34280.14072977502120387No Hit
GAACTTAGGCCTCCCTGACCTCGTGGCAAAGTACAACACCAATAACGGGG34220.14048345686189023No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG33980.1394981842246356No Hit
GGAACACCTCACCCCCTTCCCATGTGGCATCTTTCACAAGGTCCAGGTGA33830.13888238882635143No Hit
GAGGAGCCATGCTTGTCCCCCCAACCTGAGGAATCACACCTGTCCCCCCA33570.13781501013599226No Hit
CCCGCAGCAGGGCGTGGAGGGGGCTGGGGAGGAAATCCTTGGGAGCCCCG33220.13637815420666258No Hit
GACAGGCGTGATGCCTCAGGTGGTGGGGACGTGGGTGATTCCTCAGGTGG33190.13625499512700576No Hit
TGCTCTGCAGGTAATCATCAGAGACAACCACCACCATCCGGCGGCACCTA32980.13539288156940796No Hit
TTTGTCCTCTTTGTCAAATGTCACGACTTCGGCCACACAGTTAGGGGCAC32410.1330528590559282No Hit
CCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAA32180.13210863944522583No Hit
CGCACCCCCTCCCTCCCTGGCGGAGCCCCTGAAGGAGCTGTTCAGGCAGC32140.1319444273390167No Hit
GGGACTGCTGAGACTGGGGCTGAGGATACGCAGCCTGCTGGGAGAGCGTC31400.12890650337414825No Hit
ATGTCGAAAAGTGTTTCTGTCATCCAAATACTCCACACGCAAATTTCCTT31280.12841386705552094No Hit
CCCCACCAGGCTGGCTTCCTGTCAGGCGAGCTGGCCCTCAACAGCCCCGG30880.12677174599342989No Hit
GTGGCCCACGCCCTGCATGCCAAAGGGCTGGCTGTTGTAGCTGAGGTCCA30580.12554015519686157No Hit
CAAAGCGCCGTAGCTCCCGCTGCCCGTGTAGACTGGGCTCCCCGCAGTTA30500.12521173098444335No Hit
ATTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCT30410.12484225374547288No Hit
AGGAGGGGTAGTACTGGGAGGTCCCCTTCATGCCCGAAGGGGACAGGGGC30410.12484225374547288No Hit
CACAGTAGGTGATCCTTTACTCTCCTCTGGACTTCGAAGCATTGGCTCCA30320.12447277650650239No Hit
CATGCGGTGCTTGTACATTTCACAGTCCTTTCCCAGGGTCGAGCACTTGA30290.12434961742684555No Hit
GGAACAGACAAACTATCGACTGAAGTTGTGTGTGTCTGACCGCGATGTCC30280.1243085644002933No Hit
CAGGGGACAGATGCGATTCCTCAGGCCGGGGGGACAGGCATGGCTCCTCA30200.12398014018787508No Hit
GCTGCCCCCACCTGAGGAGTCACCCCTGTCCCCACCACCTGAGGAATCAC30200.12398014018787508No Hit
GTCCACAGTGGGGGTCCGGTGCGGCGTTGTGCTCTCTGTAACTGCGGGGA30000.12315907965682954No Hit
GAGACACTGGAGCTGGGTGCACAGCAGGCGGGGGCAGCCTGGCAGGAGTA29990.12311802663027727No Hit
GTGGGCAGCCTCAACATAGGAGATGTCCAGCTGGAGAAGCCGTGACACCC29840.12250223123199311No Hit
CAGCCTCCTCCTGCTGCTGCTTCAAGATATACTTTTGGCAATCCTCCTCT29760.12217380701957489No Hit
AAGAATGCCTGCATCCTCCAGGCTTCTGGCTCCCCTCCAGCCAAGAGCCC29710.12196854188681351No Hit
TCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTTTTA29700.12192748886026125No Hit
TCCCAGCCCACGGAACCAACTGGCATTCAGCCAGGAGAGAGAACTGTCCT29470.12098326924955888No Hit
AGGACATCTCTGATTGGAGGGGGGACTCTTGCTCATAGGAGATGATGGTC29150.11966957239988604No Hit
GAACTTGTATTTCTTCCAGTTGCAGGCTTTGGGGTCAGTGGGGCTCTTGG29110.11950536029367692No Hit
ACCCGCTCGATGCTGTGCTGTAGACGCAGCTTTCCCCGGACGGCCTCCTG29100.11946430726712466No Hit
GTCCCCGCCACCTGAGGAATTGCCCACTTCCCCGCCACCTGAAGCATCTC28960.11888956489539279No Hit
CAACAAGAAGGCGGCCTCTGGGGAAGCCAAGCCTAAGGCTAAAAAGGCAG28760.11806850436434727No Hit
GGACCGCTGGGGTGATAAGGAAGAGGTACTGTGACGCCTGGAGCCAATGC28700.1178221862050336No Hit
CCACCTGACCCTCCGCCAGGCCGGCGACGACTTCTCCCGCCGCTACCGCC28550.11720639080674945No Hit
GCCAGGATTCTCAAAGTAACAACTATCAGAATCTTCCAGTACAGGACAGA28380.11650848935536075No Hit
CAGGTGGAGGGGACATGGGTGACTCCTCAGGTGGTGGAGACAGGCGAGAT28320.11626217119604708No Hit
GCACCCGTAACGTGTGTGTGTCTGTGTCTGAAGCAGCCCCCCCGGAGCCG28310.11622111816949482No Hit
CCCCGCGCGGTGAAGGGCGTCAGGTGCAGCTGGCTGGACATCTCGGCGAA28230.1158926939570766No Hit
CCCTGACTCTGTCCCACTGCCCAGGTACATTAACCATTCCTGTGCCCCTA28060.1151947925056879No Hit
GGTCAGGTTACGCAGCAAGGCCATGCCAGTTTCTGTCAGCCACACACCTT28020.11503058039947879No Hit
TCGATTTTTATTTTTATTTTTAAAGAGGGAGACGATGGACTGAGCTGATC28010.11498952737292652No Hit
TCACAGTACCTGGAGGAGAAGGAGGACCTGGAGCTCAAGTGCTCGACCCT27930.1146611031605083No Hit
GCGTCTGCAGATGAACTTCATCAACCAGGCCCAGCTCGGCATCTTCAGGG27880.11445583802774692No Hit
TTGTAAGTTCGAAATGGGCACAGTTTGTACTCCCCCTGAGGGTGGCATTG27880.11445583802774692No Hit
CCACGCTGCCTTCCTGGAGGAAGCTCAATGGACCCAACCAGCTGATGTCG27720.11379898960291049No Hit
AAAAGAGCCATCCTCCTGAGGTTTGGGGCCATCTGGAAGCATTAGGTTTC27660.11355267144359682No Hit
GCCACATGGAGGGGAGTATTGCCGTGCCTGTCTTGATAATCGACGAAACA27590.1132653002577309No Hit
AGAAAGGGGTTTGTGGAAACCACAGGGGCAGTCAAGTGTCAGGCGCCCAC27580.11322424723117862No Hit
GCCCTCGAGGCCACGGGATTCTTCATCTGCAATATCTGGACCATCATCTC27570.11318319420462634No Hit
CCGAAGCTCCGCTGATGCGGTTGTCCGACAGGTCCACGTAGCGCAGGCCA27250.1118694973549535No Hit
CGCTGGCCAGGGGGTTCAGCTGGTCCTGCACGTCGTACACAAACTGCTGC27100.11125370195666935No Hit
ACACGGATGGCTTTGTGCCTTTTAGAATTCTGAGCTTGCACCAAGGTTTT27100.11125370195666935No Hit
ATGGAGGTCCTTATGACAGAGTGAGGTAAGCATGACCCCAGCTCCTGTCC27050.11104843682390797No Hit
CCCTCAGCAAAAGGCCAAAGATAACCGGGCAGCTCACCGCATCAACAAGG27050.11104843682390797No Hit
CCTCTTATGCAGAGCCCCAGTACTTTCGCTTCGAAGGTGTGTGGCTGACA27030.11096633077080341No Hit
CTGACGATGGAGTCGTTTCCCGGGGGCTCGGCGGTGATTGACATGATGCT26790.10998105813354879No Hit
TCGATGAGCTCACTAGCAATAGACCAGACACAGGTGCCAGGCAGGACATC26660.10944736878836919No Hit
TCAGCCACCTGAGCCGTCTTGAAGGCCTGGTGTTGAAGGACAGTTCTCTC26290.10792840680593496No Hit
GACTCCTCCCCAGCCAGCAGCTCCCACTCAGCCATCAACTCCTGTGTCGT25990.10669681600936666No Hit
AAAGAGGTAGGCACTCCCAGGGAGGCTGCTTTACTCTGTCTCTTCCCCAC25760.1057525963986643No Hit
GTGGTCGTGGTCTTCATAGTCTTCCAGCTGCCCTACAATGGGGTGGTCCT25700.10550627823935063No Hit
CACCCAGCCCAAACTACCAAAAATATACATATGGCCAATCCAGCATGAGC25560.10493153586761876No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG25560.10493153586761876No Hit
AGGGGTGCTCTGGGTTTGGGTAGCACTGGGCACTGAGCCAGGAGTCGGGG25550.1048904828410665No Hit
GAAACGGCGAGAAGAGGAAAAGGTGTCAGGTGTGGTTGGAGACCCCCAGA25390.10423363441623007No Hit
CCGGCTGGATGCCGCCAGGAGGGCCTTCGGCTGGGGCGGCGGCACGGGAG25300.10386415717725957No Hit
GAACAGCACCAGCATGTCTAAGGCATATCAGAGCACCTTCACAGGCGAGA25140.10320730875242315No Hit
CAAATGTTATCTGTGAAAAGGCAGCAACAGCAGCAACATTCACCAGCACC25020.10271467243379584No Hit
GACAGAGAGAGTAGTTCTCAAACCACTCCGAGTTGGGATTCAGTTCTGCT24820.10189361190275031No Hit
GAACCAATCAAGCATTGCCCAAGATCGAGGTGAGAGCCTGGGTGTTGGGG24820.10189361190275031No Hit
CGTTCAGGTGGCCCACCTCCGTGCCCATGTGCTTATCCACACTGGTGAGA24800.10181150584964574No Hit
CACATGTGCCCGGCGGGGTTGTGGGCTTCGCTCAGGCCTGGGTGGGGAGC24720.10148308163722754No Hit
CTGGGTCCTGTCGATACAGTGCTTCTAGGGTTGGCATGAGGGCCTGGCGT24680.10131886953101843No Hit
GGCACAAGCTCAGGCCGCAGACGGTGCCGGCCCCGAGGACGACACTGAGG24640.10115465742480932No Hit
CACAGAGCATGTGGAGGCCTTTATCACCATCCAGGTGGCCCAGATGGTGA24620.10107255137170477No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT24570.1008672862389434No Hit
GTCCCCAGATGCCCTGCCTGAATGGAGGGACTTGTGCTGTGGCCAGTAAC24540.10074412715928656No Hit
CGGTGCGCAGCCGCCGGTACTGAGATGACTTGGAGTGCACAAACTGCTTG24530.1007030741327343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)