FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004965468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004965468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2539792
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82280.3239635371715479No Hit
GCCAGAATGGTGGGACTTGTGTGGCCCAGGCCATGCTGGGGAAAGCCACG42140.16591909888683798No Hit
ACCTGGAAGGCAGTTGCACTCAAAAGTGAAGTCACCAGTCTGCCGACAGG39060.1537921215595608No Hit
CCCCCAAGAAGCCTTCTGGACATCTGTATGGAAAAGAGAAGAGTCCATGA33290.13107372572242137No Hit
CGAGACACAAAGCATGGATGAGATGTCGAGTACTGGCAGTCCTCTCCTGT33240.13087685920736816No Hit
CACACTCATTGACATCAGTCTCACACTTCTGCCCTGTGAAGCCTGTGAGG32850.1293413003899532No Hit
GGACCGATGCCTGCCTGTCTCATCCCTGTGCAAATGGAAGTACCTGTACC32200.12678203569426158No Hit
AATTTTTTGGACTTACAAGAAGTCAGGTGTTTTCATGGACTCTTCTCTTT31990.12595519633103813No Hit
CGCAAAAAGAAAATATATGAAGAGAAAAACTTACTTGCAGTATCCTGTGC31780.12512835696781469No Hit
ATGTGCCCTGTGCACCCTCGCCTTGTGTCAATGGAGGCACCTGTCGGCAG31580.12434089090760188No Hit
GTCATGATTGCGCCAAGCTCTTTGCTAAAGGTACCCAAAAAAGCCAGTTT31170.12272658548416564No Hit
AGCACATTACTTGGAAACACAGACCTCAAATGACTTTTCCAGTGTGAAGT30300.11930110812223993No Hit
CCAGAACACCAAGTACAACCTCCGAAAGCTGGCAAAACTGGCTTTTTTGG29450.1159543773663355No Hit
CTCCGGATGTGACACCTTGCTTTTGTACCATTAACTTCACACTGGAAAAG28930.11390696560978222No Hit
AGCATTGATATTCTCTGATTCTAACCTTTTACCCACCCACATACCTGCAC27930.1099696353087182No Hit
GGGGCCATCTCCTATTTCTGTGGCCAGTACTGAGTTTTGTTATCCTTCCT27190.10705601088593082No Hit
CAGGTGACCCAAAATCCAGCAAATGAACAAGAAAGTGCAGGTATGTGGGT26430.10406363985712215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)